Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis
Abstract
:1. Introduction
2. Results
2.1. P. koraiensis Somatic Embryo Development
2.2. Proliferation Differences between Embryogenic Cell Lines of P. koraiensis
2.3. Differences in the Number of ESEs between Different Cell Lines of P. koraiensis
2.4. Differences in Soluble Protein, Soluble Sugar, and Starch Contents between Different Cell Lines of P. koraiensis
2.5. Differences in Endogenous Hormone Contents between Different Cell Lines of P. koraiensis
2.6. Differences in Endogenous Polyamine Contents between Different Cell Lines of P. koraiensis
2.7. Indicator Verification
3. Discussion
4. Materials and Methods
4.1. Embryogenic Tissue Induction
4.2. Maturation of Somatic Embryos
4.3. Screening of Morphological and Physiological Markers of High-Yield Cell Lines of Somatic Embryos
4.3.1. Microscopic Observation
4.3.2. Fresh Weight and Proliferation Efficiency
4.3.3. Physiological Index Determination
4.3.4. Morphophysiological Marker Verification
4.3.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cell line | Cotyledonary SEs | PEMIII | ESE | Pro | Sta | ABA |
---|---|---|---|---|---|---|
SE·g −1 FW | Number | Number | ng·g −1 FW | ng·g −1 FW | ng·g −1 FW | |
2-2 | 2.86 ± 1.90 | 2.00 ± 0.67 | 2.60 ± 0.43 | 0.78 ± 0.08 | 1.59 ± 0.08 | 409.84 ± 1.52 |
3-5 | 5.71 ± 2.33 | 1.80 ± 0.33 | 2.80 ± 0.33 | 1.08 ± 0.03 | 1.32 ± 0.01 | 413.28 ± 3.51 |
m5-4 | 0 ± 0 | 0.50 ± 0.31 | 1.00 ± 0.21 | 0.60 ± 0.01 | 2.18 ± 0.00 | 293.36 ± 2.82 |
m13-4 | 25.71 ± 7.62 | 1.70 ± 0.65 | 0.90 ± 0.48 | 0.91 ± 0.05 | 1.59 ± 0.02 | 438.78 ± 5.11 |
m28-11 | 0 ± 0 | 0.30 ± 0.21 | 0.10 ± 0.10 | 0.35 ± 0.02 | 4.03 ± 0.01 | 276.72 ± 3.78 |
z3 | 0 ± 0 | 2.60 ± 0.37 | 0.10 ± 0.10 | 0.30 ± 0.00 | 4.43 ± 0.02 | 441.68 ± 1.18 |
z4 | 0 ± 0 | 2.40 ± 0.48 | 0.20 ± 0.13 | 0.48 ± 0.03 | 3.03 ± 0.03 | 353.77 ± 1.96 |
z20 | 0 ± 0 | 1.90 ± 0.43 | 0.80 ± 0.29 | 0.55 ± 0.00 | 2.48 ± 0.03 | 512.76 ± 0.72 |
z21 | 157.14 ± 19.05 | 3.80 ± 0.65 | 4.40 ± 0.62 | 0.61 ± 0.02 | 1.23 ± 0.01 | 264.78 ± 3.38 |
Cell line | IAA | Put | Spm | Spd | Put:Spd | Put:Spm |
ng·g −1 FW | ng·g −1 FW | ng·g −1 FW | ng·g −1 FW | |||
2-2 | 56.32 ± 0.54 | 232.01 ± 1.68 | 1761.09 ± 8.33 | 23.31 ± 0.16 | 9.96 ± 0.12 | 0.132 ± 0.001 |
3-5 | 69.43 ± 0.19 | 221.41 ± 1.03 | 1153.37 ± 24.49 | 17.25 ± 0.19 | 12.85 ± 0.19 | 0.193 ± 0.004 |
m5-4 | 59.71 ± 0.19 | 193.57 ± 0.90 | 1385.82 ± 8.49 | 16.27 ± 0.11 | 11.90 ± 0.05 | 0.140 ± 0.001 |
m13-4 | 37.83 ± 0.39 | 228.61 ± 2.17 | 2376.09 ± 9.52 | 25.52 ± 0.15 | 8.96 ± 0.05 | 0.096 ± 0.001 |
m28-11 | 67.09 ± 0.1 | 192.70 ± 2.41 | 1201.47 ± 5.95 | 22.40 ± 0.24 | 8.62 ± 0.19 | 0.160 ± 0.002 |
z3 | 59.83 ± 0.42 | 135.12 ± 2.03 | 1484.19 ± 15.58 | 13.20 ± 0.10 | 10.24 ± 0.15 | 0.091 ± 0.002 |
z4 | 53.79 ± 0.38 | 230.11 ± 0.63 | 1491.48 ± 16.14 | 14.00 ± 0.18 | 16.46 ± 0.17 | 0.154 ± 0.002 |
z20 | 68.91 ± 0.45 | 251.78 ± 2.28 | 1462.33 ± 11.07 | 14.02 ± 0.08 | 17.96 ± 0.22 | 0.172 ± 0.002 |
z21 | 44.71 ± 0.26 | 201.16 ± 1.29 | 2315.61 ± 12.07 | 21.42 ± 0.06 | 9.39 ± 0.04 | 0.087 ± 0.001 |
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Peng, C.; Gao, F.; Wang, H.; Tretyakova, I.N.; Nosov, A.M.; Shen, H.; Yang, L. Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis. Plants 2022, 11, 1867. https://doi.org/10.3390/plants11141867
Peng C, Gao F, Wang H, Tretyakova IN, Nosov AM, Shen H, Yang L. Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis. Plants. 2022; 11(14):1867. https://doi.org/10.3390/plants11141867
Chicago/Turabian StylePeng, Chunxue, Fang Gao, Hao Wang, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, and Ling Yang. 2022. "Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis" Plants 11, no. 14: 1867. https://doi.org/10.3390/plants11141867