Co-Transcriptomic Analysis of the Maize–Western Corn Rootworm Interaction
Abstract
:1. Introduction
2. Results
2.1. The Maize Transcriptomic Response Varies from Tissues and Time Points
2.2. Differentially Expressed Genes Vary within Tissues and Have Minimal Overlap
2.3. Genes Were Co-Expressed in Tissues and/or Time Points
2.3.1. Roots: M1, M3, M8
2.3.2. Leaves: M4, M5, M6
2.3.3. Time Points: M2, M7, M9, M10
2.4. Modulations in Maize Hormone Pathways during WCR Infestation
2.4.1. Ethylene (ET)
2.4.2. Jasmonic Acid (JA)
2.4.3. Salicylic Acid (SA)
2.5. Maize DIMBOA Pathway Was Turned Off after Extended WCR Feeding
2.6. WCR Transcriptome Is Remodeled between 1 and 5 dpi
2.6.1. 0 h vs. 1 dpi
2.6.2. 0 h vs. 5 dpi
3. Discussion
4. Materials and Methods
4.1. Insect Growth
4.2. Plant Growth and WCR Infestations
4.3. RNA Extraction and RNA-Seq Libraries Construction and Sequencing
4.4. Analysis of RNA-Seq Libraries
4.5. Functional Annotation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pingault, L.; Basu, S.; Vellichirammal, N.N.; Williams, W.P.; Sarath, G.; Louis, J. Co-Transcriptomic Analysis of the Maize–Western Corn Rootworm Interaction. Plants 2022, 11, 2335. https://doi.org/10.3390/plants11182335
Pingault L, Basu S, Vellichirammal NN, Williams WP, Sarath G, Louis J. Co-Transcriptomic Analysis of the Maize–Western Corn Rootworm Interaction. Plants. 2022; 11(18):2335. https://doi.org/10.3390/plants11182335
Chicago/Turabian StylePingault, Lise, Saumik Basu, Neetha N. Vellichirammal, William Paul Williams, Gautam Sarath, and Joe Louis. 2022. "Co-Transcriptomic Analysis of the Maize–Western Corn Rootworm Interaction" Plants 11, no. 18: 2335. https://doi.org/10.3390/plants11182335