Physiological Responses of Cigar Tobacco Crop to Nitrogen Deficiency and Genome-Wide Characterization of the NtNPF Family Genes
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification and Characterization of the NPF Genes in N. tabacum
2.2. Phylogenetic Analysis of the NPFs in N. tabacum
2.3. Gene Structure and Conserved Motif Analysis of the NPFs in N. tabacum
2.4. Chromosomal Location and Syntenic Analysis of the NPFs in N. tabacum
2.5. Analysis of Cis-Elements in NPF Promoters in N. tabacum
2.6. Transcriptional Profiles of the NtNPFs under N Stress and Co-Expression Network Analysis
2.7. 3D Protein Structure, Protein Transmembrane Domain, and Cis-Regulatory Element Analysis of the Hub NtNPFs in N. tabacum
2.8. The Physiological Responses of Two Cigar Tobaccos to N Stress
2.9. The Identification of Key Genes in Nitrate Efficient Uptake and Utilization in Cigar Tobacco
3. Discussion
3.1. Composition of the NPF Gene Family in Tobacco
3.2. Evolutionary and Structural Characteristics of the Tobacco NPF Family Genes
3.3. NtNPF Genes Function Critically in Nitrogen Uptake and Utilization of Cigar Tobacco
4. Materials and Methods
4.1. Identification of NPF Family Genes in Tobacco
4.2. Gene Structure, Conserved Motif, and Phylogenetic Analysis
4.3. Protein Biophysical Property and Evolutionary Pressure Analysis
4.4. Synteny Analysis and Chromosome Localization
4.5. Cis-Regulatory Elements Analysis
4.6. Heatmap and Co-Expression Network Analysis
4.7. Prediction of 3D Protein Structure
4.8. Plant Materials and Stress Treatments
4.9. Phenotypic Measurements
4.10. Gene Expression Analysis
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Guo, H.; He, X.; Zhang, H.; Tan, R.; Yang, J.; Xu, F.; Wang, S.; Yang, C.; Ding, G. Physiological Responses of Cigar Tobacco Crop to Nitrogen Deficiency and Genome-Wide Characterization of the NtNPF Family Genes. Plants 2022, 11, 3064. https://doi.org/10.3390/plants11223064
Guo H, He X, Zhang H, Tan R, Yang J, Xu F, Wang S, Yang C, Ding G. Physiological Responses of Cigar Tobacco Crop to Nitrogen Deficiency and Genome-Wide Characterization of the NtNPF Family Genes. Plants. 2022; 11(22):3064. https://doi.org/10.3390/plants11223064
Chicago/Turabian StyleGuo, Hao, Xuyou He, Hao Zhang, Ronglei Tan, Jinpeng Yang, Fangsen Xu, Sheliang Wang, Chunlei Yang, and Guangda Ding. 2022. "Physiological Responses of Cigar Tobacco Crop to Nitrogen Deficiency and Genome-Wide Characterization of the NtNPF Family Genes" Plants 11, no. 22: 3064. https://doi.org/10.3390/plants11223064