Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI
Abstract
:1. Introduction
2. Results
2.1. Yeast 2-Hybrid Library Screening
2.2. PiRhoGDI1 Interacts with and Is Phosphorylated by PiCDPK1 In Vitro
2.3. Confirmation of the Interaction of PiRhoGDI1 with ROP GTPase
2.4. PiCDPK1 Suppresses the Effect of PiRhoGDI1 Over-Expression In Vivo
2.5. Co-Expression with PiRhoGDI1 Rescues the PiCDPK1 Overexpression Phenotype
3. Discussion
4. Materials and Methods
4.1. Yeast 2-Hybrid Library Screening
4.2. Expression of Recombinant Proteins
4.3. Pull-Down Assay
4.4. Phosphorylation Assay
4.5. Stable Expression in Tobacco
4.6. Analysis of Transformed Pollen Tubes
4.7. Quantitative RT-PCR
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Scheible, N.; Yoon, G.M.; McCubbin, A.G. Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI. Plants 2022, 11, 254. https://doi.org/10.3390/plants11030254
Scheible N, Yoon GM, McCubbin AG. Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI. Plants. 2022; 11(3):254. https://doi.org/10.3390/plants11030254
Chicago/Turabian StyleScheible, Nolan, Gyeong Mee Yoon, and Andrew G. McCubbin. 2022. "Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI" Plants 11, no. 3: 254. https://doi.org/10.3390/plants11030254