Transcriptome Analysis Revealed a Positive Role of Ethephon on Chlorophyll Metabolism of Zoysia japonica under Cold Stress
Abstract
:1. Introduction
2. Results
2.1. Chlorophyll Content in Leaves
2.2. Sequence Assembly
2.3. Global Gene Expression Analysis
2.4. qRT-PCR Confirmation
2.5. Differential Gene Expression Related to Photosynthesis-Antenna Proteins and Porphyrin and Chlorophyll Metabolism
3. Discussion
3.1. Gene Expression Analysis of Porphyrin and Chl Metabolism
3.2. Effect of Ethephon Application on Chl Content under Cold Stress
3.3. Gene Expression Analysis of Photosynthesis-Antenna Proteins
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Determination of Chl Content in Leaves
4.3. Total RNA Extraction, RNA-Seq Library Construction, and RNA-Seq
4.4. RNA-Seq Data Processing and Assembly
4.5. DEG Analysis
4.6. Annotation Analysis
4.7. qRT-PCR Confirmation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Raw Reads | Total Reads | Clean Reads | Clean Bases | Q30 | GC Content | Total Map |
---|---|---|---|---|---|---|---|
(Gbp) | (%) | (%) | (%) | ||||
CE1 1 | 51696468 | 50530932 | 50530932 | 7.58 | 90.95 | 51.3 | 91.45 |
CE2 | 67066970 | 65714084 | 65714084 | 9.86 | 92.08 | 51.23 | 92.35 |
CE3 | 65392538 | 64083968 | 64083968 | 9.61 | 92.1 | 52.98 | 93.06 |
CS1 2 | 61806546 | 60439330 | 60439330 | 9.07 | 90.91 | 53.48 | 92.11 |
CS2 | 59147316 | 58002528 | 58002528 | 8.7 | 90.51 | 53.46 | 92.19 |
CS3 | 61224860 | 60038194 | 60038194 | 9.01 | 91.67 | 52.75 | 92.79 |
NT1 3 | 50204448 | 48478352 | 48478352 | 7.27 | 92.51 | 49.73 | 88.08 |
NT2 | 46169382 | 44677484 | 44677484 | 6.7 | 92.1 | 50.06 | 88.44 |
NT3 | 49753502 | 48029758 | 48029758 | 7.2 | 92.21 | 49.75 | 87.66 |
Gene ID | Primer Sequence |
---|---|
Actin | F:5′-GGTGTTATGGTTGGGATGG-3′ |
R:5′-CAGTGAGCAGGACAGGGTG-3′ | |
Zjn_sc00026.1.g00600.1.am.mkhc | F:5′-GCAGCAAGAACGAATGAT-3′ |
R:5′-CTGAAGAGTGGAAGGAGAA-3′ | |
Zjn_sc00014.1.g01180.1.sm.mkhc | F: GATGACAGAGATGCCAAT |
R: CGATGAATACACCAGACA | |
Zjn_sc00016.1.g04050.1.sm.mkhc | F: GGCAAGTGGTATTAGTGAA |
R: CAGTATGTGTTCCGTTGT | |
Zjn_sc00007.1.g00600.1.am.mkhc | F:GGACCTTGGACAGCATCTT |
R:CGGCGACGAAGTAGAGAAT | |
Zjn_sc00086.1.g01640.1.am.mk | F:CACGGACCAAGGACTCAAG |
R:CCAGCGTCAGTCACAAGA | |
Zjn_sc00133.1.g00260.1.am.mkhc | F:5′-GAAGGACACAGGAGTTGATG-3′ |
R:5′-CCATTACCAAGGCGTCTC-3′ | |
Zjn_sc00003.1.g01070.1.sm.mkhc | F:5′-ATCCTTACACCACTTCCT-3′ |
R:5′-CTCATCTCGCAACACATT-3′ | |
Zjn_sc00097.1.g01050.1.am.mk | F:5′-CTACCACGCTCAATCCTAT-3′ |
R:5′-GTCATCCTCCTCTTCATCTT-3′ | |
Zjn_sc00017.1.g04240.1.sm.mkhc | F: GGTGGTCATTGTGGATAA |
R: GGAGTCAGGTTCAGATAAG | |
Zjn_sc00016.1.g04940.1.am.mkhc | F: GCAAGAATGGAACCTGTG |
R: TCAGCAGCAATCTCATCA |
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Zhang, J.; Zhang, Z.; Liu, W.; Li, L.; Han, L.; Xu, L.; Zhao, Y. Transcriptome Analysis Revealed a Positive Role of Ethephon on Chlorophyll Metabolism of Zoysia japonica under Cold Stress. Plants 2022, 11, 442. https://doi.org/10.3390/plants11030442
Zhang J, Zhang Z, Liu W, Li L, Han L, Xu L, Zhao Y. Transcriptome Analysis Revealed a Positive Role of Ethephon on Chlorophyll Metabolism of Zoysia japonica under Cold Stress. Plants. 2022; 11(3):442. https://doi.org/10.3390/plants11030442
Chicago/Turabian StyleZhang, Jiahang, Zhiwei Zhang, Wen Liu, Lijing Li, Liebao Han, Lixin Xu, and Yuhong Zhao. 2022. "Transcriptome Analysis Revealed a Positive Role of Ethephon on Chlorophyll Metabolism of Zoysia japonica under Cold Stress" Plants 11, no. 3: 442. https://doi.org/10.3390/plants11030442