A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil
Abstract
:1. Introduction
2. Results
2.1. Population Screening
2.2. Whole-Plant Dose–Response Results
2.3. Screening for ALS Inhibitors
2.4. Inhibition of Cytochrome P-450 with Malathion
2.5. ALS Sequencing
3. Discussion
4. Materials and Methods
4.1. Population Screening
4.2. Whole-Plant Dose-Response Relationship
4.3. Screening for the Cross-Resistance for ALS Inhibitors
4.4. Inhibition of Cytochrome P-450 with Malathion
4.5. DNA Extraction, Amplification, and ALS Gene Sequencing
4.6. Data Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Herbicide (Dose in g ha−1) | Pre-Emergence Injury (%) | |||
---|---|---|---|---|
R | S | |||
Non-treated | 0 | c | 0 | b |
Diclosulam (25.2) | 65 | a | 100 | a |
Chlorimuron-ethyl (20) | 31.2 | b | 100 | a |
Metsulfuron-methyl (2) | 71.2 | a | 99 | a |
Imazethapyr (106) | 38.7 | b | 100 | a |
Herbicide | Post-Emergence Injury (%) | |||
R | S | |||
Non-treated | 0 | e | 0 | e |
Cloransulam-methyl (33.6) | 61.2 | bc | 99.5 | a |
Chlorimuron-ethyl (20) | 66.2 | ab | 95.5 | a |
Metsulfuron-methyl (2) | 51.2 | d | 98.2 | a |
Trifloxysulfuron-sodium (7.5) | 65 | b | 90 | ab |
Imazethapyr (106) | 52.5 | cd | 68.7 | c |
Imazapic (98) | 75 | a | 83.7 | b |
Pyrithiobac-sodium (36) | 5 | e | 51.2 | d |
Herbicide (Dose in g ha−1) | R | S | ||||||
---|---|---|---|---|---|---|---|---|
Injury (%) | Dry Mass * | Injury (%) | Dry Mass * | |||||
Non-treated | 0 | f | 100 | a | 0 | c | 100 | a |
Malathion (1000) | 0 | f | 84.5 | b | 0 | c | 108.5 | a |
Cloransulam-methyl (33.6) | 22.5 | e | 73.8 | bc | 99.5 | a | 0 | c |
Cloransulam-methyl (33.6) + | 37.5 | d | 58.8 | cd | 99.5 | a | 0 | c |
Chlorimuron-ethyl (20) | 47.5 | bc | 69.9 | bc | 99.5 | a | 0 | c |
Chlorimuron-ethyl (20) + | 42.5 | cd | 60.1 | c | 98.2 | a | 0 | c |
Imazethapyr (106) | 48.7 | b | 53.4 | de | 62.5 | b | 41.8 | b |
Imazethapyr (106)+ | 56.2 | a | 42.3 | e | 61.2 | b | 33.9 | b |
Population Code | City | State | Geographical Coordinates | |
---|---|---|---|---|
Latitude | Longitude | |||
1 | Engenheiro Beltrão | PR | 23°50′23″ S | 52°22′49″ W |
2 | Campina da Lagoa | PR | 24°55′17″ S | 53°21′35″ W |
3 | Medianeira | PR | 25°17′22″ S | 54°02′50″ W |
4 | Campo Bonito | PR | 25°02′02″ S | 54°01′42″ W |
5 | Quedas do Iguaçu | PR | 25°25′48″ S | 52°56′15″ W |
6 | Peabiru | PR | 23°56′31″ S | 52°20′43″ W |
7 | Campo Mourão | PR | 23°53′35″ S | 52°21′38″ W |
8 | Juranda | PR | 24°21′07″ S | 52°53′38″ W |
9 | Palotina | PR | 24°17′26″ S | 53°41′12″ W |
10 | Corbélia | PR | 24°51′32″ S | 53°19′4″ W |
11 | Cascavel | PR | 23°53′59″ S | 53°28′9″ W |
12 | Floresta | PR | 23°36′14″ S | 52°3′21″ W |
13 | Maringá | PR | 23°19′26″ S | 51°57′6″ W |
14 | Bandeirantes | PR | 123°5′16″ S | 50°26′20″ W |
15 | São Jorge do Ivaí | PR | 23°29′57″ S | 52°19′14″ W |
16 | Goioerê | PR | 24°05′23″ S | 53°07′01″ W |
17 | Maripá | PR | 24°30′25″ S | 53°43′12” W |
18 | Toledo | PR | 24°24′14″ S | 53°51′29” W |
19 | Londrina | PR | 23°10′56″ S | 51°10′57” W |
20 | Cambé | PR | 23°13′52″ S | 51°14′47″ W |
21 | Nova Aurora | PR | 24°23′51″ S | 53°21′55” W |
22 | Assis Chateaubriand | PR | 24°17′34″ S | 53°33′56″ W |
23 | Ventania | PR | 24°22′18″ S | 50°9′42″ W |
24 | Piraí do Sul | PR | 24°32′28″ S | 49°59′56″ W |
25 | Ivaiporã | PR | 24°24′2″ S | 51°47′23″ W |
26 | Pitanga | PR | 24°43′22″ S | 51°45′57″ W |
27 | Tibagi | PR | 24°33′24″ S | 50°23′37″ W |
28 | Castro | PR | 24°36′4″ S | 49°42′58″ W |
29 | Guarapuava | PR | 25°24′42″ S | 51°39′52″ W |
30 | Roncador | PR | 24°32′49″ S | 52°23′29″ W |
Treatments Exp 1 (Pre-Emergence) | Chemical Group | Treatments Exp 2 (Post-Emergence) | Chemical Group |
---|---|---|---|
Non-treated | - | Non treated | - |
Diclosulam (25.2) | TRI | Cloransulam-methyl 1/ (33.6) | TRI |
Chlorimuron-ethyl (20) | SUL | Chlorimuron-ethyl 2/ (20) | SUL |
Metsulfuron-methyl (2) | SUL | Metsulfuron-methyl 2/ (2) | SUL |
Imazethapyr (106) | IMI | Trifloxysulfuron-sodium (7.5) | SUL |
Imazethapyr (106) | IMI | ||
Imazapic (98) | IMI | ||
Pyrithiobac-sodium (36) | PYR |
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Vital Silva, V.; Mendes, R.; Suzukawa, A.; Adegas, F.; Marcelino-Guimaraes, F.; Oliveira, R., Jr. A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil. Plants 2022, 11, 467. https://doi.org/10.3390/plants11040467
Vital Silva V, Mendes R, Suzukawa A, Adegas F, Marcelino-Guimaraes F, Oliveira R Jr. A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil. Plants. 2022; 11(4):467. https://doi.org/10.3390/plants11040467
Chicago/Turabian StyleVital Silva, Vanessa, Rafael Mendes, Andreia Suzukawa, Fernando Adegas, Francismar Marcelino-Guimaraes, and Rubem Oliveira, Jr. 2022. "A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil" Plants 11, no. 4: 467. https://doi.org/10.3390/plants11040467