Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean
Abstract
:1. Introduction
2. Results
2.1. Bacterial Strains
2.2. Isolation and General Properties of Pseudomonas savastanoi pv. glycinea Bacteriophages
2.3. Phage P421 Genomic and Phylogenetic Characterisation
2.4. Phage Efficacy against Psg Leaf Infection
2.5. Phage Efficacy against Psg Seed Infection
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. Bacteriophage Isolation, Propagation and Purification
4.3. Electron Microscopy
4.4. Determination of Phage Host Specificity
4.5. Phage Adsorption and One-Step Growth Experiments
4.6. Phage Stability under Different Conditions
4.7. Genome Sequencing and Annotation
4.8. Phylogeny and Taxonomy Studies
4.9. Phage Control of Bacterial Blight on Soybean
4.9.1. Artificial Latent Infection of Soybean Seeds
4.9.2. Phage Application on Seeds
4.9.3. Artificial Infection of Soybean Leaves
4.9.4. Phage Application on Leaves
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tarakanov, R.I.; Lukianova, A.A.; Evseev, P.V.; Toshchakov, S.V.; Kulikov, E.E.; Ignatov, A.N.; Miroshnikov, K.A.; Dzhalilov, F.S.-U. Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean. Plants 2022, 11, 938. https://doi.org/10.3390/plants11070938
Tarakanov RI, Lukianova AA, Evseev PV, Toshchakov SV, Kulikov EE, Ignatov AN, Miroshnikov KA, Dzhalilov FS-U. Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean. Plants. 2022; 11(7):938. https://doi.org/10.3390/plants11070938
Chicago/Turabian StyleTarakanov, Rashit I., Anna A. Lukianova, Peter V. Evseev, Stepan V. Toshchakov, Eugene E. Kulikov, Alexander N. Ignatov, Konstantin A. Miroshnikov, and Fevzi S.-U. Dzhalilov. 2022. "Bacteriophage Control of Pseudomonas savastanoi pv. glycinea in Soybean" Plants 11, no. 7: 938. https://doi.org/10.3390/plants11070938