Exogenous Selenium and Biochar Application Modulate the Growth and Selenium Uptake of Medicinal Legume Astragalus Species
Abstract
:1. Introduction
2. Results
2.1. Comparison of Growth Indexes of Astragalus Species in Different Se Concentrations
2.2. Comparison of Se Content of Astragalus Species in Different Se Concentrations
2.2.1. Effects of Biochar Application on Se Content in ABH Roots
2.2.2. Effects of Biochar Application on Se Content in ABH Above-Ground
2.3. Comparison of Se Content in the Roots of ABH and AB
2.4. Comparison of Se Content in the above Ground of ABH and AB
2.4.1. Effects of Biochar Application on Total Flavonoid Content in ABH Roots
2.4.2. Effects of Biochar Application on Total Flavonoid Content in ABH Roots
2.4.3. Effects of Biochar on Calycosin-7-glucoside Content in ABH Roots
2.4.4. Effects of Different Treatments on the Accumulation and Transport of Se in Astragalus Species
2.5. Correlation Analysis between Se and Growth Indexes and Active Components in ABH
3. Discussion
4. Materials and Methods
4.1. Plant Material and Soil Physicochemical Property Measurement
4.2. Experimental Design
4.3. Determination of Plant Growth Indexes
4.4. Determination of Active Components and Se Content
4.5. Data Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group Information | Se Concentration (mg/kg) | Enrichment Coefficient in Roots | Enrichment Coefficient in Above-Ground | Transport Coefficient |
---|---|---|---|---|
ABH | 0 | 2.463 ± 0.187 h | 2.158 ± 0.238 i | 0.875 ± 0.041 b |
3 | 6.744 ± 0.334 f | 5.124 ± 0.145 d | 0.757 ± 0.031 c | |
6 | 9.169 ± 1.032 e | 5.158 ± 0.215 d | 0.565 ± 0.018 g | |
9 | 6.522 ± 0.524 f | 4.349 ± 0.032 ef | 0.668 ± 0.010 e | |
12 | 5.872 ± 0.516f g | 4.176 ± 0.064 f | 0.714 ± 0.037 d | |
ABH with biochar | 0 | 8.414 ± 1.602 e | 1.807 ± 0.263 d | 0.620 ± 0.022 f |
3 | 12.323 ± 1.042 d | 6.446 ± 0.583 c | 0.524 ± 0.045 gh | |
6 | 19.468 ± 2.557 a | 8.516 ± 0.302 a | 0.440 ± 0.017 i | |
9 | 16.653 ± 1.592 b | 8.768 ± 0.251 a | 0.529 ± 0.031 gh | |
12 | 14.708 ± 1.390 c | 7.455 ± 0.665 b | 0.509 ± 0.054 h | |
AB | 0 | 1.934 ± 0.190 h | 2.896 ± 0.232 h | 1.500 ± 0.046 a |
3 | 4.752 ± 0.995 g | 3.633 ± 0.456 g | 0.774 ± 0.027 c | |
6 | 4.420 ± 0.159 g | 3.397 ± 0.084 e | 0.769 ± 0.032 c | |
9 | 9.980 ± 0.113 d | 4.523 ± 0.111 ef | 0.453 ± 0.009 j | |
12 | 10.756 ± 0.400 d | 4.847 ± 0.294 de | 0.451 ± 0.031 i |
Components | Se Content in Roots | Se Content in Above-Ground | Plant Height | Taproot Length | Total Flavonoid | Total Polysaccharide | Calycosin-7-Glucoside |
---|---|---|---|---|---|---|---|
Se content in roots | 1.000 | 0.955 ** | 0.682 ** | 0.517 ** | −0.152 | 0.367 | 0.775 ** |
Se content in above-ground | 1.000 | 0.581 ** | 0.352 | −0.286 | 0.193 | 0.659 ** | |
Taproot length | 1.000 | 0.769 ** | 0.101 | 0.694 ** | 0.653 ** | ||
Plant height | 1.000 | 0.105 | 0.901 ** | 0.685 ** | |||
Total flavonoid | 1.000 | 0.224 | −0.051 | ||||
Total polysaccharide | 1.000 | 0.626 ** | |||||
Calycosin-7-glucoside | 1.000 |
Se Concentration (mg/kg) | ABH | ABH with Biochar | AB |
---|---|---|---|
0 | GCK | GTCK | JCK |
3 | GXA | GTXA | JXA |
6 | GXB | GTXB | JXB |
9 | GXC | GTXC | JXC |
12 | GXD | GTXD | JXD |
Component | Linear Relationship | R2 | Linear Range |
---|---|---|---|
Total flavone | Y1 = 71.415X1 + 0.0178 | 0.9998 | 2.56–25.60 μg/mL |
Polysaccharide | Y2 = 10.73X2 − 0.065 | 0.9991 | 20.00–100.00 μg/mL |
Calycosin-7-glucoside | Y3 = 1,651,116.4080X3 − 2740.1121 | 0.9999 | 0.04–0.60 μg |
Se | Y4 = 167.63X4 + 0.0007 | 0.9999 | 1.00–20.00 μg/L |
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Ma, S.; Zhu, G.; Parhat, R.; Jin, Y.; Wang, X.; Wu, W.; Xu, W.; Wang, Y.; Chen, W. Exogenous Selenium and Biochar Application Modulate the Growth and Selenium Uptake of Medicinal Legume Astragalus Species. Plants 2023, 12, 1957. https://doi.org/10.3390/plants12101957
Ma S, Zhu G, Parhat R, Jin Y, Wang X, Wu W, Xu W, Wang Y, Chen W. Exogenous Selenium and Biochar Application Modulate the Growth and Selenium Uptake of Medicinal Legume Astragalus Species. Plants. 2023; 12(10):1957. https://doi.org/10.3390/plants12101957
Chicago/Turabian StyleMa, Shengjun, Guangwei Zhu, Rozi Parhat, Yuanyuan Jin, Xueshuang Wang, Wenping Wu, Wanli Xu, Yanling Wang, and Wenfeng Chen. 2023. "Exogenous Selenium and Biochar Application Modulate the Growth and Selenium Uptake of Medicinal Legume Astragalus Species" Plants 12, no. 10: 1957. https://doi.org/10.3390/plants12101957