Identification of High Tolerance to Jujube Witches’ Broom in Indian Jujube (Ziziphus mauritiana Lam.) and Mining Differentially Expressed Genes Related to the Tolerance through Transcriptome Analysis
Abstract
:1. Introduction
2. Results
2.1. Indian Jujube ‘Cuimi’ Is High-Tolerant to JWB
2.2. Screening for Differentially Expressed Genes in ‘Cuimi’ and ‘Huping’
2.3. Gene Ontology (GO) Enrichment Analysis of DEGs
2.4. Identification of Genes Associated with ‘Cuimi’ High Tolerance
3. Discussion
3.1. The High Tolerance to JWB of ‘Cuimi’
3.2. Response of JWB Susceptible Jujube Cultivar to Phytoplasma Infection
3.3. Response of JWB Tolerant Jujube Cultivar to Phytoplasma Infection
4. Materials and Methods
4.1. Plant Materials
4.2. Detection of JWB Phytoplasma Using Nested PCR
4.3. Quantitative PCR (qPCR)
4.4. RNA-Seq Library Construction and Sequencing
4.5. Data Analysis
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xu, Y.; Wang, C.; Kong, D.; Cao, M.; Zhang, Q.; Tahir, M.; Yang, Y.; Yang, S.; Bo, W.; Pang, X. Identification of High Tolerance to Jujube Witches’ Broom in Indian Jujube (Ziziphus mauritiana Lam.) and Mining Differentially Expressed Genes Related to the Tolerance through Transcriptome Analysis. Plants 2023, 12, 2082. https://doi.org/10.3390/plants12112082
Xu Y, Wang C, Kong D, Cao M, Zhang Q, Tahir M, Yang Y, Yang S, Bo W, Pang X. Identification of High Tolerance to Jujube Witches’ Broom in Indian Jujube (Ziziphus mauritiana Lam.) and Mining Differentially Expressed Genes Related to the Tolerance through Transcriptome Analysis. Plants. 2023; 12(11):2082. https://doi.org/10.3390/plants12112082
Chicago/Turabian StyleXu, Yaru, Chao Wang, Decang Kong, Ming Cao, Qiong Zhang, Muhammad Tahir, Ying Yang, Shuang Yang, Wenhao Bo, and Xiaoming Pang. 2023. "Identification of High Tolerance to Jujube Witches’ Broom in Indian Jujube (Ziziphus mauritiana Lam.) and Mining Differentially Expressed Genes Related to the Tolerance through Transcriptome Analysis" Plants 12, no. 11: 2082. https://doi.org/10.3390/plants12112082