Mutations in Selected ABA-Related Genes Reduce Level of Arabidopsis thaliana Susceptibility to the Beet Cyst Nematode Heterodera schachtii
Abstract
:1. Introduction
2. Results
2.1. Analysis of Changes in A. thaliana Gene Expression at Early Stage of Nematode Parasitism
2.2. Endogenous ABA Concentrations in Arabidopsis Mutants
2.3. Susceptibility Level of Arabidopsis Mutants to H. schachtii
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Nematode Culture, Infection Assays and Measurements
4.3. Endogenous ABA Concentration
4.4. RNA Isolation and cDNA Synthesis
4.5. Quantitative Real-Time PCR
4.6. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene Acc. No. | Mutant NASC Stock No./Polymorphism | Gene Product | Gene Function | Mutant Phenotype |
---|---|---|---|---|---|
ABI1 (ABA-insensitive1) | AT4G26080 | SALK_072009 /abi1-2 | 2C protein phosphatase | ABA signalling | [23] |
ABI2 (ABA-insensitive2) | AT5G57050 | SAIL_547_C10 | 2C protein phosphatase | nr * | |
ABI5 (ABA-insensitive5) | AT2G36270 | SALK_013163 /abi5-2 | transcription factor | [24] | |
PYL5 (PYR1-like5) | AT5G05440 | SM_3_532 | Receptor | nr * | |
PYL6 (PYR1-like6) | AT2G40330 | SAIL_1179_D01 | Receptor | [25] | |
CYP707A1 | AT4G19230 | SALK_069127 /CYP707A1-1 | cytochrome P450 monooxygenase | ABA catabolism | [26] |
CYP707A4 | AT3G19270 | SALK_201208C | cytochrome P450 monooxygenase | nr * |
Gene Name | Gene Acc. No. | Primers 5′-3′ | Used for |
---|---|---|---|
ABI1 | AT4G26080 | TGAATATAGGAAGTCTGAAGCAAGTG CGAAACAGCATCTTCCATCTC | Genotyping |
ATGTCGAGATCCATTGGCGAT TTGCCATCTCACACGCTTCT | Real-time PCR | ||
ABI2 | AT5G57050 | TTCCTTCTCCTCTTTTCTCCG TTGATCCGAGATCGATGAATC | Genotyping |
CGACTCTAGGGCGGTTTTGT AGGTATCTATCGCCAATGGATCT | Real-time PCR | ||
ABI5 | AT2G36270 | CAATGGAAGTTCGGAATCATG CACTCGTTTTCTTCTTAAAGCG | Genotyping |
AGTGGATGGTCCAGTGGAGA CAACTCCGCCAATGCATGTT | Real-time PCR | ||
CYP707A1 | AT4G19230 | CATGAACGTATTGGGTTTTGG TCCTGATATTGAATCCATCGC | Genotyping |
AGAAGCTGTCGAAGATGTCGAA GGGTTTTGGAGCCACCTCG | Real-time PCR | ||
CYP707A4 | AT3G19270 | TCGATCATTTACAACATAAG GGAAGACCGTTTTGAGGTAT | Genotyping |
AAGGTCGTGTGCTAACCCAA AGCCTTTTGCTCAGCCTTAAC | Real-time PCR | ||
PYL5 | AT5G05440 | TGGACACAAGGATCAACCAAC CAGCGAACAAGATCAAAAAGC | Genotyping |
CCGTGGTGGTGGAGTCTTAC AGAGACTGAAGGTTGCACCG | Real-time PCR | ||
PYL6 | AT2G40330 | GCCTCGAGACAGTAGAAGATTG CGTATGACTCAACGACACGTG | Genotyping |
TATCGGAGACGGTCGAGAGG ACCAACGACGCTGAAACTGA | Real-time PCR | ||
UBP22 | AT5G10790 | CACAAGGGGATGTTGGAATCAG ACTCACATCCTCTCACCACTTC | Real-time PCR |
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Różańska, E.; Krępski, T.; Wiśniewska, A. Mutations in Selected ABA-Related Genes Reduce Level of Arabidopsis thaliana Susceptibility to the Beet Cyst Nematode Heterodera schachtii. Plants 2023, 12, 2299. https://doi.org/10.3390/plants12122299
Różańska E, Krępski T, Wiśniewska A. Mutations in Selected ABA-Related Genes Reduce Level of Arabidopsis thaliana Susceptibility to the Beet Cyst Nematode Heterodera schachtii. Plants. 2023; 12(12):2299. https://doi.org/10.3390/plants12122299
Chicago/Turabian StyleRóżańska, Elżbieta, Tomasz Krępski, and Anita Wiśniewska. 2023. "Mutations in Selected ABA-Related Genes Reduce Level of Arabidopsis thaliana Susceptibility to the Beet Cyst Nematode Heterodera schachtii" Plants 12, no. 12: 2299. https://doi.org/10.3390/plants12122299