Comprehensive Investigation of Die-Back Disease Caused by Fusarium in Durian
Abstract
:1. Introduction
2. Results
2.1. Fungal Isolation and Purification
2.2. DNA Marker and Fungal Identification
2.3. Morphological Characteristics
2.4. Pathogenicity Test
2.5. Fusarium–Ambrosia Beetle Association
3. Discussion
4. Materials and Methods
4.1. Fungal Isolation and Purification
4.2. DNA Extraction and Molecular Identification
4.3. Morphological Characteristics
4.4. Phylogenetic Analysis
4.5. Pathogenicity Test
4.6. Fusarium–Ambrosia Beetle Association
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Collection 1 | GenBank Accession Numbers | Identification (GenBank References at 99–100% Similarity) | ||
---|---|---|---|---|---|
ITS | TEF1-α | RPB2 | |||
Fusarium Group 1 | DCHA704 | LC745744.1 | LC745761.1 | LC745778.1 | F. incarnatum (NRRL32867, NRRL32866) |
Fusarium Group 2 | DCHA1301 | LC745745.1 | LC745762.1 | LC745779.1 | F. incarnatum (NRRL32867, NRRL32866) |
Fusarium Group 3 | DCHA1801 | LC745746.1 | LC745763.1 | LC745780.1 | F. incarnatum (NRRL32867, NRRL32866) |
Fusarium Group 4 | DCHA408 | LC745747.1 | LC745764.1 | LC745781.1 | F. solani (NRRL32810, NRRL32791) |
Fusarium Group 5 | DCHU303 | LC745748.1 | LC745765.1 | LC745782.1 | F. solani NRRL32741 |
Fusarium Group 6 | DCHA1607 | LC745749.1 | LC745766.1 | LC745783.1 | F. incarnatum (NRRL32867, NRRL32866) |
Fusarium Group 7 | DCHU107 | LC745750.1 | LC745767.1 | LC745784.1 | F. solani (NRRL32741) |
Fusarium Group 8 | DCHA902 | LC745751.1 | LC745768.1 | LC745785.1 | F. solani (NRRL32810, NRRL32791) |
Fusarium Group 9 | DCHU801 | LC745752.1 | LC745769.1 | LC745786.1 | F. solani (NRRL32741) |
Fusarium Group 10 | DCHA1708 | LC745753.1 | LC745770.1 | LC745787.1 | F. solani (NRRL32741) |
Fusarium Group 11 | DCHA1101 | LC745754.1 | LC745771.1 | LC745788.1 | F. incarnatum (NRRL32867, NRRL32866) |
Fusarium Group 12 | DCHA805 | LC745755.1 | LC745772.1 | LC745789.1 | F. mangiferae (CBS119853) |
Fusarium Group 13 | DCHU403 | LC745756.1 | LC745773.1 | LC745790.1 | F. solani (NRRL32741) |
Fusarium Group 14 | DCHU1404 | LC745757.1 | LC745774.1 | LC745791.1 | F. solani (NRRL32741) |
Fusarium Group 15 | DCHA102 | LC745758.1 | LC745775.1 | LC745792.1 | F. solani (NRRL32810, NRRL32791) |
Fusarium Group 16 | DCHA904 | LC745759.1 | LC745776.1 | LC745793.1 | F. solani (NRRL32741) |
Fusarium Group 17 | DCHA424 | LC745760.1 | LC745777.1 | LC745794.1 | F. solani (NRRL32810, NRRL32791) |
Group | Species | Representative Isolate | Mean Lesion Size (cm) ± SD |
---|---|---|---|
1 | F. incarnatum | DCHA704 | 3.35 ± 0.32 e |
2 | F. incarnatum | DCHA1301 | 2.38 ± 0.34 f |
3 | F. incarnatum | DCHA1801 | 1.76 ± 0.14 g |
4 | F. solani | DCHA408 | 3.49 ± 0.27 e |
5 | F. solani | DCHU303 | 3.94 ± 0.13 d |
6 | F. incarnatum | DCHA1607 | 2.42 ± 0.11 f |
7 | F. solani | DCHU107 | 5.05 ± 0.26 c |
8 | F. solani | DCHA902 | 5.38 ± 0.19 b |
9 | F. solani | DCHU801 | 5.98 ± 0.08 a |
10 | F. solani | DCHA1708 | 5.90 ± 0.22 a |
11 | F. incarnatum | DCHA1101 | 3.98 ± 0.17 d |
12 | F. mangiferae | DCHA805 | 0.00 ± 0.00 h |
13 | F. solani | DCHU403 | 3.88 ± 0.17 d |
14 | F. solani | DCHU1404 | 3.60 ± 0.29 e |
15 | F. solani | DCHA102 | 4.06 ± 0.07 d |
16 | F. solani | DCHA904 | 3.94 ± 0.07 d |
17 | F. solani | DCHA424 | 4.05 ± 0.07 d |
F-test | *** | ||
C.V. (%) | 5.2863 | ||
MSE | 0.039 |
Gene/DNA Region | Primer Details | |||
---|---|---|---|---|
Name | Name | Sequence (5′→3′) | Ta (°C) | References |
ITS region | ITS5 | GGAAGTAAAAGTCGTAACAAGG | 56 | [45] |
ITS4 | TCCTCCGCTTATTGATATGC | |||
TEF1-α gene | EF1-F | ATGGGTAAGGARGACAAGAC | 56 | [46] |
EF2-R | GGARGTACCAGTSATCATGTT | |||
RPB2 gene | fRPB2-7cf | ATGGGYAARCAAGCYATGGG | 57.2 | [47] |
fRPB2-7cr | CCCATRGCTTGYTTRCCCAT |
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Pongpisutta, R.; Keawmanee, P.; Sanguansub, S.; Dokchan, P.; Bincader, S.; Phuntumart, V.; Rattanakreetakul, C. Comprehensive Investigation of Die-Back Disease Caused by Fusarium in Durian. Plants 2023, 12, 3045. https://doi.org/10.3390/plants12173045
Pongpisutta R, Keawmanee P, Sanguansub S, Dokchan P, Bincader S, Phuntumart V, Rattanakreetakul C. Comprehensive Investigation of Die-Back Disease Caused by Fusarium in Durian. Plants. 2023; 12(17):3045. https://doi.org/10.3390/plants12173045
Chicago/Turabian StylePongpisutta, Ratiya, Pisut Keawmanee, Sunisa Sanguansub, Paradorn Dokchan, Santiti Bincader, Vipaporn Phuntumart, and Chainarong Rattanakreetakul. 2023. "Comprehensive Investigation of Die-Back Disease Caused by Fusarium in Durian" Plants 12, no. 17: 3045. https://doi.org/10.3390/plants12173045