Potato Non-Specific Lipid Transfer Protein StnsLTPI.33 Is Associated with the Production of Reactive Oxygen Species, Plant Growth, and Susceptibility to Alternaria solani
Abstract
:1. Introduction
2. Results
2.1. The Gene PGSC0003DMG400031236 Encodes a Putative nsLTP
2.2. Gene Expression in Different Plant Tissues and in Response to Hormones
2.3. Subcellular Localization of StnsLTPI.33
2.4. Phenotypic Characterization of Transgenic Lines Overexpressing StnsLTPI.33
2.5. Response of StnsLTPI.33-Overexpression Lines to Salt Stress
2.6. Response of StnsLTPI.33-Overexpression Lines to PVY
2.7. Response of StnsLTPI.33-Overexpression Lines to Alternaria Solani
2.8. StnsLTPI.33-Overexpression Lines Accumulate Superoxide Anions
3. Discussion
4. Materials and Methods
4.1. Phylogenetic Analysis
4.2. Plant Growth Conditions
4.3. Generation of StnsLTPI.33-Overexpressing Potato Plants
4.4. Quantitative Reverse Transcription PCR
4.5. Hormone Treatments
4.6. Subcellular Localization
4.7. Detection of Hydrogen Peroxide and Superoxide Anion Radicals
4.8. Metabolite Analysis by GC-MS
4.9. PVY Strains, Inoculation and Detection
4.10. Alternaria Solani Disease Assays
4.11. Statistical Analysis of the Results
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Experiment 1 | Experiment 2 | Experiment 3 | |||||
---|---|---|---|---|---|---|---|
Treatment | Potato Line | In-Season a | Seedborne b | In-Season a | Seedborne b | In-Season a | Seedborne b |
Mock | PR | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 |
LTP10 | 0/6 | 0/5 | 1/6 | 0/5 | 0/3 | 0/2 | |
LTP11 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/4 | |
PVYO | PR | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 |
LTP10 | 0/6 | 0/6 | 0/6 | 0/4 | 1/4 | 0/2 | |
LTP11 | 0/5 | 0/5 | 0/5 | 0/5 | 2/5 | 0/5 | |
PVYN-Wilga | PR | 0/6 | 0/6 | 4/6 | 3/6 | 2/6 | 0/6 |
LTP10 | 0/4 | 0/4 | 2/5 | 4/5 | 0/4 | 0/4 | |
LTP11 | 0/6 | 0/5 | 0/6 | 4/6 | 1/6 | 0/5 | |
PVYNTN | PR | 4/6 | 4/6 | 5/6 | 5/6 | 5/6 | 5/6 |
LTP10 | 5/6 | 5/6 | 5/6 | 5/5 | 4/5 | 2/3 | |
LTP11 | 4/6 | 4/6 | 4/4 | 4/4 | 6/6 | 5/6 |
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Bvindi, C.; Howe, K.; Wang, Y.; Mullen, R.T.; Rogan, C.J.; Anderson, J.C.; Goyer, A. Potato Non-Specific Lipid Transfer Protein StnsLTPI.33 Is Associated with the Production of Reactive Oxygen Species, Plant Growth, and Susceptibility to Alternaria solani. Plants 2023, 12, 3129. https://doi.org/10.3390/plants12173129
Bvindi C, Howe K, Wang Y, Mullen RT, Rogan CJ, Anderson JC, Goyer A. Potato Non-Specific Lipid Transfer Protein StnsLTPI.33 Is Associated with the Production of Reactive Oxygen Species, Plant Growth, and Susceptibility to Alternaria solani. Plants. 2023; 12(17):3129. https://doi.org/10.3390/plants12173129
Chicago/Turabian StyleBvindi, Carol, Kate Howe, You Wang, Robert T. Mullen, Conner J. Rogan, Jeffrey C. Anderson, and Aymeric Goyer. 2023. "Potato Non-Specific Lipid Transfer Protein StnsLTPI.33 Is Associated with the Production of Reactive Oxygen Species, Plant Growth, and Susceptibility to Alternaria solani" Plants 12, no. 17: 3129. https://doi.org/10.3390/plants12173129