Multi-Omics Revealed Peanut Root Metabolism Regulated by Exogenous Calcium under Salt Stress
Abstract
:1. Introduction
2. Results
2.1. Physiological Phenotypic Traits of Exogenous Calcium Alleviate Salt Stress in Peanut Seedling Roots
2.2. DEGs and DEMs Screening
2.3. KEGG Co-Enrichment Analysis of Differentially Expressed Genes and Differentially Expressed Metabolites
2.4. Comprehensive Analysis of DEMs and Antioxidant Enzyme DEGs
2.5. Comprehensive Analysis of Differentially Expressed Genes and Metabolites in Phytohormone Biosynthesis and Signal Transduction Pathways
2.6. RT-qPCR Verification
3. Discussion
4. Materials and Methods
4.1. Experimental Materials Cultivation and Experimental Design
4.2. Experimental Materials Cultivation and Experimental Design
4.2.1. Root Vitality
4.2.2. Na+, K+ and Ca2+ Contents
4.2.3. Superoxide Anion (O2−), Hydrogen Peroxide (H2O2) and Malondialdehyde (MDA) Content
4.2.4. Antioxidant Enzyme (SOD, CAT, POD) Activities
4.3. RNA Extraction, Library Construction, and RNA Sequencing
4.4. Broadly Targeted Metabolome Assay
4.5. Phytohormone Absolute Quantitation Assay
4.6. RT-qPCR Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Dong, X.; Gao, Y.; Bao, X.; Wang, R.; Ma, X.; Zhang, H.; Liu, Y.; Jin, L.; Lin, G. Multi-Omics Revealed Peanut Root Metabolism Regulated by Exogenous Calcium under Salt Stress. Plants 2023, 12, 3130. https://doi.org/10.3390/plants12173130
Dong X, Gao Y, Bao X, Wang R, Ma X, Zhang H, Liu Y, Jin L, Lin G. Multi-Omics Revealed Peanut Root Metabolism Regulated by Exogenous Calcium under Salt Stress. Plants. 2023; 12(17):3130. https://doi.org/10.3390/plants12173130
Chicago/Turabian StyleDong, Xuan, Yan Gao, Xuefeng Bao, Rongjin Wang, Xinyu Ma, Hui Zhang, Yifei Liu, Lanshu Jin, and Guolin Lin. 2023. "Multi-Omics Revealed Peanut Root Metabolism Regulated by Exogenous Calcium under Salt Stress" Plants 12, no. 17: 3130. https://doi.org/10.3390/plants12173130