Phosphate Transporter BnaPT37 Regulates Phosphate Homeostasis in Brassica napus by Changing Its Translocation and Distribution In Vivo
Abstract
:1. Introduction
2. Results
2.1. Sequence Alignment and Phylogenetic Analysis of BnaPT37 Gene
2.2. The Expression Pattern of BnaPT37 in Response to Different P Supplies
2.3. BnaPT37 Is P-Starvation-Induced and Plasma Membrane-Localized Protein
2.4. BnaPT37 Mediates Pi Transport in Yeast and in Arabidopsis
2.5. Overexpression of BnaPT37 Retards Rapeseed Growth at Seedling Stage
2.6. Pi Translocation and Distribution Changes in the BnaPT37 Transgenic Plants
2.7. Overexpression of BnaPT37 Affects Plant Height and P Distribution at the Flowering Stage
2.8. BnaPT37 Is Involved in the Translocation of P into Grains at the Ripening Stage
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Gene Structure and Sequence Analysis
4.3. RNA Extraction and Real-Time Quantitative PCR Analysis
4.4. Vector Construction and Transformation in Arabidopsis and B. napus
4.5. Subcellular Localization Analysis
4.6. Tissue Localization Analysis
4.7. Pi Transport Activity Assay in Yeast
4.8. Measurement of Inorganic P and Total P Concentrations in Plants
4.9. Xylem Sap Collection
4.10. Statistical Analysis of Data
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Li, Y.; Wang, X.; Zhang, H.; Ye, X.; Shi, L.; Xu, F.; Ding, G. Phosphate Transporter BnaPT37 Regulates Phosphate Homeostasis in Brassica napus by Changing Its Translocation and Distribution In Vivo. Plants 2023, 12, 3362. https://doi.org/10.3390/plants12193362
Li Y, Wang X, Zhang H, Ye X, Shi L, Xu F, Ding G. Phosphate Transporter BnaPT37 Regulates Phosphate Homeostasis in Brassica napus by Changing Its Translocation and Distribution In Vivo. Plants. 2023; 12(19):3362. https://doi.org/10.3390/plants12193362
Chicago/Turabian StyleLi, Yu, Xue Wang, Hao Zhang, Xiangsheng Ye, Lei Shi, Fangsen Xu, and Guangda Ding. 2023. "Phosphate Transporter BnaPT37 Regulates Phosphate Homeostasis in Brassica napus by Changing Its Translocation and Distribution In Vivo" Plants 12, no. 19: 3362. https://doi.org/10.3390/plants12193362