Genome-Wide Identification and Analysis of Catharanthus roseus Receptor-like Kinase 1-like Proteins in Eggplant
Abstract
:1. Introduction
2. Results
2.1. Identification of CrRLK1L Proteins in Eggplant
2.2. Phylogenetic Analysis of the SmCrRLK1Ls
2.3. Chromosome Distribution of SmCrRLK1Ls
2.4. Protein Domain and Gene Structure Analysis of SmCrRLK1L
2.5. Conserved Motifs and Subcellular Localization of SmCrRLK1L Prediction
2.6. SmCrRLK1L Gene Promoter Analysis
2.7. SmCrRLK1L Gene Duplication Events
2.8. Expression Analysis of SmCrRLK1L Genes in Response to Botrytis cinerea Infection
3. Discussion
4. Materials and Methods
4.1. SmCrRLK1L Members, Physicochemical Property Identification
4.2. Phylogenetic Analysis
4.3. Gene Location and Collinearity Analysis
4.4. Protein Domain and Gene Structure Analysis
4.5. Subcellular Localization Prediction
4.6. Protein Conserved Motifs Analysis
4.7. Promoter Analysis
4.8. Transcriptomic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Protein Name | SGN Locus | Protein Length | MW | Theoretical pI | Instability Index | Aliphatic Index | GRAVY |
---|---|---|---|---|---|---|---|
SmCrRLK1L1 | SMEL4.1_00g002980.1.01 | 916 | 102,981.91 | 9.05 | 38.81 | 89.29 | −0.244 |
SmCrRLK1L2 | SMEL4.1_00g011070.1.01 | 904 | 100,649.88 | 5.78 | 40.63 | 84.55 | −0.284 |
SmCrRLK1L3 | SMEL4.1_01g004620.1.01 | 878 | 96,435.51 | 6.03 | 42.89 | 84.54 | −0.164 |
SmCrRLK1L4 | SMEL4.1_01g027970.1.01 | 850 | 94,767.29 | 6.48 | 44.05 | 80.29 | −0.256 |
SmCrRLK1L5 | SMEL4.1_02g011520.1.01 | 990 | 111,146.47 | 8.55 | 35.88 | 89.65 | −0.252 |
SmCrRLK1L6 | SMEL4.1_02g011530.1.01 | 780 | 86,927.27 | 8.05 | 27.96 | 88.64 | −0.27 |
SmCrRLK1L7 | SMEL4.1_02g011560.1.01 | 956 | 106,793.65 | 5.85 | 36.2 | 90.76 | −0.232 |
SmCrRLK1L8 | SMEL4.1_02g011630.1.01 | 443 | 49,500.78 | 9.02 | 37.46 | 90.52 | −0.258 |
SmCrRLK1L9 | SMEL4.1_02g011650.1.01 | 930 | 103,516.67 | 7.27 | 35.74 | 92.49 | −0.151 |
SmCrRLK1L10 | SMEL4.1_02g013460.1.01 | 868 | 96,482.78 | 5.66 | 40.89 | 81.12 | −0.273 |
SmCrRLK1L11 | SMEL4.1_02g026370.1.01 | 1026 | 114,710.11 | 5.84 | 40.63 | 81.01 | −0.273 |
SmCrRLK1L12 | SMEL4.1_02g026380.1.01 | 997 | 112,040.66 | 6.38 | 41.89 | 84.84 | −0.259 |
SmCrRLK1L13 | SMEL4.1_03g004220.1.01 | 827 | 92,723.16 | 5.58 | 40.19 | 86.09 | −0.207 |
SmCrRLK1L14 | SMEL4.1_03g014350.1.01 | 610 | 67,859.65 | 5.85 | 31.72 | 96.05 | −0.06 |
SmCrRLK1L15 | SMEL4.1_03g027050.1.01 | 850 | 93,804.86 | 6.03 | 38.84 | 86.24 | −0.09 |
SmCrRLK1L16 | SMEL4.1_03g032580.1.01 | 834 | 93,418.44 | 5.78 | 32.53 | 94.21 | −0.14 |
SmCrRLK1L17 | SMEL4.1_04g002370.1.01 | 840 | 91,907.43 | 5.27 | 37.89 | 94.46 | 0.025 |
SmCrRLK1L18 | SMEL4.1_05g003720.1.01 | 820 | 90,378.18 | 6.53 | 37.67 | 89.37 | −0.065 |
SmCrRLK1L19 | SMEL4.1_05g004710.1.01 | 819 | 91,513.23 | 5.65 | 34.6 | 90.13 | −0.086 |
SmCrRLK1L20 | SMEL4.1_06g000710.1.01 | 866 | 95,410.66 | 6.42 | 38.33 | 80.25 | −0.298 |
SmCrRLK1L21 | SMEL4.1_06g000720.1.01 | 911 | 100,056.89 | 6.11 | 34.63 | 82.52 | −0.266 |
SmCrRLK1L22 | SMEL4.1_06g004680.1.01 | 829 | 90,489.55 | 5.8 | 36.94 | 85.11 | −0.077 |
SmCrRLK1L23 | SMEL4.1_07g012220.1.01 | 885 | 96,959.49 | 5.77 | 43.66 | 79.51 | −0.209 |
SmCrRLK1L24 | SMEL4.1_07g020590.1.01 | 983 | 108,300.31 | 8.38 | 30.1 | 94.31 | −0.108 |
SmCrRLK1L25 | SMEL4.1_07g023150.1.01 | 843 | 92,227.57 | 5.51 | 37.69 | 89.18 | −0.078 |
SmCrRLK1L26 | SMEL4.1_07g026240.1.01 | 1058 | 116,977.8 | 6.3 | 31.28 | 92.28 | −0.139 |
SmCrRLK1L27 | SMEL4.1_09g003310.1.01 | 928 | 102,821.64 | 5.61 | 35.6 | 90.15 | −0.181 |
SmCrRLK1L28 | SMEL4.1_09g010930.1.01 | 888 | 97,206.73 | 5.69 | 42.59 | 80.01 | −0.207 |
SmCrRLK1L29 | SMEL4.1_09g012150.1.01 | 1084 | 120,775.58 | 5.77 | 46.93 | 97.1 | −0.09 |
SmCrRLK1L30 | SMEL4.1_10g010500.1.01 | 812 | 90,741.61 | 6.99 | 32.04 | 86.51 | −0.118 |
SmCrRLK1L31 | SMEL4.1_12g003870.1.01 | 847 | 93,940.44 | 5.44 | 35.97 | 81.89 | −0.188 |
SmCrRLK1L32 | SMEL4.1_12g012720.1.01 | 837 | 91,980.58 | 6.39 | 35.59 | 82.75 | −0.125 |
Protein Name | Signal Peptide Number | TMhelix Number | Most Likely Location |
---|---|---|---|
SmCrRLK1L1 | 1 | 1 | Plasma Membrane |
SmCrRLK1L2 | 1 | 1 | Plasma Membrane |
SmCrRLK1L3 | 1 | 1 | Plasma Membrane |
SmCrRLK1L4 | 1 | 1 | Plasma Membrane |
SmCrRLK1L5 | 1 | 1 | Plasma Membrane |
SmCrRLK1L6 | 1 | 1 | Mitochondrial |
SmCrRLK1L7 | 1 | 1 | Cytoplasmic |
SmCrRLK1L8 | 0 | 1 | Plasma Membrane |
SmCrRLK1L9 | 0 | 1 | Plasma Membrane |
SmCrRLK1L10 | 1 | 1 | Plasma Membrane |
SmCrRLK1L11 | 1 | 1 | Plasma Membrane |
SmCrRLK1L12 | 1 | 1 | Plasma Membrane |
SmCrRLK1L13 | 1 | 1 | Plasma Membrane |
SmCrRLK1L14 | 0 | 1 | Plasma Membrane |
SmCrRLK1L15 | 1 | 1 | Plasma Membrane |
SmCrRLK1L16 | 1 | 1 | Plasma Membrane |
SmCrRLK1L17 | 1 | 1 | Plasma Membrane |
SmCrRLK1L18 | 0 | 1 | Plasma Membrane |
SmCrRLK1L19 | 1 | 1 | Plasma Membrane |
SmCrRLK1L20 | 1 | 1 | Plasma Membrane |
SmCrRLK1L21 | 1 | 1 | Plasma Membrane |
SmCrRLK1L22 | 1 | 1 | Plasma Membrane |
SmCrRLK1L23 | 1 | 1 | Plasma Membrane |
SmCrRLK1L24 | 1 | 1 | Plasma Membrane |
SmCrRLK1L25 | 1 | 1 | Plasma Membrane |
SmCrRLK1L26 | 0 | 1 | Plasma Membrane |
SmCrRLK1L27 | 1 | 1 | Plasma Membrane |
SmCrRLK1L28 | 1 | 1 | Plasma Membrane |
SmCrRLK1L29 | 1 | 1 | Plasma Membrane |
SmCrRLK1L30 | 1 | 1 | Plasma Membrane |
SmCrRLK1L31 | 1 | 1 | Plasma Membrane |
SmCrRLK1L32 | 1 | 1 | Plasma Membrane |
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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Ma, W.; Du, J.; Yu, X.; Chen, K.; Ming, Y.; Jiang, L.; Chen, T.; Ji, D. Genome-Wide Identification and Analysis of Catharanthus roseus Receptor-like Kinase 1-like Proteins in Eggplant. Plants 2023, 12, 3379. https://doi.org/10.3390/plants12193379
Ma W, Du J, Yu X, Chen K, Ming Y, Jiang L, Chen T, Ji D. Genome-Wide Identification and Analysis of Catharanthus roseus Receptor-like Kinase 1-like Proteins in Eggplant. Plants. 2023; 12(19):3379. https://doi.org/10.3390/plants12193379
Chicago/Turabian StyleMa, Wenpeng, Juan Du, Xinlong Yu, Kai Chen, Yucheng Ming, Libo Jiang, Tong Chen, and Dongchao Ji. 2023. "Genome-Wide Identification and Analysis of Catharanthus roseus Receptor-like Kinase 1-like Proteins in Eggplant" Plants 12, no. 19: 3379. https://doi.org/10.3390/plants12193379