Genotype-Dependent Jasmonic Acid Effect on Pinus sylvestris L. Growth and Induced Systemic Resistance Indicators
Abstract
:1. Introduction
2. Results
2.1. Growth Parameters
2.2. Secondary Metabolites
2.3. Antioxidative Enzymes
3. Discussion
4. Materials and Methods
4.1. Seeds
4.2. Sowing
4.3. Treatment with Jasmonic Acid
4.4. Collection of Samples
4.5. Preparation of Extracts for Secondary Metabolite and Photosynthetic Pigment Analysis
4.6. Quantification of Chlorophyll a, b and Total Carotenoids
4.7. Determination of TPC
4.8. Determination of TFC
4.9. Sample Preparation for Antioxidant Enzyme Tests
4.9.1. Total Protein (PROT)
4.9.2. Catalase (CAT)
4.9.3. Ascorbate Peroxidase (APX)
4.9.4. Guaiacol Peroxidase (POX)
4.9.5. Glutathione Reductase (GR)
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Analysis | Reaction Solutions |
---|---|
Extraction | Solution:
|
Total protein (PROT) | Biuret reagent:
|
H2O2 | 200 μL 30% H2O2 added to 10 mL of dH2O. |
Catalase (CAT) | 40 mL of 50 mM K-phosphate buffer (pH 7.0) [54]. |
Ascorbate peroxidase (APX) | Solution:
|
Guaiacol peroxidase (POX) | Solution:
|
Glutathione reductase (GR) | Solution:
|
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Beniušytė, E.; Čėsnienė, I.; Sirgedaitė-Šėžienė, V.; Vaitiekūnaitė, D. Genotype-Dependent Jasmonic Acid Effect on Pinus sylvestris L. Growth and Induced Systemic Resistance Indicators. Plants 2023, 12, 255. https://doi.org/10.3390/plants12020255
Beniušytė E, Čėsnienė I, Sirgedaitė-Šėžienė V, Vaitiekūnaitė D. Genotype-Dependent Jasmonic Acid Effect on Pinus sylvestris L. Growth and Induced Systemic Resistance Indicators. Plants. 2023; 12(2):255. https://doi.org/10.3390/plants12020255
Chicago/Turabian StyleBeniušytė, Emilija, Ieva Čėsnienė, Vaida Sirgedaitė-Šėžienė, and Dorotėja Vaitiekūnaitė. 2023. "Genotype-Dependent Jasmonic Acid Effect on Pinus sylvestris L. Growth and Induced Systemic Resistance Indicators" Plants 12, no. 2: 255. https://doi.org/10.3390/plants12020255