Genome-Wide Characterization of bHLH Family Genes and Expression Analysis in Response to Osmotic Stress in Betula platyphylla
Abstract
:1. Introduction
2. Results
2.1. Identification of bHLH Genes in Birch
2.2. Chromosomal Distribution and Collinearity Analysis of BpbHLH Genes
2.3. Phylogenetic Tree Analysis and Gene Structure Organization of BpbHLH Proteins
2.4. Conserve Motifs and Promoter Cis-Elements Analysis
2.5. Relative Expression Analysis of BpbHLH Genes under Osmotic Stress
2.6. Analysis of Relative Expression Patterns of Eight bHLH Genes Selected in Roots, Stems, and Leaves
2.7. Analysis of Relative Expression Patterns of Eight Selected BpbHLH Genes in Roots, Stems, and Leaves under PEG6000 Stress
3. Discussion
4. Materials and Methods
4.1. Identification of bHLH Family Genes in Birch
4.2. Intron and Exon Structures and Arrangement of Conserved Motifs
4.3. Chromosomal Location and Collinearity Analysis
4.4. Gene Expression Profiles of the BpbHLH Family
4.5. Plant Materials and Stress Treatment
4.6. RNA Isolation and Real-Time Polymerase Chain Reaction (qRT-PCR)
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, L.; Bi, W.; Jia, Y.; Shi, J.; Chi, Y.; Yu, M.; Wang, C. Genome-Wide Characterization of bHLH Family Genes and Expression Analysis in Response to Osmotic Stress in Betula platyphylla. Plants 2023, 12, 3687. https://doi.org/10.3390/plants12213687
Zhao L, Bi W, Jia Y, Shi J, Chi Y, Yu M, Wang C. Genome-Wide Characterization of bHLH Family Genes and Expression Analysis in Response to Osmotic Stress in Betula platyphylla. Plants. 2023; 12(21):3687. https://doi.org/10.3390/plants12213687
Chicago/Turabian StyleZhao, Leifei, Weiyi Bi, Yaqi Jia, Jingjing Shi, Yao Chi, Mingyu Yu, and Chao Wang. 2023. "Genome-Wide Characterization of bHLH Family Genes and Expression Analysis in Response to Osmotic Stress in Betula platyphylla" Plants 12, no. 21: 3687. https://doi.org/10.3390/plants12213687