A Study on the C, N, and P Contents and Stoichiometric Characteristics of Forage Leaves Based on Fertilizer-Reconstructed Soil in an Alpine Mining Area
Abstract
:1. Introduction
2. Materials and Methods
2.1. Overview of the Study Area
2.2. Sampling Design and Sample Collection
2.2.1. Sampling Design
2.2.2. Sample Collection
2.3. Nutrient Content Determination
2.4. Data Processing and Statistical Analysis
3. Results
3.1. C, N, and P Contents of Forage Leaves
3.2. Ecological Stoichiometric Characteristics of C, N, and P in Forage Leaves
3.3. Analysis of Organic Fertilizer Dosage and Forage Species Interactions
3.3.1. Three-Way ANOVA
3.3.2. Analysis of Significant Factors Affecting Fertilizer Dosage
3.3.3. Nutrient Changes with Organic Fertilizer Dosage
4. Discussion
4.1. Effects of Fertilizer Dosage on Leaf Nutrient Content and Ecological Chemometrics
4.2. Vegetation Nutrients and Organic Fertilizer Dosage
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Total Nitrogen (g/kg) | Total Phosphorus (g/kg) | Organic Matter (g/kg) | pH | |
---|---|---|---|---|
Coal gangue substrate | 2.24 | 0.51 | 62.50 | 7.32 |
Sheep manure | 11.2 | 2.89 | 158 | 7.80 |
Granular organic fertilizer | 34.5 | 4.69 | 450 | 7.72 |
Treatments | Granular Organic Fertilizer (kg/m2) | Sheep Manure | ||
---|---|---|---|---|
Sheep Manure to Coal Gangue Ratio | Sheep Manure Volume (m3/m2) | Sheep Manure Dosage (kg/m2) | ||
M0S0 | 0 | 0:10 | 0 | 0 |
M0S1 | 0 | 1.5:10 | 0.03 | 15 |
M0S2 | 0 | 3:10 | 0.06 | 30 |
M0S3 | 0 | 4.5:10 | 0.09 | 45 |
M1S0 | 1.2 | 0:10 | 0 | 0 |
M1S1 | 1.2 | 1.5:10 | 0.03 | 15 |
M1S2 | 1.2 | 3:10 | 0.06 | 30 |
M1S3 | 1.2 | 4.5:10 | 0.09 | 45 |
M2S0 | 2.4 | 0:10 | 0 | 0 |
M2S1 | 2.4 | 1.5:10 | 0.03 | 15 |
M2S2 | 2.4 | 3:10 | 0.06 | 30 |
M2S3 | 2.4 | 4.5:10 | 0.09 | 45 |
M3S0 | 3.6 | 0:10 | 0 | 0 |
M3S1 | 3.6 | 1.5:10 | 0.03 | 15 |
M3S2 | 3.6 | 3:10 | 0.06 | 30 |
M3S3 | 3.6 | 4.5:10 | 0.09 | 45 |
Items | C | N | P | C:N | C:P | N:P |
---|---|---|---|---|---|---|
V | ** | ** | ** | ** | ** | ** |
G | ns | ** | ns | ** | * | ** |
S | * | ** | ** | ns | * | * |
V × G | ns | ** | ns | ** | ns | ** |
V × S | ns | ** | ns | ** | * | ** |
G × S | ns | ** | ns | ** | ns | ** |
V × G × M | * | ** | ns | ** | * | ** |
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Ba, Y.; Li, X.; Ma, Y.; Chai, Y.; Li, C.; Ma, X.; Yang, Y. A Study on the C, N, and P Contents and Stoichiometric Characteristics of Forage Leaves Based on Fertilizer-Reconstructed Soil in an Alpine Mining Area. Plants 2023, 12, 3838. https://doi.org/10.3390/plants12223838
Ba Y, Li X, Ma Y, Chai Y, Li C, Ma X, Yang Y. A Study on the C, N, and P Contents and Stoichiometric Characteristics of Forage Leaves Based on Fertilizer-Reconstructed Soil in an Alpine Mining Area. Plants. 2023; 12(22):3838. https://doi.org/10.3390/plants12223838
Chicago/Turabian StyleBa, Yichen, Xilai Li, Yunqiao Ma, Yu Chai, Chengyi Li, Xinyue Ma, and Yongxiang Yang. 2023. "A Study on the C, N, and P Contents and Stoichiometric Characteristics of Forage Leaves Based on Fertilizer-Reconstructed Soil in an Alpine Mining Area" Plants 12, no. 22: 3838. https://doi.org/10.3390/plants12223838