Larvicidal and Adulticidal Activity of Essential Oils from Four Cuban Plants against Three Mosquito Vector Species
Abstract
:1. Introduction
2. Results
2.1. Yield and Chemical Composition of the Essential Oils
2.2. Larvicidal Activity
2.3. Adulticidal Activity
3. Discussion
4. Materials and Methods
4.1. Plant Material and Extraction of Essential Oil
4.2. Chemical Composition of the Essential Oils by GC/MS
4.3. Mosquito Larvae Culture and Mosquito Adults
4.4. Larvicidal Assay
4.5. Adulticidal Assay
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compounds | RI a | RI b | Relative Abundance (%) | |||
---|---|---|---|---|---|---|
EO-Cl | EO-Li | EO-Os | EO-Zp | |||
(3E)-Hexen-1-ol | 843 | 852 | 0.03 | 0.35 | ||
(3Z)-Hexen-1-ol | 852 | 857 | 0.94 | |||
1-Hexanol | 855 | 869 | 0.11 | |||
Tricyclene | 920 | 926 | 0.36 | |||
α-Pinene | 929 | 939 | 3.57 | 14.34 | ||
α-Fenchene | 949 | 953 | 0.89 | |||
Camphene | 949 | 953 | 1.28 | |||
Benzaldehyde | 961 | 960 | 0.04 | 1.11 | ||
Sabinene | 967 | 965 | 0.22 | 4.25 | 0.24 | |
β-Pinene | 978 | 975 | 0.7 | 1.5 | ||
1-Octen-3-ol | 980 | 978 | 0.04 | |||
(5Z)-Hepten-2-one | 985 | 986 | 0.06 | |||
Myrcene | 991 | 991 | 0.57 | 3.61 | ||
α-Phellandrene | 1005 | 1005 | 0.13 | |||
δ-Carene | 1010 | 1009 | 0.15 | |||
α-Terpinene | 1017 | 1018 | 0.19 | 0.31 | ||
Limonene | 1034 | 1031 | 7.24 | |||
1,8-Cineole | 1038 | 1033 | 2.57 | 8.62 | 0.53 | |
(Z)-β-Ocimene | 1040 | 1041 | 2.76 | |||
(E)-β-Ocimene | 1047 | 1050 | 0.3 | 3.28 | ||
γ-Terpinene | 1059 | 1056 | 0.14 | 0.98 | 0.4 | |
cis-Sabinene hydrate | 1069 | 1068 | 0.35 | |||
cis-Linalool oxide (furanoid) | 1070 | 1069 | 0.26 | |||
Terpinolene | 1082 | 1088 | 0.3 | 1.11 | ||
Linalool | 1099 | 1093 | 0.04 | 1.93 | 0.8 | 7.81 |
(2Z)-Menthen-1-ol | 1122 | 1110 | 0.07 | |||
(4E,6Z)-allo-Ocimene | 1126 | 1131 | 0.14 | |||
2,6-Dimethyl-2,4,6-octatriene | 1133 | 1149 | 7.6 | |||
trans-Pinocarveol | 1137 | 1147 | 0.05 | |||
(3Z)-Hexenyl 2-methylpropanoate | 1143 | 1145 | 0.23 | |||
trans-Verbenol | 1144 | 1148 | 0.14 | |||
Camphor | 1147 | 1150 | 0.16 | |||
Borneol | 1171 | 1165 | 1.46 | 0.31 | ||
Terpinen-4-ol | 1180 | 1180 | 0.54 | 0.98 | 1.23 | |
Decan-3-one | 1187 | 1188 | 0.68 | |||
Methyl salicylate | 1189 | 1191 | 0.12 | |||
α-Terpineol | 1197 | 1190 | 1.16 | 1.58 | ||
γ-Terpineol | 1201 | 1197 | 5.02 | |||
Nerol | 1225 | 1226 | 0.16 | |||
(3Z)-Hexenyl 2-methylbutanoate | 1232 | 1231 | 0.99 | |||
Hexyl 2-methylbutanoate | 1237 | 1236 | 0.41 | |||
Geraniol | 1252 | 1255 | 0.31 | |||
Geranial | 1268 | 1270 | 0.12 | |||
trans-Verbenyl acetate | 1278 | 1282 | 0.05 | |||
Bornyl acetate | 1285 | 1283 | 0.05 | |||
Thymol | 1294 | 1287 | 0.05 | |||
Dihydrocarvyl acetate | 1330 | 1305 | 0.29 | |||
δ-Elemene | 1331 | 1327 | 0.05 | 2.7 | 2.63 | 1.21 |
β-Terpinyl acetate | 1343 | 1359 | 0.09 | |||
Eugenol | 1352 | 1367 | 0.17 | 11.42 | 2.42 | |
Cycloisosativene | 1361 | 1362 | 0.18 | |||
Isoledene | 1367 | 1372 | 0.02 | |||
α-Copaene | 1370 | 1376 | 1.21 | 0.66 | 0.22 | |
Longicyclene | 1373 | 1374 | 0.5 | |||
β-Bourbonene | 1380 | 1388 | 0.9 | |||
β-Elemene | 1385 | 1392 | 4.33 | 0.67 | 0.51 | 5.39 |
Methyl eugenol | 1387 | 1383 | 3.44 | |||
(E)-Caryophyllene | 1422 | 1423 | 1.28 | 13.04 | 17.85 | 3.06 |
γ-Elemene | 1426 | 1425 | 1.4 | 0.88 | 2.19 | 0.35 |
trans-α-Bergamotene | 1434 | 1436 | 1.74 | |||
(Z)-β-Farnesene | 1442 | 1439 | 0.43 | |||
Isogermacrene D | 1442 | 1442 | 0.58 | 0.63 | ||
ε-Muurolene | 1446 | 1446 | 0.66 | 0.53 | ||
(E)-β-Farnesene | 1452 | 1457 | 0.24 | 1.64 | ||
α-Humulene | 1454 | 1455 | 1.56 | 1.47 | 3.33 | 0.94 |
allo-Aromadendrene | 1458 | 1461 | 0.53 | 1.52 | ||
9-epi-(E)-Caryophyllene | 1461 | 1467 | 0.81 | |||
2-epi-(E)-β-Caryophyllene | 1467 | 1469 | 0.44 | |||
β-Acoradiene | 1470 | 1472 | 0.27 | 0.94 | ||
Selina-4,11-diene | 1471 | 1476 | 0.3 | 0.27 | 0.58 | |
γ-Gurjunene | 1475 | 1479 | 6.64 | 0.46 | ||
Germacrene D | 1478 | 1480 | 1.99 | 0.56 | ||
α-Amorphene | 1482 | 1480 | 3.86 | |||
δ-Selinene | 1490 | 1485 | 3.27 | |||
trans-β-Bergamotene | 1492 | 1480 | 0.49 | |||
β-Selinene | 1493 | 1496 | 14.94 | 1.49 | ||
α-Zingiberene | 1496 | 1495 | 0.86 | |||
Bicyclogermacrene | 1498 | 1500 | 0.35 | |||
α-Muurolene | 1499 | 1497 | 1.51 | 0.09 | ||
α-Selinene | 1500 | 1501 | 7.23 | 1.42 | ||
ε-Amorphene | 1501 | 1497 | 0.61 | 0.29 | 0.43 | |
δ-Amorphene | 1505 | 1505 | 1.97 | |||
(E,E)-α-Farnesene | 1507 | 1508 | 0.7 | 1.3 | ||
β-Bisabolene | 1511 | 1510 | 0.94 | 5.37 | ||
γ-Cadinene | 1512 | 1513 | 2.07 | 0.32 | 0.1 | |
Sesquicineole | 1515 | 1516 | 1.02 | |||
δ-Cadinene | 1518 | 1518 | 0.45 | 1.23 | 0.18 | 0.63 |
trans-Cadina-1,4-diene | 1520 | 1524 | 3.66 | |||
β-Sesquiphellandrene | 1521 | 1523 | 1.7 | 0.37 | ||
Unidentified | 1525 | - | 1.86 | |||
7-epi-α-Selinene | 1528 | 1526 | 3.07 | |||
Selina-4(15),7(11)-diene | 1535 | 1534 | 4.4 | |||
α-Cadinene | 1536 | 1536 | 0.1 | |||
trans-α-Bisabolene | 1538 | 1538 | 0.41 | 0.15 | ||
α-Calacorene | 1540 | 1542 | 0.89 | |||
α-Elemol | 1548 | 1546 | 1.64 | 0.14 | 0.11 | |
cis-Sesquisabinene hydrate | 1549 | 1559 | 1.99 | |||
β-Calacorene | 1558 | 1555 | 1.09 | |||
(E)-Nerolidol | 1559 | 1559 | 0.19 | 0.20 | 0.15 | |
(Z)-Nerolidol | 1569 | 1568 | 0.79 | |||
(3Z)-Hexenyl benzoate | 1570 | 1571 | 0.75 | |||
Germacrene D-4-ol | 1575 | 1574 | 1.63 | |||
Spathulenol | 1578 | 1578 | 3.93 | 3.07 | ||
Caryophyllene oxide | 1581 | 1582 | 7.78 | 0.98 | 0.4 | |
Viridiflorol | 1594 | 1590 | 0.63 | 0.7 | 0.59 | |
Fokienol | 1591 | 1596 | 0.53 | |||
Guaiol | 1602 | 1600 | 8.27 | 0.21 | ||
Humulene epoxide II | 1607 | 1608 | 0.46 | 0.35 | ||
1,10-di-epi-Cubenol | 1612 | 1614 | 2.66 | 0.45 | 0.25 | |
Copaborneol | 1616 | 1613 | 1.23 | |||
1-epi-Cubenol | 1617 | 1617 | 0.86 | |||
epi-γ-Eudesmol | 1624 | 1622 | 1.08 | |||
Cubenol | 1630 | 1627 | 4.53 | |||
γ-Eudesmol | 1632 | 1632 | 0.1 | 0.14 | ||
11,11-Dimethyl-4,8-dimethylenebicyclo[7.2.0]undecan-3-ol | 1636 | 1645 | 2.97 | |||
Hinesol | 1640 | 1640 | 0.42 | 0.8 | ||
δ-Cadinol | 1641 | 1640 | 2.58 | 0.83 | ||
Selin-11-en-4α-ol | 1647 | 1650 | 0.3 | |||
α-Cadinol | 1658 | 1653 | 4.15 | 1.58 | 2.6 | 0.22 |
(Z)-Nerolidyl acetate | 1662 | 1665 | 0.16 | 2.12 | ||
Intermedeol | 1664 | 1666 | 0.77 | 0.18 | 0.22 | 1.09 |
3,10(14)-Guaiadien-11-ol | 1672 | 1672 | 4.75 | |||
(9Z)-Tetradecen-1-ol | 1676 | 1667 | 0.29 | |||
4(15),5,10(14)-Germacratrien-1α-ol | 1680 | 1678 | 2.8 | |||
14-Hydroxy-9-epi-(E)-β-caryophyllene | 1683 | 1682 | 2.08 | |||
α-Bisabolol | 1684 | 1684 | 15.33 | 0.11 | ||
epi-α-Bisabolol | 1688 | 1688 | 0.62 | |||
4(15),7-Eudesmadien-1β-ol | 1699 | 1690 | 5.19 | |||
7-Hydroxyeudesm-4-en-6-one | 1708 | 1703 | 2.94 | |||
(Z,Z)-Farnesol | 1712 | 1713 | 0.26 | |||
Eudesma-4,11-dien-2-ol | 1714 | 1714 | 0.7 | |||
Cedrenone | 1722 | 1722 | 0.21 | |||
Isolongifolol | 1729 | 1723 | 0.96 | |||
γ-Costol | 1733 | 1732 | 0.3 | |||
9(E)-Humulen-2,6-dione | 1739 | 1739 | 1.15 | |||
Drimenol | 1746 | 1745 | 3.11 | |||
β-Bisabolen-12-ol | 1762 | 1753 | 0.68 | |||
α(E)-Atlantone | 1768 | 1773 | 0.6 | |||
n-Tetradecanoic acid | 1769 | 1770 | 0.2 | |||
14-Oxy-α-muurolene | 1779 | 1776 | 0.87 | |||
(E)-Isovalencenol | 1783 | 1783 | 0.36 | |||
epi-Cryptomeridiol | 1791 | 1790 | 0.18 | |||
14-Hydroxy-δ-cadinene | 1803 | 1803 | 0.32 | |||
Total (%) | 98.93 | 96.94 | 96.09 | 99.05 |
Plant/Mosquito | LC50 (mg/L) (95% CI) | LC90 (mg/L) (95% CI) | H-Test Mosquitoes | H-Test Oils |
---|---|---|---|---|
EO-Cl | ||||
Aedes aegypti | 64.63 (49.52–81.31) | 243.82 (157.55–377.36) | 0.69 (ns) | 12.88 b |
Anopheles albitarsis | 63.54 (48.67–79.84) | 237.04 (149.26–376.45) | 12.90 b | |
Culex quinquefasciatus | 64.63 (49.52–81.81) | 243.82 (157.55–377.36) | 12.64 b | |
EO-Li | ||||
Aedes aegypti | 33.79 (24.99–45.71) | 182.77 (135.16–247.16) | 2.22 (ns) | 12.88 a |
Anopheles albitarsis | 38.41 (25.91–50.42) | 179.29 (124.09–259.05) | 12.90 a | |
Culex quinquefasciatus | 41.72 (29.14–53.94) | 187.37 (149.48–275.61) | 12.64 a | |
EO-Zp | ||||
Aedes aegypti | 182.08 (157.18–230.45) | 399.01 (291.80–781.55) | 0.69 (ns) | 12.88 c |
Anopheles albitarsis | 182.08 (157.18–230.45) | 399.01(291.80–781.55) | 12.90 c | |
Culex quinquefasciatus | 185.75 (160.64–235.97) | 396.33 (290.81–779.69) | 12.64 c | |
EO-Os (natural insecticide) | ||||
Aedes aegypti | 68.98 (53.92–85.94) | 243.96 (156.57–380.14) | 0.69 (ns) | 12.88 b |
Anopheles albitarsis | 68.98 (53.92–85.94) | 243.96 (156.57–380.14) | 12.90 b | |
Culex quinquefasciatus | 67.97 (53.41–84.23) | 234.23 (148.07–370.54) | 12.64 b |
Plant/Mosquito | LC50 (mg/L) (95% CI) | LC90 (mg/L) (95% CI) | H-Test Mosquitoes | H-Test Oils |
---|---|---|---|---|
EO-Cl | ||||
Aedes aegypti | 79.94 (59.56–98.80) | 158.87 (134.18–202.85) | 2.76 (ns) | 6.49 a |
Anopheles albitarsis | 69.76 (46.70–89.26) | 153.53 (127.98–200.66) | 5.40 (ns) | |
Culex quinquefasciatus | 78.63 (54.66–99.54) | 170.91 (142.57–224.10) | 5.40 (ns) | |
EO-Li | ||||
Aedes aegypti | 107.84 (88.17–128.60) b | 191.53 (163.94–240.61) | 6.49 * | 6.49 a,b |
Anopheles albitarsis | 97.35 (75.81–118.75) a,b | 188.23 (159.02–241.98) | 5.40 (ns) | |
Culex quinquefasciatus | 77.12 (49.34–100.04) a | 181.67 (149.59–245.68) | 5.40 (ns) | |
EO-Os (natural insecticide) | ||||
Aedes aegypti | 119.85 (96.47–147.02) | 226.67 (188.70–303.71) | 4.62 (ns) | 6.49 b |
Anopheles albitarsis | 92.97 (65.91–118.12) | 205.86 (169.08–282.33) | 5.40 (ns) | |
Culex quinquefasciatus | 101.59 (75.52–127.78) | 215.44 (177.13–295.58) | 5.40 (ns) |
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García-Díaz, J.; Souto, R.N.P.; Escalona-Arranz, J.C.; Ferreira, R.M.D.A.; Da Costa, T.S.; González-Fernández, R.; Heredia-Díaz, Y.; Chil-Núñez, I.; Vega, J.d.l.; Monzote, L.; et al. Larvicidal and Adulticidal Activity of Essential Oils from Four Cuban Plants against Three Mosquito Vector Species. Plants 2023, 12, 4009. https://doi.org/10.3390/plants12234009
García-Díaz J, Souto RNP, Escalona-Arranz JC, Ferreira RMDA, Da Costa TS, González-Fernández R, Heredia-Díaz Y, Chil-Núñez I, Vega Jdl, Monzote L, et al. Larvicidal and Adulticidal Activity of Essential Oils from Four Cuban Plants against Three Mosquito Vector Species. Plants. 2023; 12(23):4009. https://doi.org/10.3390/plants12234009
Chicago/Turabian StyleGarcía-Díaz, Jesús, Raimundo Nonato Picanço Souto, Julio César Escalona-Arranz, Ricardo Marcelo Dos Anjos Ferreira, Tiago Silva Da Costa, Rosalia González-Fernández, Yamile Heredia-Díaz, Idelsy Chil-Núñez, Jorge de la Vega, Lianet Monzote, and et al. 2023. "Larvicidal and Adulticidal Activity of Essential Oils from Four Cuban Plants against Three Mosquito Vector Species" Plants 12, no. 23: 4009. https://doi.org/10.3390/plants12234009