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Article

Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers

by
Tatiana E. Samatadze
1,
Olga Yu. Yurkevich
1,
Firdaus M. Khazieva
2,
Irina V. Basalaeva
2,
Olga M. Savchenko
2,
Svyatoslav A. Zoshchuk
1,
Alexander I. Morozov
2,
Alexandra V. Amosova
1 and
Olga V. Muravenko
1,*
1
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, Russia
2
All-Russian Institute of Medicinal and Aromatic Plants, 7 Green St., 117216 Moscow, Russia
*
Author to whom correspondence should be addressed.
Plants 2023, 12(23), 4056; https://doi.org/10.3390/plants12234056
Submission received: 23 October 2023 / Revised: 28 November 2023 / Accepted: 30 November 2023 / Published: 2 December 2023
(This article belongs to the Special Issue Evaluation of Genetic Diversity and Germplasm in Plants Using Molecular and DNA Markers)
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Abstract

The taxonomically challenging genus Calendula L. (Asteraceae) includes lots of medicinal species characterized by their high morphological and karyological variability. For the first time, a repeatome analysis of a valuable medicinal plant Calendula officinalis L. was carried out using high-throughput genome DNA sequencing and RepeatExplorer/TAREAN pipelines. The FISH-based visualization of the 45S rDNA, 5S rDNA, and satellite DNAs of C. officinalis was performed on the chromosomes of C. officinalis, C. stellata Cav., C. tripterocarpa Rupr., and C. arvensis L. Three satellite DNAs were demonstrated to be new molecular chromosome markers to study the karyotype structure. Karyograms of the studied species were constructed, their ploidy status was specified, and their relationships were clarified. Our results showed that the C. officinalis karyotype differed from the karyotypes of the other three species, indicating its separate position in the Calendula phylogeny. However, the presence of common repeats revealed in the genomes of all the studied species could be related to their common origin. Our findings demonstrated that C. stellata contributed its genome to allotetraploid C. tripterocarpa, and C. arvensis is an allohexaploid hybrid between C. stellata and C. tripterocarpa. At the same time, further karyotype studies of various Calendula species are required to clarify the pathways of chromosomal reorganization that occurred during speciation.
Keywords: Calendula; next-generation sequencing; repeatome; satDNAs; chromosome; fluorescence in situ hybridization Calendula; next-generation sequencing; repeatome; satDNAs; chromosome; fluorescence in situ hybridization

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MDPI and ACS Style

Samatadze, T.E.; Yurkevich, O.Y.; Khazieva, F.M.; Basalaeva, I.V.; Savchenko, O.M.; Zoshchuk, S.A.; Morozov, A.I.; Amosova, A.V.; Muravenko, O.V. Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers. Plants 2023, 12, 4056. https://doi.org/10.3390/plants12234056

AMA Style

Samatadze TE, Yurkevich OY, Khazieva FM, Basalaeva IV, Savchenko OM, Zoshchuk SA, Morozov AI, Amosova AV, Muravenko OV. Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers. Plants. 2023; 12(23):4056. https://doi.org/10.3390/plants12234056

Chicago/Turabian Style

Samatadze, Tatiana E., Olga Yu. Yurkevich, Firdaus M. Khazieva, Irina V. Basalaeva, Olga M. Savchenko, Svyatoslav A. Zoshchuk, Alexander I. Morozov, Alexandra V. Amosova, and Olga V. Muravenko. 2023. "Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers" Plants 12, no. 23: 4056. https://doi.org/10.3390/plants12234056

APA Style

Samatadze, T. E., Yurkevich, O. Y., Khazieva, F. M., Basalaeva, I. V., Savchenko, O. M., Zoshchuk, S. A., Morozov, A. I., Amosova, A. V., & Muravenko, O. V. (2023). Genome Studies in Four Species of Calendula L. (Asteraceae) Using Satellite DNAs as Chromosome Markers. Plants, 12(23), 4056. https://doi.org/10.3390/plants12234056

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