Dynamic Regulation of Grapevine’s microRNAs in Response to Mycorrhizal Symbiosis and High Temperature
Abstract
:1. Introduction
2. Results
2.1. Mycorrhizal Inoculation Results in Better Plant Response to High Temperatures
2.2. Grapevine miRNA Profiling
2.3. Heat- and AMF-Responsive miRNAs in Grapevine Leaves
2.4. Prediction of Grapevine miRNA Target Genes
2.5. GO and KEGG Functional Analyses of miRNA Target Genes
2.6. Prediction of Heat-Associated Regulatory Networks
3. Discussion
3.1. Mycorrhizal Plants Show Enhanced Response to High Temperature
3.2. Heat Stress Differently Affects miRNA Regulation in Mycorrhizal vs. Non-Mycorrhizal Grapevines
3.3. Mycorrhizal Species Influence microRNA Expression under Heat Stress
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Grapevine Exposure to High Temperature Treatment
4.3. Small RNA Library Construction and Sequencing
4.4. Bioinformatic Analysis and miRNA Identification
4.5. Prediction of Novel miRNA
4.6. Target Prediction of Conserved and Novel miRNAs
4.7. Differential Expression Analysis of miRNAs
4.8. Validation of miRNA and Target Gene Expression by RT-qPCR
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Factor | Pn | gs | E | WI | CHLI | NDVI | PRI |
---|---|---|---|---|---|---|---|
AMF inoculation | 0.015 * | 0.279 | 0.320 | 0.777 | 0.032 * | 0.485 | 0.222 |
Temperature | 0.269 | 0.274 | 0.001 * | <0.001 * | 0.133 | 0.036 * | <0.001 * |
Interaction | 0.990 | 0.713 | 0.658 | 0.699 | 0.347 | 0.715 | 0.707 |
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Campos, C.; Coito, J.L.; Cardoso, H.; Marques da Silva, J.; Pereira, H.S.; Viegas, W.; Nogales, A. Dynamic Regulation of Grapevine’s microRNAs in Response to Mycorrhizal Symbiosis and High Temperature. Plants 2023, 12, 982. https://doi.org/10.3390/plants12050982
Campos C, Coito JL, Cardoso H, Marques da Silva J, Pereira HS, Viegas W, Nogales A. Dynamic Regulation of Grapevine’s microRNAs in Response to Mycorrhizal Symbiosis and High Temperature. Plants. 2023; 12(5):982. https://doi.org/10.3390/plants12050982
Chicago/Turabian StyleCampos, Catarina, João Lucas Coito, Hélia Cardoso, Jorge Marques da Silva, Helena Sofia Pereira, Wanda Viegas, and Amaia Nogales. 2023. "Dynamic Regulation of Grapevine’s microRNAs in Response to Mycorrhizal Symbiosis and High Temperature" Plants 12, no. 5: 982. https://doi.org/10.3390/plants12050982