Fine Mapping of the Affecting Tillering and Plant Height Gene CHA-1 in Rice
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Analysis of the CHA-1 Mutant
2.2. Microscopic Observation of the CHA-1 Mutant
2.3. Fine-Mapping of CHA-1
2.4. Analysis of Candidate Genes of CHA-1
2.5. Expression and Evolutionary Analysis of CHA-1
2.6. CHA-1 Affects the SL Biosynthesis
3. Materials and Methods
3.1. Plant Materials and Phenotypic Characterization
3.2. Histological Analysis
3.3. Fine Mapping of CHA-1
3.4. Phylogenetic Tree Construction and Analysis
3.5. Lipase Activity Assay
3.6. RNA Extraction and Quantitative Real-Time PCR (qPCR)
3.7. Vector Construction and Transformation
3.8. GR24 Treatment
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Types | Number of Plants | Ratio | χ2(3:1) χ20.05 = 3.84 |
---|---|---|---|
High plant height | 2471 | 3.04:1 | 0.12 |
Low plant height | 812 |
Gene ID | Annotation |
---|---|
LOC_Os01g54810 | Class III lipase |
LOC_Os01g54850 | Cyclin-like F-box domain containing protein |
LOC_Os01g54860 | Enoyl-CoA hydratase/isomerase |
Marker | Primer Sequences (5′–3′) |
---|---|
RM8097-F | GCTGTCACTGACCGAGCGTAGG |
RM8097-R | TCGAGAGATCCAATCCAGTTTGC |
RM11668-F | AGTGTCTCTGGAGTTGGGAGTGG |
RM11668-R | CTGTTCTTCCAGATGGGCTTCC |
RM11669-F | AAACCGTTCCAGGGAGACTGACC |
RM11669-R | TCGTCTGATCCATCCATCCATCC |
DL1-F | AATGCGTGGGGTTTCATCTA |
DL1-R | TAGAGCATGGATAGACGGGG |
DL3-F | AGCTATGTGGTTAGGTCC |
DL3-R | TAGATGAGGAAGCCTAGT |
DL4-F | AGTGGCTAGTCACTTACA |
DL4-R | GGAAGCCTAGTATGAAGC |
DL5-F | ATTCCGGTGGCGTTTTCA |
DL5-R | CCACCAAAATTGTAGGGAGT |
DL6-F | CTCATTGTTGCCTATGAG |
DL6-R | GCACGTACGTAGTGAGAT |
DL7-F | ATCAGAAGCTCCTGACTCTT |
DL7-R | GCCGGAGAGGTAGTCGT |
DL8-F | TCTGAACTGAATGGTTCG |
DL8-R | TACAGTGGAGTCCTGCTA |
DL9-F | AGAGCACCCAAGAGTTAATC |
DL9-R | TGGCTGATATTGGGTATG |
DL10-F | GCCTTAGAGGAGGATCTTCT |
DL10-R | TTCCTTGCATCTCACGTAGG |
DL2-F | AAAAGCCCACTTTGCATGAG |
DL2-R | AGGTGTAACGAGAAAGCGGA |
RM11677-F | GTCTTGGAGCTGAGCACCTTGG |
RM11677-R | GGCCCTCCGTGTAATCCTATTCC |
qPCR-D53-F | CCAAGCAGTTTGAAGCGAC |
qPCR-D53-R | CCGCAAGTTTATCAAAGTCAA |
qPCR-D10-F | CGTGGCGATATCGATGGT |
qPCR-D10-R | CGACCTCCTCGAACGTCTT |
qPCR-D14-F | CGCCTTCGTCGGCCACTC |
qPCR-D14-R | TCGAACCCGCCGTGGTAGTC |
qPCR-D17-F | CTGTTCTTAGCGGGGTGTTC |
qPCR-D17-R | GGCGTCGAACTCGTAGAAAG |
qPCR-D3-F | TTAAGGTGGAGGGTGATTGC |
qPCR-D3-R | AAGATCCATCTGCCCTGTTG |
qPCR-D27-F | TCTGGGCTAAAGAATGAAAAG |
qPCR-D27-R | AGAGCTTGGGTCACAATCTCG |
Com-CHA-1-F | GAGCTCGGTACCCGGGGATCCTCTTGTAATTTTTGGGTATAAATAGACCC |
Com-CHA-1-R | ACGACGGCCAGTGCCAAGCTTTGTAAATTTTTGTGGTCCAAAGTGA |
GST-CHA-1-F | CCGCGTGGATCCCCGGAATTCATGGCGATCGACCTGGCG |
GST-CHA-1-R | GTCACGATGCGGCCGCTCGAGCTACTTTGCAGGGGGTGACG |
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Chen, T.; Xiao, W.; Huang, C.; Zhou, D.; Liu, Y.; Guo, T.; Chen, Z.; Wang, H. Fine Mapping of the Affecting Tillering and Plant Height Gene CHA-1 in Rice. Plants 2023, 12, 1507. https://doi.org/10.3390/plants12071507
Chen T, Xiao W, Huang C, Zhou D, Liu Y, Guo T, Chen Z, Wang H. Fine Mapping of the Affecting Tillering and Plant Height Gene CHA-1 in Rice. Plants. 2023; 12(7):1507. https://doi.org/10.3390/plants12071507
Chicago/Turabian StyleChen, Tengkui, Wuming Xiao, Cuihong Huang, Danhua Zhou, Yongzhu Liu, Tao Guo, Zhiqiang Chen, and Hui Wang. 2023. "Fine Mapping of the Affecting Tillering and Plant Height Gene CHA-1 in Rice" Plants 12, no. 7: 1507. https://doi.org/10.3390/plants12071507