Toxic Effects of Perilla frutescens (L.) Britt. Essential Oil and Its Main Component on Culex pipiens pallens (Diptera: Culicidae)
Round 1
Reviewer 1 Report
Quality publication that should be published. Detailed comments are attached.
Comments for author File: 
Comments.pdf
Author Response
Thank you very much for your time involved in reviewing the manuscript and your very encouraging comments on the merits. The specific response is in the attachment
Author Response File: Author Response.pdf
Reviewer 2 Report
Journal Plants (ISSN 2223-7747)
Manuscript ID plants-2253180
Type Article
Title Toxic effects of Perilla frutescens (L.) Britt. essential oil and its main component on Culex pipiens pallens (Diptera: Culicidae)
Authors Ruimin Zhang , Wenxing Zhang , Junnan Zheng , Jingwei Xu , Huan Wang , Jiajia Du , Yan Sun , Dan Zhou , Bo Shen
Section Phytochemistry
Special Issue Phytochemical Composition and Biological Activity
Abstract This study aimed to analyze the main components of Perilla frutescens essential oil (PE-EO), investigate the specific activity of PE-EO as a botanical insecticide and mosquito repellent, and explore whether its main constituents are potential candidates for further research. The larvicidal activity assay showed that LC50 of PE-EO and 2-hexanoylfuran was 45 and 25 mg/L, respectively. In the ovicidal activity assay, both 120 mg/L PE-EO and 80 mg/L 2-hexanoylfuran could achieved 98% egg mortality. Moreover, PE-EO and 2-hexanoylfuran showed repellency and oviposition deterrence effects. Notably, 10% PE-EO maintained a high rate of protection for 360 min. Therefore, perilla essential oil is an effective agent for mosquito control at several life stages and that its main component, 2-hexanoylfuran, is a potential candidate for developing novel plant biopesticides.
Concerns:
I was concerned about your GC-MS identification of main constituent of P. frutescens essential oil (PE-EO) as 2-hexanoylfuran (49%) CAS 14360-50-0.
images of 2 cpds in attached file: 2-hexanoylfuran perilla ketone
You cited article by Tian et al. (2014) that stated predominant components P. frutescens essential oils in China were 2-acetylfuran (max. 82.17%), perillaldehyde (max. 53.41%), caryophyllene (max. 38.34%), laurolene (max. 40.6%), 2-hexanoylfuran (max. 33.03%)
Verma et al. (2015) stated the main constituent of the essential oil of P. frutescens was identified as perilla ketone (48.6%) CAS 553-84-4 = 1-(Furan-3-yl)-4-methylpentan-1-one
Eldeghedy et al. (2022) stated the main constituent of the essential oil of P. frutescens was identified as L-perillaldehyde
Suggestions:
I found very little to change in the text.
Line 316 spelling: …chitin synthesis
Comments for author File: Comments.pdf
Author Response
Thank you very much for your time involved in reviewing the manuscript and your very encouraging comments on the merits.
The specific response is in the attachment
Author Response File: Author Response.pdf
