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Open AccessArticle
Relationships Within Bolbitis sinensis Species Complex Using RAD Sequencing
by
Liyun Nie
Liyun Nie
Yuhan Fang
Yuhan Fang
Zengqiang Xia
Zengqiang Xia
Xueying Wei
Xueying Wei
Zhiqiang Wu
Zhiqiang Wu
Prof. Zhiqiang Wu works as a researcher and doctoral supervisor at the Genome Analysis Laboratory of [...]
Prof. Zhiqiang Wu works as a researcher and doctoral supervisor at the Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen. He received his Ph.D. from the Institute of Botany, Chinese Academy of Sciences in 2012. He then conducted postdoctoral research at Umeå University, Sweden, Texas Tech University, Iowa State University, and Colorado State University. His main research takes plant organelles as a research object, using omics big data to explore the variation and mutation mechanism of organelle genomes, as well as the molecular mechanism of nucleocytoplasmic interaction, and exploring the transformation of organelle-based photosynthesis systems to improve crop yields.
,
Yuehong Yan
Yuehong Yan
Prof. Yuehong Yan currently works as a professor and doctoral supervisor at the Shenzhen Key for and [...]
Prof. Yuehong Yan currently works as a professor and doctoral supervisor at the Shenzhen Key Laboratory for Orchid Conservation and Utilization, National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen. Before that, he was an associate professor at the School of Life Sciences, Hunan University of Science and Technology. He obtained a bachelor's degree at the School of Life Sciences, Hunan University of Science and Technology, and a Ph.D. at the South China Botanical Garden, Chinese Academy of Sciences. His research fields are plant systematics, evolutionary biology, and systematic genomics.
Faguo Wang
Faguo Wang
Prof. Faguo Wang currently works as a professor at the South China Botanical Garden, Chinese Academy [...]
Prof. Faguo Wang currently works as a professor at the South China Botanical Garden, Chinese Academy of Sciences. He obtained a bachelor's degree from Huazhong Agricultural University and a doctoral degree from the Graduate School of the Chinese Academy of Sciences. His research fields are plant classification and flora geography, garden botany, conservation, and utilization of plant resources.
1
Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
3
School of Medical, Molecular and Forensic Sciences, Murdoch University, Murdoch, WA 6149, Australia
4
University of Chinese Academy of Sciences, Beijing 100049, China
5
Shenzhen Key Laboratory for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen 518114, China
*
Author to whom correspondence should be addressed.
Plants 2024, 13(14), 1987; https://doi.org/10.3390/plants13141987 (registering DOI)
Submission received: 29 May 2024
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Revised: 18 July 2024
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Accepted: 18 July 2024
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Published: 20 July 2024
Abstract
Species identification and phylogenetic relationship clarification are fundamental goals in species delimitation. However, these tasks pose challenges when based on morphologies, geographic distribution, and genomic data. Previously, two species of the fern genus Bolbitis, B. × multipinna and B. longiaurita were described based on morphological traits; they are phylogenetically intertwined with B. sinensis and fail to form monophyletic groups. To address the unclear phylogenetic relationships within the B. sinensis species complex, RAD sequencing was performed on 65 individuals from five populations. Our integrated analysis of phylogenetic trees, neighbor nets, and genetic structures indicate that the B. sinensis species complex should not be considered as separate species. Moreover, our findings reveal differences in the degree of genetic differentiation among the five populations, ranging from low to moderate, which might be influenced by geographical distance and gene flow. The Fst values also confirmed that genetic differentiation intensifies with increasing geographic distance. Collectively, this study clarifies the complex phylogenetic relationships within the B. sinensis species complex, elucidates the genetic diversity and differentiation across the studied populations, and offers valuable genetic insights that contribute to the broader study of evolutionary relationships and population genetics within the Bolbitis species.
Share and Cite
MDPI and ACS Style
Nie, L.; Fang, Y.; Xia, Z.; Wei, X.; Wu, Z.; Yan, Y.; Wang, F.
Relationships Within Bolbitis sinensis Species Complex Using RAD Sequencing. Plants 2024, 13, 1987.
https://doi.org/10.3390/plants13141987
AMA Style
Nie L, Fang Y, Xia Z, Wei X, Wu Z, Yan Y, Wang F.
Relationships Within Bolbitis sinensis Species Complex Using RAD Sequencing. Plants. 2024; 13(14):1987.
https://doi.org/10.3390/plants13141987
Chicago/Turabian Style
Nie, Liyun, Yuhan Fang, Zengqiang Xia, Xueying Wei, Zhiqiang Wu, Yuehong Yan, and Faguo Wang.
2024. "Relationships Within Bolbitis sinensis Species Complex Using RAD Sequencing" Plants 13, no. 14: 1987.
https://doi.org/10.3390/plants13141987
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