Morphology and Inheritance of Wavy Flower Form in Periwinkle (Catharanthus roseus (L.) G. Don)
1
Department of Horticultural Science, National Chiayi University, No. 300, Xuefu Rd., East Dist., Chiayi City 600355, Taiwan
2
School of Life Science, National Taiwan Normal University, 88 Ting-Chow Rd., Sec. 4, Taipei 116, Taiwan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2024, 13(16), 2272; https://doi.org/10.3390/plants13162272
Submission received: 27 June 2024
/
Revised: 10 August 2024
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Accepted: 13 August 2024
/
Published: 15 August 2024
(This article belongs to the Special Issue Selected Papers from the 3rd International Electronic Conference on Plant Sciences)
Abstract
:Periwinkle (Catharanthus roseus (L.) G. Don) is renowned for its diverse colors and resilience to harsh climates. Still, most commercial cultivars predominantly display flat petals. Using cultivars representing non-wavy, medium-wavy, and extreme-wavy flower forms, we examined morphological differences in both their mature leaves and floral organs. Phenotypes of self-pollinated (S1) and cross-pollinated (F1, F2) populations further underscored their morphological distinctions. Specifically, the extreme-wavy type displayed elliptical leaves, broader than the non-wavy type, with a pronounced acute apex and a notably wrinkled blade surface. The non-wavy type also bore intensely wavy petal margins and exhibited a smaller flower diameter, with a notable absence of a functional pistil, indicating female sterility. The insights gained allowed for early differentiation during the seedling period. This study suggests that the inheritance of these flower forms is regulated by an allele WAVY (Wv), which exhibits incomplete dominance. Concretely, the non-wavy form arises from a recessive homozygous expression (wvwv), the extreme-wavy from a dominant homozygous expression (WvWv), and the medium-wavy from a heterozygous expression (Wvwv). This study provides clarity on morphological descriptions and inheritance patterns of wavy flower forms, facilitating strategic breeding of diverse flower forms in periwinkle.
1. Introduction
Periwinkle (Catharanthus roseus (L.) G. Don), also known as Madagascar periwinkle or vinca, belongs to the Apocynaceae family. Initially an endemic species of Madagascar, it has since been domesticated in tropical and subtropical regions due to its prolonged flowering period, diverse flower colors, resilience to heat and drought, and overall strong adaptability [1]. Presently, periwinkle ranks among the world’s most popular summer potted plants [2]. Periwinkle is enriched in terpenoid indole alkaloids, positioning it as a potential treatment for certain mammalian heart diseases and some cancerous tumors [3].
The floral structure of periwinkle is similar to that of several species within the Apocynaceae family, comprised of five flat corolla lobes and a corolla tube. The stamens and pistil are enveloped within the corolla tube [4,5]. In 2001, a double-flowered mutant, TYV1, was first discovered in the self-pollinated lines (S1) of the cultivar ‘Pacifica Polka Dot’ in Taoyuan City, Taiwan [6], which is also the parent for breeding the doubled-flower cultivar ‘Taoyuan No.1-Rose Girl’ [7]. By 2009, double-flowered periwinkle cultivars were introduced by Sakata Seed Co., Japan. The mutation of TYV1 was not caused by the typical transformation of stamens into petaloid organs and was recognized as a single recessive allele inheritance [6]. Companies like Suntory Flower Co. (Tokyo, Japan) have released special flower forms of periwinkles such as the ‘Soiree Kawaii’ series (mini flower forms) and the ‘Soiree Flamenco’ series (wavy flower forms). Despite these advancements, no study has reported on the description or inheritance pattern of the wavy flower form in periwinkle yet. In many other flowers, wavy petals are prevalent due to their improved aesthetic value and are often a targeted trait for breeders. Understanding the inheritance pattern of the wavy flower form can lead to more precise and efficient breeding programs. Former studies have revealed that the wavy flower form of Primula polyantha is controlled by an allele displaying complete dominance [8]. Unfortunately, few studies have addressed the inheritance pattern of the wavy petal phenotype, inspiring this study to investigate the morphology and inheritance pattern of periwinkles with wavy flower form.
2. Results
2.1. Morphology of Leaves of Non-Wavy, Medium-Wavy and Extreme-Wavy Types
Morphological differences between non-wavy type (NW), medium-wavy type (MW), and extreme-wavy type (EW) can be discerned in the leaf shape. The non-wavy type is oblong, with a smooth edge, obtuse to mucronate apex, and flat surface, while the medium-wavy type has similar leaves to the non-wavy type but a slightly wavy edge, and the extreme-wavy type exhibits an ovate shape with wider leaf width and acute apex compared to the non-wavy type (Figure 1A,B). Additionally, the leaf surface of the extreme-wavy type, particularly close to the midrib, is noticeably uneven and wrinkled, which can be discerned even in young plants (Figure 1C).
The leaf lengths of the non-wavy and medium-wavy types were significantly longer than that of the extreme-wavy type, and the widths of the medium-wavy and extreme-wavy types were significantly wider than that of the non-wavy type (Table 1). For the length:width ratio, the non-wavy type had the highest ratio, followed by the medium-wavy type and extreme-wavy type, a result consistent with observed leaf appearances, indicating an obvious ovate shape.
2.2. Morphology of Flowers of Non-Wavy, Medium-Wavy, and Extreme-Wavy Types
The sepal apices of non-wavy and medium-wavy types were both narrowly acuminate, while that of the extreme-wavy type was occasionally obtuse to truncate (Figure 1D). The floral organ development of the non-wavy, medium-wavy, and extreme-wavy types did not show significant differences in terms of timing (Table 2), but there were considerable differences in petal appearance (Figure 2). During the later development period (days 6–8 of flower development), the petal arrangements of both the medium-wavy and extreme-wavy types were loose, overlapping, and uneven. Particularly, the extreme-wavy type had noticeably unsmooth petals even in the early period. Conversely, the petals of the non-wavy type only loosened up the day before blooming, exhibiting a neat and flat arrangement.
The most distinct differences among the non-wavy, medium-wavy, and extreme-wavy types were found in the waviness of the petals and the size of the flower diameter. Non-wavy types had flat petals with smooth margins, and their abaxial sides were predominantly white (Figure 3). Medium-wavy-type petals appeared wavy when viewed from the front, the margins were unsmooth and slightly jagged, and the abaxial sides were also white, but with deeper green spreading from the corolla tube towards the outer margin, and irregular spikes were observed on the margin and abaxial side. Extreme-wavy-type petals appeared wavy as well, with more pronounced and irregular jaggedness at the margin compared to the medium-wavy type, and the abaxial side exhibited deeper and more prominent green spreading than the non-wavy and medium-wavy types.
After flower wilting, the flowers of both the non-wavy and medium-wavy types fell off naturally, leaving behind the ovary and calyx on the plant. In contrast, the wilted flowers of the extreme-wavy type stuck to the plant due to the abnormal growth of the pistils (Figure 4A,B). In the cross-section of the floral organs, the stamens and pistils of the non-wavy and medium-wavy types were normal and fertile, while the stamens of the extreme-wavy type were fertile as well (Figure 4C); However, its pistils were abnormally developed (Figure 4A), consisting of two closely intertwined blades with the middle and bottom segments rolling inward (Figure 4B); several ovule-like and placenta-like organs located at the bottom segment (Figure 4D); and the end segment unfolded, presenting an irregular shape where the two structures tightly wrapped each other (Figure 4B).
The flower diameter of the non-wavy type was significantly the largest, followed by the medium-wavy type and the extreme-wavy type (Table 1). The wave height at the petal margins of the medium-wavy type was the greatest, followed by the extreme-wavy type, and the petals of non-wavy type were flat, lacking significant waves. Regarding petal weight, the medium-wavy type had the highest fresh and dry weight of petals, and there were no significant differences between the fresh and dry weight of the petals for the non-wavy and extreme-wavy types (Table 1).
2.3. Inheritance of Non-Wavy, Medium-Wavy, and Extreme-Wavy Types
Seven commercial cultivars of periwinkle were selected for this study: five non-wavy types (NW), namely, ‘Natsu Sakura’ (Summer Cherry Blossom, SS), ‘Jams ‘N Jellies Blackberry’ (JJB), ‘Mediterranean XP Rose Halo’ (MRH), ‘Tattoo Papaya’ (TP), and ‘Tattoo Blackberry’ (TB); one medium-wavy type (MW) with slightly wavy petals, ‘Furau’ (Frau, FR); and one extreme-wavy type (EW) with intensely wavy petals, ‘Purinsesu Chuchu Reddo’ (Princess Tutu Red, PR).
The petal waviness of the self-pollinated lines (S1) of non-wavy-type parents (SS, JJB, MRH, TP, and TB) did not segregate and only produced non-wavy-type offspring (Table 3), indicating that these parents were possibly homozygous. On the other hand, the petal waviness of the S1 generation of medium-wavy-type parents (FR) showed segregation. A chi-square test was performed (p = 0.136; χ2 = 3.987), and the tested group had p > 0.05, indicating no significance difference between the observed ratio and the expected ratio, accepting the ratio of 1:2:1 (Table 3). This suggested that medium-wavy-type parents (FR) was heterozygous, and the medium-wavy-type and extreme-wavy-type flower forms were likely related.
In the F1 generation produced by cross-pollination between the non-wavy type and medium-wavy type (SS × FR, JJB × FR, TP × FR, and TB × FR), segregated phenotypes of the non-wavy type and medium-wavy type were observed, but the extreme-wavy type was not present. The chi-square test was performed on all cross-pollinated combinations, and all had p > 0.05, implying that the groups satisfied the 1:1 ratio (Table 3).
In the F1 population, a few individuals were randomly selected from different combinations: SS × FR-4 (MW) from SS × FR; JJB × FR-12 (MW), JJB × FR-27 (MW), and JJB × FR-6 (NW) from JJB × FR; TP × FR-3 (MW), TP × FR-19 (MW), and TP × FR-10 (NW) from TP × FR; and TB × FR-8 (MW), TB × FR-9 (MW), TB × FR-22 (MW), TB × FR-42 (MW), TB × FR-1 (NW) and TB × FR-17 (NW) from TP × FR (Figure 5).
The non-wavy type F1 individuals were self-pollinated, and the F2 populations were all non-wavy-type. In contrast, all nine F2 populations of medium-wavy-type F1 were segregated into not only the non-wavy type and medium-wavy type, but also the extreme-wavy type. All the chi-square tests returned p > 0.05, indicating that they satisfied the 1:2:1 ratio (Table 3). This is similar to the medium-wavy-type parent (FR) self-pollination line. For instance, the F2 of TP × FR-3 consisted of 72 individuals, further classified into 23 non-wavy, 28 medium-wavy, and 21 extreme-wavy types (Figure 6). The chi-square test for this group gave a χ2 value of 3.667 and a p value of 0.16, meeting the expected 1:2:1 ratio (Table 3).
For the F1 generation from cross-pollination between the non-wavy type and extreme-wavy type (MRH × PR), all 87 plants were of the medium-wavy type. In the F1 population, two medium-wavy-type individuals, MRH × PR-2 and MRH × PR-19, were selected. MRH × PR-2 and MRH × PR-19 were self-pollinated, and the F2 generation of MRH × PR-2 segregated into 22 non-wavy-type, 34 medium-wavy-type, and 15 extreme-wavy-type individuals. The chi-square test for this group resulted in χ2 = 1.507; p = 0.471. Similarly, F2 of MRH × PR-19 segregated into 13 non-wavy-type, 49 medium-wavy-type, and 25 extreme-wavy-type individuals. The chi-square test returned χ2 = 4.701; p = 0.095. Since all tests returned p > 0.05, it was deemed that they satisfied the 1:2:1 ratio (Table 3).
3. Discussion
Commercial periwinkle cultivars often lack morphological diversity, particularly with respect to their flower forms. Most of these cultivars possess non-wavy-type petals, which can diminish their novelty. In this context, introducing medium-wavy and extreme-wavy types could increase diversity in periwinkle flower forms. This study provides insights into the morphological differences and inheritance patterns of these traits.
Non-wavy, medium-wavy, and extreme-wavy types exhibit significant differences in petal wave heights and flower diameters. In the early stages of extreme-wavy-type seedlings, characteristics like leaf and calyx features can be used for their early selection and identification. Identifying medium-wavy types among seedlings can be challenging, as their leaf edges, although somewhat wavy, are not easily distinguishable from non-wavy types during the seedling stage. However, observing the bud stage for early petal loosening, a characteristic of medium-wavy types, can assist in their early identification. With these selection strategies, breeders can accelerate the process of wavy-flower periwinkle breeding.
Very few studies have delved into the inheritance of wavy flower forms. Existing research has generally determined that the inheritance of these forms is controlled by a single dominant gene. In this study, all progeny in the S1 generation of the non-wavy-type parents displayed the non-wavy type, confirming homozygosity and the absence of genes responsible for medium-wavy or extreme-wavy types. Conversely, the self-pollinated line of the medium-wavy-type parent, FR, produced offspring exhibiting non-wavy, medium-wavy, and extreme-wavy types. Moreover, all plants in the F1 generation resulting from cross-pollination between non-wavy and extreme-wavy types were of the medium-wavy type. From the observations of this study, the medium-wavy and extreme-wavy types might be controlled by the same gene, with FR being heterozygous and PR being homozygous for this gene.
Cross-pollination of the non-wavy and medium-wavy types in the F1 generation only yielded non-wavy and medium-wavy phenotypes, adhering to a 1:1 ratio. Self-pollination of the selected medium-wavy type individuals produced a segregation ratio matching that of the FR self-pollination line, following a 1:2:1 ratio for non-wavy, medium-wavy, and extreme-wavy types. In contrast, self-pollination of the selected non-wavy type individuals produced only non-wavy-type offspring. The cross-pollinated F2 offspring of the non-wavy and extreme-wavy types exhibited the same pattern as the FR self-pollination line. These findings affirm that the medium-wavy and extreme-wavy types are controlled by an intermediate dominant allele. This study corroborates that the wavy flower form in periwinkle is governed by the WAVY allele. When the allele is homozygous dominant (WvWv), the resulting phenotype is the extreme-wavy type; when it is heterozygous (Wvwv), the phenotype is the medium-wavy type; and when it is homozygous recessive (wvwv), the phenotype is the non-wavy type.
Interestingly, the extreme-wavy type is characterized by an abnormal pistil structure, which leads to female sterility and premature flower withering. This abnormality might be due to either the linkage with the wavy petal trait or the malfunction of WAVY related to pistil development, as suggested by previous breeding studies [9,10]. Alternatively, WAVY might display pleiotropy, affecting the development of multiple organs simultaneously, and influence leaf development, leading to wider, wrinkled leaves. This trait is most pronounced in the extreme-wavy type. Out of all periwinkles bred in this study, only the extreme-wavy type exhibited phenotypes of wider leaves, unevenly wrinkled leaf surfaces, and abnormal pistils. This observation leads us to infer that gene linkage is less likely to be responsible for these traits. Instead, pleiotropy seems to be a more plausible explanation for these observed phenomena.
For the non-wavy type, the wavy flower form emerges as a dominant trait, mirroring the inheritance patterns observed in Primula polyantha [8]. The creased petal phenotype strain Q467 in Japanese morning glory (Ipomoea nil) was acquired by crossing TKS with a fe mutant strain (Q441) with the transposon-inserted FEATHERED (FE) gene, which caused severe curling and deformities in the leaves. This suggests that the dysfunction of the FE gene impacted the abaxial identity of organ cell differentiation [11]. Another investigation into Japanese morning glory revealed that the maple–willow mutant’s petals became choripetalous, and its leaf wrinkles resembled those of wavy-petal-form periwinkles, with its pistil also developing abnormally [12,13].
Prior research has indicated that the TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) family influence the development of leaves and petals. For instance, the tcp mutant petal of Arabidopsis thaliana was reported to be wavy, with mutant cells at the petal edges being elliptical, unlike the round cells of the wild type [14]. In this study, we used optical microscopes to observe a similar pattern in the flower forms of the medium-wavy and extreme-wavy types. In roses (Rosa hybrida), when the function of the TCP3 gene was knocked down, the flower diameter decreased, the petal edges became jagged, the leaves wrinkled and broadened, and the calyx took on leaf-like qualities [15]. These were similar to the effect of the extreme-wavy type in the present study. While no research has delved into the TCP transcription factor in periwinkles, a microRNA study found that periwinkles harbor three miR319 that inhibit TCP transcription [16]. Consequently, we speculate that periwinkle does possess the TCP transcription factor, and the wavy flower trait may arise from a loss of function in the TCP transcription factor, leading to a unique flower shape.
4. Materials and Methods
4.1. Plant Materials
All non-wavy-type cultivars (SS, JJB, MRH, TP, and TB) were cultivated from purchased F1 hybrid seeds, whereas the medium-wavy-type (FR) and extreme-wavy-type (PR) cultivars were clonal cultivars obtained as healthy plants from the local market. In November 2019, all parental seeds were sown in a 72-hole seedling tray using a 1:1 (v/v) mixture of peat and perlite as the growing medium. The seedlings were cultivated on a growing bench with a flood irrigation system in the National Chiayi University greenhouse in Taiwan. The mean daily light intensity at noon was 1011 μmol·m−2·s−1. The mean daytime and nighttime temperatures were 32 °C and 26 °C, respectively, in summer, and 26 °C and 18 °C, respectively, in winter. Plants were fertilized weekly with water-soluble 20N–8.7P–16.6K fertilizer (Hyponex Plant Food 20-20-20, Hyponex Co., Maryville, OH, USA) at 1 g·L−1 and watered as required. Once the seedlings developed three to four pairs of fully extended true leaves, they were transplanted to 9 cm diameter plastic pots. The medium and cultivation methods used were identical to those previously described.
4.2. Morphological Investigation of Non-Wavy, Medium-Wavy, and Extreme-Wavy Types
Medium-wavy-type and extreme-wavy-type cultivars were represented by FR and PR, respectively. For observation of the leaves’ morphology, ten mature leaves from each cultivar were measured for their length and width with a digital caliper (0.01 mm resolution). For floral organ development, ten 0.5 cm young flower buds from each cultivar were marked, with the surrounding buds removed. Bud developments were measured daily at 17:00 with a digital caliper until the flowers fully opened. For the anatomical observation of the floral organ, flowers that fully opened on the first day were selected and cut open vertically, and the calyx, stamens, and pistil were observed with a stereomicroscope.
4.3. Inheritance of Non-Wavy, Medium-Wavy, and Extreme-Wavy Types
Autogamy occasionally occurs in the periwinkle bud stage, necessitating emasculation and artificial pollination for cross-pollination. This study employed the pollination method described by a previous study [5]. Emasculation was performed two days before anthesis by removing about 1.5 cm of the corolla tube with a scalpel, and paternal pollen was collected using a 5 cm fishing string to artificially apply to maternal stigma. The parental cultivars SS, JJB, MRH, TP, TB, and FR were self-pollinated and cross-pollinated, except for the extreme-wavy-type cultivar PR, which lacked a fertile pistil and therefore could not be self-pollinated. The medium-wavy type (FR) was used as the pollen parent to cross-pollinate non-wavy types (SS, JJB, TP, and TB) to generate the F1 hybrid generation. Likewise, the extreme-wavy type (PR) was crossed with the non-wavy type (MRH).
To investigate the segregation ratio, F1 was self-pollinated to produce F2, the trait-segregated generation. This generated six parent self-pollination lines (S1), five F1, and eight F2. In January 2020, 200 seeds were sown for each S1 and F1. In March 2021, approximately 30 to 200 seeds were sown for each F2. Cultivation methods and environmental conditions were consistent with those mentioned above. Upon blooming of the plant’s second flower, individual petal phenotypes of each population were recorded. A chi-square test was employed to calculate the χ2 value of the segregation ratio of the offspring flower form segregation to compare the actual ratios with the expected ratios for significance of fit.
4.4. Statistics
The experiment adopted a complete randomized design. Statistical software Costat 6.4 (Cohort software, Monterey, CA, USA) was used for data analysis, namely, analysis of variance and calculation of least significant difference to determine whether the quantitative traits of each cultivar exhibited significant differences at p < 0.05. For the inheritance pattern test, the segregation ratio was calculated and chi-square analysis was used to calculate χ2 and the probability. When p > 0.05, the test segregation ratio was accepted.
5. Conclusions
The novel medium-wavy and extreme-wavy flower types of periwinkle (Catharanthus roseus) are regulated by the WAVY gene, which exhibits incomplete dominance. Analysis of F1, F2, and self-pollinated parental populations demonstrated that the common flat flower type (non-wavy) is regulated by the homozygous recessive genotype, while the extreme-wavy type corresponds to the homozygous dominant genotype. The medium-wavy phenotype is associated with the heterozygous genotype. This study provides a foundational framework for breeders to design crossing strategies aimed at generating a broader range of flower forms, thereby enhancing the diversity available to consumers. Additionally, for breeding wavy-flower periwinkle, breeders can predict the ratio of desired flower types in the offspring based on the genotypes of the parents. The distinct characteristics of extreme-wavy plants, such as notably wide leaves with abnormal wrinkles, an acute leaf apex, and a loose bud structure, also enable early identification and selection during the breeding process.
Author Contributions
Conceptualization, T.-H.H. and R.-S.S.; methodology, T.-H.H. and R.-S.S.; software, T.-H.H. and Y.-H.C.; validation, T.-H.H. and R.-S.S.; formal analysis, T.-H.H.; investigation, T.-H.H., Y.-H.C. and Y.-C.L.; resources, T.-H.H. and R.-S.S.; data curation, T.-H.H., Y.-H.C., Y.-C.L. and R.-S.S.; writing—original draft preparation, T.-H.H.; writing—review and editing, Y.-H.C., Y.-C.L. and R.-S.S.; visualization, T.-H.H., Y.-H.C. and Y.-C.L.; supervision, R.-S.S.; project administration, R.-S.S.; funding acquisition, R.-S.S. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Data Availability Statement
The data generated in this study are included in this published article.
Acknowledgments
The authors are grateful to S.T. Hsu and Y.H. Hsu (Department of Horticultural Science, National Chiayi University) and D.M. Yeh (Department of Horticulture and Landscape Architecture, National Taiwan University) for their consultation and advisement. We also thank our laboratory colleagues for their experimental support.
Conflicts of Interest
The authors declare no conflicts of interest.
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Figure 1.
The morphology of leaves and sepals in periwinkle with non-wavy flower form (NW), medium-wavy flower form (MW), and extreme-wavy flower form (EW). (A) Front view of leaves; (B) side view of leaves; (C) top view of juvenile plants; (D) sepals. Bar = 1 cm.
Figure 1.
The morphology of leaves and sepals in periwinkle with non-wavy flower form (NW), medium-wavy flower form (MW), and extreme-wavy flower form (EW). (A) Front view of leaves; (B) side view of leaves; (C) top view of juvenile plants; (D) sepals. Bar = 1 cm.
Figure 2.
The development of flowers of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms. The appearance from 0.5 cm flower buds to anthesis and the residuals after withering; JJB = Jams ‘N Jellies Blackberry; FR = Furau; PR = Purinsesu Chuchu Reddo.
Figure 2.
The development of flowers of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms. The appearance from 0.5 cm flower buds to anthesis and the residuals after withering; JJB = Jams ‘N Jellies Blackberry; FR = Furau; PR = Purinsesu Chuchu Reddo.
Figure 3.
Flower top, front, and bottom views of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms; NW = non-wavy; MW = medium-wavy; EW = extreme-wavy; Bar = 1 cm.
Figure 3.
Flower top, front, and bottom views of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms; NW = non-wavy; MW = medium-wavy; EW = extreme-wavy; Bar = 1 cm.
Figure 4.
Morphology and anatomy of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms. (A) Unpollinated flower organs after flower withering of non-wavy (left; arrow) and medium-wavy (right; arrow) types. (B) The wilted extreme-wavy flower stuck to the abnormally developed pistil consisting of two closely intertwined blades. (C) The non-wavy and the medium-wavy flowers had normal stamens (s) and pistils (p), while the extreme-wavy flowers had normal stamens but lacked normal pistils (arrow). (D) Several placental-like and ovule-like (arrowheads) organs at the base of the extreme-wavy flower. NW = non-wavy; MW = medium-wavy; EW = extreme-wavy. Bar = 1 cm.
Figure 4.
Morphology and anatomy of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms. (A) Unpollinated flower organs after flower withering of non-wavy (left; arrow) and medium-wavy (right; arrow) types. (B) The wilted extreme-wavy flower stuck to the abnormally developed pistil consisting of two closely intertwined blades. (C) The non-wavy and the medium-wavy flowers had normal stamens (s) and pistils (p), while the extreme-wavy flowers had normal stamens but lacked normal pistils (arrow). (D) Several placental-like and ovule-like (arrowheads) organs at the base of the extreme-wavy flower. NW = non-wavy; MW = medium-wavy; EW = extreme-wavy. Bar = 1 cm.
Figure 5.
Top view of flowers of the pollen parents, seed parents, and F1 individuals selected for F2 generation wavy flower form segregation analysis.
Figure 5.
Top view of flowers of the pollen parents, seed parents, and F1 individuals selected for F2 generation wavy flower form segregation analysis.
Figure 6.
Segregation of non-wavy, medium-wavy, and extreme-wavy flower forms in the F2 generation of F1 line TP × FR-3.
Figure 6.
Segregation of non-wavy, medium-wavy, and extreme-wavy flower forms in the F2 generation of F1 line TP × FR-3.
Table 1.
Morphological survey of leaves and flowers of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms.
Table 1.
Morphological survey of leaves and flowers of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms.
| Phenotype (Cultivar) 1 | Leaf | Flower | ||||||
|---|---|---|---|---|---|---|---|---|
| Length (cm) | Width (cm) | Length:Width Ratio 3 | Diameter (cm) | Wave Height of Petal (cm) | Fresh Weight of Corolla (g) | Dry Weight of Corolla (g) | Diameter:Fresh Weight Ratio 4 | |
| NW (JJB) | 7.39 a 2 | 3.05 b | 2.44 a | 4.77 a | 0.36 c | 0.23 b | 0.3 b | 21.6 a |
| MW (FR) | 7.46 a | 3.56 a | 2.11 b | 4.27 b | 1.21 a | 0.27 a | 0.41 a | 16.16 b |
| EW (PR) | 6.45 b | 3.74 a | 1.73 c | 3.23 c | 1.02 b | 0.21 b | 0.25 b | 17.11 b |
1 NW = non-wavy; MW = medium-wavy; EW = extreme-wavy. JJB = Jams ‘N Jellies Blackberry; FR = Furau; PR = Purinsesu Chuchu Reddo. 2 Means within each column followed by different letter(s) are significantly different at p < 0.05 according to Fisher’s LSD test. 3 Means are averaged from the ratio of each examined leaf. 4 Means are averaged from the ratio of each examined corolla.
Table 2.
The daily development and growth of flower buds from the length of 0.5 cm to flowering of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms.
Table 2.
The daily development and growth of flower buds from the length of 0.5 cm to flowering of periwinkle of the non-wavy, medium-wavy, and extreme-wavy flower forms.
| Phenotype | Day | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| (Cultivar) 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| Length of flower bud (cm) | |||||||||
| NW (JJB) | 0.52 | 0.57 | 0.80 | 0.97 | 1.25 | 1.71 | 2.16 | 3.06 | 4.38 |
| MW (FR) | 0.50 | 0.61 | 0.87 | 1.08 | 1.49 | 2.21 | 2.73 | 3.92 | 4.40 |
| EW (PR) | 0.56 | 0.60 | 0.83 | 0.97 | 1.08 | 1.54 | 1.89 | 2.45 | 3.23 |
| Relative growth (cm) | |||||||||
| NW (JJB) | - | 0.05 | 0.24 | 0.16 | 0.28 | 0.46 | 0.45 | 0.90 | 1.33 |
| MW (FR) | - | 0.11 | 0.27 | 0.21 | 0.41 | 0.72 | 0.52 | 1.18 | 0.48 |
| EW (PR) | - | 0.05 | 0.23 | 0.13 | 0.11 | 0.46 | 0.34 | 0.56 | 0.78 |
1 NW = non-wavy; MW = medium-wavy; EW = extreme-wavy. JJB = Jams ‘N Jellies Blackberry; FR = Furau; PR = Purinsesu Chuchu Reddo.
Table 3.
Segregation of non-wavy, medium-wavy, and extreme-wavy flower forms in progenies of self- and cross-pollinated periwinkle cultivars.
Table 3.
Segregation of non-wavy, medium-wavy, and extreme-wavy flower forms in progenies of self- and cross-pollinated periwinkle cultivars.
| Parents/Crosses/Generation | Total | Flower Form | Test Ratio | χ2 | p x | |||
|---|---|---|---|---|---|---|---|---|
| NW | MW | EW | ||||||
| Cultivar (flower form) | ||||||||
| FR⊗ (MW) | 77 | 25 | 30 | 22 | 1:2:1 | 3.987 | 0.136 | |
| PR⊗ (EW) | - z | - | - | - | - | - | ||
| SS⊗ (NW) | 200 | 200 | 0 | 0 | 1:0:0 | all NW | ||
| JJB⊗ (NW) | 175 | 175 | 0 | 0 | 1:0:0 | all NW | ||
| MRH⊗ (NW) | 158 | 158 | 0 | 0 | 1:0:0 | all NW | ||
| TP⊗ (NW) | 100 | 100 | 0 | 0 | 1:0:0 | all NW | ||
| TB⊗ (NW) | 136 | 136 | 0 | 0 | 1:0:0 | all NW | ||
| F1 | ||||||||
| SS (NW) × FR (MW) | 70 | 33 | 37 | 0 | 1:1:0 | 0.229 | 0.633 | |
| JJB (NW) × FR (MW) | 93 | 49 | 44 | 0 | 1:1:0 | 0.269 | 0.604 | |
| TP (NW) × FR (MW) | 45 | 28 | 17 | 0 | 1:1:0 | 2.689 | 0.101 | |
| TB (NW) × FR (MW) | 60 | 37 | 23 | 0 | 1:1:0 | 3.267 | 0.071 | |
| MRH (NW) × PR (EW) | 87 | 0 | 87 | 0 | 0:1:0 | all MW | ||
| F2 | ||||||||
| SS × FR-4 y⊗ (MW) | 101 | 23 | 50 | 28 | 1:2:1 | 0.505 | 0.777 | |
| JJB × FR-12⊗ (MW) | 15 | 4 | 9 | 2 | 1:2:1 | 1.133 | 0.567 | |
| JJB × FR-27⊗ (MW) | 26 | 7 | 16 | 3 | 1:2:1 | 2.615 | 0.270 | |
| TP × FR-3⊗ (MW) | 72 | 23 | 28 | 21 | 1:2:1 | 3.667 | 0.160 | |
| TP × FR-19⊗ (MW) | 32 | 6 | 20 | 6 | 1:2:1 | 2.000 | 0.368 | |
| TB × FR-8⊗ (MW) | 67 | 11 | 37 | 19 | 1:2:1 | 2.642 | 0.267 | |
| TB × FR-9⊗ (MW) | 51 | 13 | 22 | 16 | 1:2:1 | 1.314 | 0.518 | |
| TB × FR-22⊗ (MW) | 51 | 10 | 25 | 16 | 1:2:1 | 1.431 | 0.489 | |
| TB × FR-42⊗ (MW) | 87 | 18 | 53 | 16 | 1:2:1 | 4.241 | 0.120 | |
| MRH × PR-2⊗ (MW) | 71 | 22 | 34 | 15 | 1:2:1 | 1.507 | 0.471 | |
| MRH × PR-19⊗ (MW) | 87 | 13 | 49 | 25 | 1:2:1 | 4.701 | 0.095 | |
| JJB × FR-6⊗ (NW) | 59 | 59 | 0 | 0 | 1:0:0 | all NW | ||
| TP × FR-10⊗ (NW) | 70 | 67 | 0 | 0 | 1:0:0 | all NW | ||
| TB × FR-1⊗ (NW) | 126 | 126 | 0 | 0 | 1:0:0 | all NW | ||
| TB × FR-17⊗ (NW) | 35 | 35 | 0 | 0 | 1:0:0 | all NW | ||
z Extreme-wavy flower form phenotype is unable to self-pollinate due to abnormal pistil. y Code number of selected F1 progeny. x Chi-square analysis shows p > 0.05, indicating that the observed data conform to the expected ratio. FR = Furau; PR = Purinsesu Chuchu Reddo; SS = Natsu Sakura; JJB = Jams ‘N Jellies Blackberry; MRH = Mediterranean XP Rose Halo; TP = Tattoo Papaya; TB = Tattoo Blackberry; NW = non-wavy; MW = medium-wavy; EW = extreme-wavy.
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MDPI and ACS Style
Huang, T.-H.; Lu, Y.-C.; Chen, Y.-H.; Shen, R.-S. Morphology and Inheritance of Wavy Flower Form in Periwinkle (Catharanthus roseus (L.) G. Don). Plants 2024, 13, 2272. https://doi.org/10.3390/plants13162272
AMA Style
Huang T-H, Lu Y-C, Chen Y-H, Shen R-S. Morphology and Inheritance of Wavy Flower Form in Periwinkle (Catharanthus roseus (L.) G. Don). Plants. 2024; 13(16):2272. https://doi.org/10.3390/plants13162272
Chicago/Turabian StyleHuang, Ting-Hsuan, Yi-Chien Lu, Yu-Huan Chen, and Rong-Show Shen. 2024. "Morphology and Inheritance of Wavy Flower Form in Periwinkle (Catharanthus roseus (L.) G. Don)" Plants 13, no. 16: 2272. https://doi.org/10.3390/plants13162272
APA StyleHuang, T. -H., Lu, Y. -C., Chen, Y. -H., & Shen, R. -S. (2024). Morphology and Inheritance of Wavy Flower Form in Periwinkle (Catharanthus roseus (L.) G. Don). Plants, 13(16), 2272. https://doi.org/10.3390/plants13162272
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