Transcriptome Analysis Revealed ZmPTOX1 Is Required for Seedling Development and Stress Tolerance in Maize
Abstract
:1. Introduction
2. Results
2.1. ZmPTOX1 Mutation Produces Yellow-Green Crossbanding and Affects the Photosynthetic Efficiency in Seedlings
2.2. The Expression of ZmPTOX1 Is Regulated by the Circadian Clock
2.3. Transcriptome Analysis for OE and Segregated Etiolated and Green Sections of ko#1 Seedlings
2.4. Zebra Leaf Etiolated Sections Exhibit a Marked Defect in the Expression of Genes Involved in the Circadian Rhythm and Rhythmic Stress Response
2.5. ZmPTOX1’s Expression Level Regulates Seedling Stress Resistance
3. Discussion
3.1. ZmPTOX1 Is a Novel Zebra Leaf Phenotypic Control Gene with Pleiotropic Effects
3.2. The Zebra Leaf Phenotype of wk3735 Is Co-Regulated by Internal Signaling Pathways and External Stimuli
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RNA Extraction, qRT-PCR, and RNA Sequencing
4.3. Sequence Data Analysis
4.4. Photosynthesis and Gas Exchange Measurements
4.5. Measurement of Starch and Superoxide
4.6. Assays of Protein–Protein Interactions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample Name | Group | Rep. | Total Reads | Rate of Total Mapped Reads (%) | Rate of Uniquely Mapped Reads (%) | Num. of Expressed Genes | Rate of Expressed Genes (%) |
---|---|---|---|---|---|---|---|
OE1 | OE | 1 | 26,921,898 | 98.22 | 65.09 | 17,678 | 38.21 |
OE2 | OE | 2 | 22,588,229 | 97.67 | 82.77 | 17,518 | 37.86 |
OE3 | OE | 3 | 11,001,271 | 97.27 | 82.14 | 17,517 | 37.86 |
mu1G | muG | 1 | 24,837,626 | 97.54 | 84.64 | 18,324 | 39.6 |
mu2G | muG | 2 | 22,968,295 | 97.56 | 78.88 | 18,388 | 39.74 |
mu3G | muG | 3 | 22,426,755 | 97.69 | 84.3 | 18,375 | 39.71 |
mu1W | muW | 1 | 27,333,115 | 97.47 | 83.26 | 18,992 | 41.05 |
mu2W | muW | 2 | 25,006,640 | 97.2 | 85.83 | 19,161 | 41.41 |
mu3W | muW | 3 | 24,363,229 | 97.47 | 83.87 | 18,963 | 40.98 |
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Peng, Y.; Liang, Z.; Qing, X.; Wen, M.; Yuan, Z.; Chen, Q.; Du, X.; Gu, R.; Wang, J.; Li, L. Transcriptome Analysis Revealed ZmPTOX1 Is Required for Seedling Development and Stress Tolerance in Maize. Plants 2024, 13, 2346. https://doi.org/10.3390/plants13172346
Peng Y, Liang Z, Qing X, Wen M, Yuan Z, Chen Q, Du X, Gu R, Wang J, Li L. Transcriptome Analysis Revealed ZmPTOX1 Is Required for Seedling Development and Stress Tolerance in Maize. Plants. 2024; 13(17):2346. https://doi.org/10.3390/plants13172346
Chicago/Turabian StylePeng, Yixuan, Zhi Liang, Xindong Qing, Motong Wen, Zhipeng Yuan, Quanquan Chen, Xuemei Du, Riliang Gu, Jianhua Wang, and Li Li. 2024. "Transcriptome Analysis Revealed ZmPTOX1 Is Required for Seedling Development and Stress Tolerance in Maize" Plants 13, no. 17: 2346. https://doi.org/10.3390/plants13172346