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Open AccessArticle
Ribulose 1,5-bisphosphate carboxylase/oxygenase Is Required in Bradyrhizobium diazoefficiens for Efficient Soybean Root Colonization and Competition for Nodulation
by
Rocío S. Balda
Rocío S. Balda 1,†,
Carolina Cogo
Carolina Cogo 1,2,†,‡,
Ornella Falduti
Ornella Falduti 1,
Florencia M. Bongiorno
Florencia M. Bongiorno 3,
Damián Brignoli
Damián Brignoli 1,3,
Tamara J. Sandobal
Tamara J. Sandobal 1,3,
María Julia Althabegoiti
María Julia Althabegoiti 1 and
Aníbal R. Lodeiro
Aníbal R. Lodeiro 1,3,*
1
Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP) y Centro Científico Tecnológico (CCT)-La Plata, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata 1900, Argentina
2
Departamento de Ciencias Básicas, Facultad de Ingeniería, UNLP, La Plata 1900, Argentina
3
Cátedra de Genética, Facultad de Ciencias Agrarias y Forestales, UNLP, La Plata 1900, Argentina
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
Present address: Comisión Nacional de Energía Atómica (C.N.E.A.), San Carlos de Bariloche, Argentina.
Plants 2024, 13(17), 2362; https://doi.org/10.3390/plants13172362 (registering DOI)
Submission received: 19 June 2024
/
Revised: 30 July 2024
/
Accepted: 22 August 2024
/
Published: 24 August 2024
Abstract
The Hyphomicrobiales (Rhizobiales) order contains soil bacteria with an irregular distribution of the Calvin–Benson–Bassham cycle (CBB). Key enzymes in the CBB cycle are ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), whose large and small subunits are encoded in cbbL and cbbS, and phosphoribulokinase (PRK), encoded by cbbP. These genes are often found in cbb operons, regulated by the LysR-type regulator CbbR. In Bradyrhizobium, pertaining to this order and bearing photosynthetic and non-photosynthetic species, the number of cbbL and cbbS copies varies, for example: zero in B. manausense, one in B. diazoefficiens, two in B. japonicum, and three in Bradyrhizobium sp. BTAi. Few studies addressed the role of CBB in Bradyrhizobium spp. symbiosis with leguminous plants. To investigate the horizontal transfer of the cbb operon among Hyphomicrobiales, we compared phylogenetic trees for concatenated cbbL-cbbP-cbbR and housekeeping genes (atpD-gyrB-recA-rpoB-rpoD). The distribution was consistent, indicating no horizontal transfer of the cbb operon in Hyphomicrobiales. We constructed a ΔcbbLS mutant in B. diazoefficiens, which lost most of the coding sequence of cbbL and has a frameshift creating a stop codon at the N-terminus of cbbS. This mutant nodulated normally but had reduced competitiveness for nodulation and long-term adhesion to soybean (Glycine max (L.) Merr.) roots, indicating a CBB requirement for colonizing soybean rhizosphere.
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MDPI and ACS Style
Balda, R.S.; Cogo, C.; Falduti, O.; Bongiorno, F.M.; Brignoli, D.; Sandobal, T.J.; Althabegoiti, M.J.; Lodeiro, A.R.
Ribulose 1,5-bisphosphate carboxylase/oxygenase Is Required in Bradyrhizobium diazoefficiens for Efficient Soybean Root Colonization and Competition for Nodulation. Plants 2024, 13, 2362.
https://doi.org/10.3390/plants13172362
AMA Style
Balda RS, Cogo C, Falduti O, Bongiorno FM, Brignoli D, Sandobal TJ, Althabegoiti MJ, Lodeiro AR.
Ribulose 1,5-bisphosphate carboxylase/oxygenase Is Required in Bradyrhizobium diazoefficiens for Efficient Soybean Root Colonization and Competition for Nodulation. Plants. 2024; 13(17):2362.
https://doi.org/10.3390/plants13172362
Chicago/Turabian Style
Balda, Rocío S., Carolina Cogo, Ornella Falduti, Florencia M. Bongiorno, Damián Brignoli, Tamara J. Sandobal, María Julia Althabegoiti, and Aníbal R. Lodeiro.
2024. "Ribulose 1,5-bisphosphate carboxylase/oxygenase Is Required in Bradyrhizobium diazoefficiens for Efficient Soybean Root Colonization and Competition for Nodulation" Plants 13, no. 17: 2362.
https://doi.org/10.3390/plants13172362
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