Ectopic Expression of AetPGL from Aegilops tauschii Enhances Cadmium Tolerance and Accumulation Capacity in Arabidopsis thaliana
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Culture and Treatments
2.2. Phylogenetic and Conserved Motif Analysis of AetPGL Proteins
2.3. RNA Isolation and qRT-PCR
2.4. Construction of the AetPGL Expression Vector and Genetic Transformation of Arabidopsis thaliana
2.5. Phenotypic Analysis and Physiological Index Determination
2.6. Determination of Cadmium Content
2.7. RNA-Seq Analysis
2.8. Statistical Analysis
2.9. Primers
3. Results
3.1. AetPGL Conserved Motif and Phylogenetic Analysis
3.2. Cd-Induced AetPGL Expression
3.3. Overexpression of AetPGL Enhanced the Tolerance of Arabidopsis to Cd
3.4. Overexpression of AetPGL Enhanced the Antioxidant Capacity of Transgenic Arabidopsis
3.5. AetPGL Increased Cadmium Uptake by Regulating the Expression of Heavy Metal Transporters in Roots
3.6. AetPGL Promotes Phytohormone Synthesis in Aboveground Parts
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Appendix A
Gene Name | Forward Primer | Reverse Primer |
---|---|---|
pBI121-AetPGL | CGCGGATCCGCGATGGAGAGGGAAATGGCTG | CCCAAGCTTGGGCTAGTACTCACGATGCTGCTGCT |
AtACTIN | CTTAACCCAAAGGCCAACAGA | GCAAGGTCAAGACGGAGGAT |
qAetPGL | TGGATTGGTCTAAATGGT | ACGAATTTGTCATCAGGCAG |
qAtHMA3 | TGCTGCTCATAAGGCAAGCA | CTAGTCCAGTATCCGCGATCACT |
qAtNRAMP5 | ACAGCCACGGTTCGAATTG | CCGTGGGCATCCGACCA |
qAtYSL1 | TGCGAAAGGATGTGGCAGCCGTTCCCGA | CAACGCATAAGAACCTCTTTGA |
qAtIRT1 | TATCGCCAAATGGGCTTAAC | AAACCGATAGAGAATCGAGACG |
qAtZIP1 | ATGTGTTGTGCCTCGAGTGAT | ATCAACGGTAGACTCACGCC |
qAtAHP4 | GCTCCAAGATGATGCAAACCC | TCGTGCTGCTTCCCTTAAACT |
qAtRGL1 | AAGACCGGGTAGAGAGGCAT | CGTTTGCCATCCAAGCAACA |
qAtBES1 | CCGTTTTATGCGGTGTCTGC | CGAGGTTGGCACCATAGAGG |
qAtTCH4 | ATCACTTGGGGTGATGGTCG | TCGTCCCATGTTGTTCCAGG |
qAtTIFY7 | ATCATGTTATGCGCCGGGAA | GGGTGTGTCCCTACACCTTG |
qAtTIFY10B | AAAAACCGCAGCACAAGAGC | CTGAGCCAAGCTGGGTTAGT |
qAtJAZ3 | AGTAGCACAAACGGACTCGG | TCGTGACCCTTTCTTTGCGT |
KEGG | GeneID | GeneName | CdOE_1 | CdOE_2 | CdOE_3 | CdWT_1 | CdWT_2 | CdWT_3 | log2FC |
---|---|---|---|---|---|---|---|---|---|
Cytokinine | AT3G16360 | AHP4 | 10.0256 | 10.0559 | 16.1601 | 6.4350 | 11.3637 | 5.4429 | 0.6409 |
AT1G67710 | ARR11 | 1.4158 | 0.7991 | 1.3316 | 0.5446 | 0.3158 | 0.6660 | 1.2162 | |
Gibbenellin | AT1G66350 | RGL1 | 7.7941 | 9.9622 | 14.5231 | 7.6902 | 11.2410 | 4.7497 | 0.4469 |
Brassinosteroid | AT1G19350 | BES1 | 38.3013 | 22.0161 | 25.5946 | 15.7426 | 12.6653 | 19.8737 | 0.8314 |
AT5G57560 | TCH4 | 7.8951 | 50.0741 | 179.4677 | 11.7427 | 6.5290 | 29.7952 | 2.3044 | |
jasmonicacid | AT1G70700 | TIFY7 | 287.7421 | 346.7738 | 292.9672 | 302.0224 | 307.5035 | 253.3108 | 0.1042 |
AT1G72450 | JAZ6 | 62.5468 | 145.3204 | 116.0356 | 113.8443 | 153.5590 | 75.0006 | −0.0801 | |
AT1G74950 | TIFY10B | 71.2557 | 181.2355 | 115.7101 | 110.3789 | 127.3035 | 70.0456 | 0.2588 | |
AT3G17860 | JAZ3 | 13.8426 | 23.7418 | 23.2512 | 17.6589 | 19.2862 | 12.5151 | 0.2987 |
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Yu, J.; Hu, X.; Zhou, L.; Ye, L.; Zeng, T.; Du, X.; Gu, L.; Zhu, B.; Zhang, Y.; Wang, H. Ectopic Expression of AetPGL from Aegilops tauschii Enhances Cadmium Tolerance and Accumulation Capacity in Arabidopsis thaliana. Plants 2024, 13, 2370. https://doi.org/10.3390/plants13172370
Yu J, Hu X, Zhou L, Ye L, Zeng T, Du X, Gu L, Zhu B, Zhang Y, Wang H. Ectopic Expression of AetPGL from Aegilops tauschii Enhances Cadmium Tolerance and Accumulation Capacity in Arabidopsis thaliana. Plants. 2024; 13(17):2370. https://doi.org/10.3390/plants13172370
Chicago/Turabian StyleYu, Junxing, Xiaopan Hu, Lizhou Zhou, Lvlan Ye, Tuo Zeng, Xuye Du, Lei Gu, Bin Zhu, Yingying Zhang, and Hongcheng Wang. 2024. "Ectopic Expression of AetPGL from Aegilops tauschii Enhances Cadmium Tolerance and Accumulation Capacity in Arabidopsis thaliana" Plants 13, no. 17: 2370. https://doi.org/10.3390/plants13172370