Heterologous Expression of Sunflower HaHPT and HaTMT Genes Enhances Rice-Grain Vitamin E Content
Abstract
:1. Introduction
2. Results
2.1. HPLC Analysis of Vitamin E Isomers
2.2. Acquisition and Identification of Transgenic Plants
2.3. Relative Expression Analysis of Transgenic Plants
2.4. Changes of Main Agronomic Traits of Transgenic Plants
2.5. Vitamin E Content in Transgenic Plants
2.6. Transcriptome and Expression Analysis
2.6.1. Number of Differentially Express Genes (DEG) Statistics
2.6.2. GO Enrichment Analysis of Differentially Expressed Genes
2.6.3. KEGG Enrichment Analysis of Differentially Expressed Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Strains, Carriers and Main Reagents
4.3. Primer Design
4.4. Vector Construction
4.5. Identification of Transgenic Rice
4.6. qRT-PCR Analysis
4.7. Extraction of Vitamin E
4.8. HPLC Analysis
4.9. Transcriptome Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Song, S.; Li, H.; Lin, S.; Dong, X.; Tian, R.; Wu, Z.; Li, Q.; Li, M.; Zhang, K.; Liu, X.; et al. Heterologous Expression of Sunflower HaHPT and HaTMT Genes Enhances Rice-Grain Vitamin E Content. Plants 2024, 13, 2392. https://doi.org/10.3390/plants13172392
Song S, Li H, Lin S, Dong X, Tian R, Wu Z, Li Q, Li M, Zhang K, Liu X, et al. Heterologous Expression of Sunflower HaHPT and HaTMT Genes Enhances Rice-Grain Vitamin E Content. Plants. 2024; 13(17):2392. https://doi.org/10.3390/plants13172392
Chicago/Turabian StyleSong, Shuang, Hang Li, Shaoyan Lin, Xiaoou Dong, Ruiping Tian, Zewan Wu, Qing Li, Mingyi Li, Keying Zhang, Xi Liu, and et al. 2024. "Heterologous Expression of Sunflower HaHPT and HaTMT Genes Enhances Rice-Grain Vitamin E Content" Plants 13, no. 17: 2392. https://doi.org/10.3390/plants13172392