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Volume 13
Issue 21
10.3390/plants13213025
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Article

MsFtsH8 Enhances the Tolerance of PEG-Simulated Drought Stress by Boosting Antioxidant Capacity in Medicago sativa L.

by
Ruyue Li
1,
Xiangcui Zeng
1,
Xueqian Jiang
1,
Ruicai Long
1,
Fei He
1,
Xue Wang
1,
Lin Chen
1,
Qianwen Yu
1,
Junmei Kang
1,
Qingchuan Yang
1,
Tianhui Yang
2,3,*,
Zhongkuan Liu
4 and
Mingna Li
1,*
1
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China
3
Institute of Animal Sciences, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China
4
Institute of Agricultural Resources and Environment Research, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China
*
Authors to whom correspondence should be addressed.
Plants 2024, 13(21), 3025; https://doi.org/10.3390/plants13213025
Submission received: 27 August 2024 / Revised: 22 October 2024 / Accepted: 24 October 2024 / Published: 29 October 2024
(This article belongs to the Special Issue Stress Biology of Turfgrass—2nd Edition)
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Abstract

Drought is a major abiotic stress that limits the growth and yield of alfalfa, a vital forage legume. The plant metalloproteinase Filamentation temperature-sensitive H (FtsH) is an ATP- and Zn2+-dependent enzyme that plays a significant character in the plant’s response to environmental stress. However, its functional role in drought resistance remains largely unexplored. This study investigates the drought tolerance role of alfalfa MsFtsH8 by analyzing the growth, physiology, and gene expression of overexpressing plants under drought conditions. The results demonstrated that both MsFtsH8-overexpressing Arabidopsis and alfalfa plants exhibited superior growth condition and enhanced membrane stability. The overexpressing alfalfa plants also showed reduced MDA levels, higher proline content, lower H2O2 accumulation, an increased activity of antioxidant-related enzymes (SOD, POD, and CAT) activity, and an elevated expression of antioxidant-related genes. These results indicated that the overexpression of MsFtsH8 enhanced growth, improved osmotic regulation, reduced ROS levels, and increased antioxidative capacity, ultimately leading to greater drought tolerance in alfalfa. Our findings suggest that MsFtsH8 mitigates oxidative damage caused by drought by modulating the plant’s antioxidant system, thus improving drought tolerance in alfalfa. This study provides a molecular basis and candidate genes for enhancing drought resistance in alfalfa through genetic engineering.
Keywords: Medicago sativa L.; FtsH; drought tolerance; ROS level; antioxidant Medicago sativa L.; FtsH; drought tolerance; ROS level; antioxidant

Share and Cite

MDPI and ACS Style

Li, R.; Zeng, X.; Jiang, X.; Long, R.; He, F.; Wang, X.; Chen, L.; Yu, Q.; Kang, J.; Yang, Q.; et al. MsFtsH8 Enhances the Tolerance of PEG-Simulated Drought Stress by Boosting Antioxidant Capacity in Medicago sativa L. Plants 2024, 13, 3025. https://doi.org/10.3390/plants13213025

AMA Style

Li R, Zeng X, Jiang X, Long R, He F, Wang X, Chen L, Yu Q, Kang J, Yang Q, et al. MsFtsH8 Enhances the Tolerance of PEG-Simulated Drought Stress by Boosting Antioxidant Capacity in Medicago sativa L. Plants. 2024; 13(21):3025. https://doi.org/10.3390/plants13213025

Chicago/Turabian Style

Li, Ruyue, Xiangcui Zeng, Xueqian Jiang, Ruicai Long, Fei He, Xue Wang, Lin Chen, Qianwen Yu, Junmei Kang, Qingchuan Yang, and et al. 2024. "MsFtsH8 Enhances the Tolerance of PEG-Simulated Drought Stress by Boosting Antioxidant Capacity in Medicago sativa L." Plants 13, no. 21: 3025. https://doi.org/10.3390/plants13213025

APA Style

Li, R., Zeng, X., Jiang, X., Long, R., He, F., Wang, X., Chen, L., Yu, Q., Kang, J., Yang, Q., Yang, T., Liu, Z., & Li, M. (2024). MsFtsH8 Enhances the Tolerance of PEG-Simulated Drought Stress by Boosting Antioxidant Capacity in Medicago sativa L. Plants, 13(21), 3025. https://doi.org/10.3390/plants13213025

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