Genome-Wide Bioinformatics Analysis of SWEET Gene Family and Expression Verification of Candidate PaSWEET Genes in Potentilla anserina
Abstract
:1. Introduction
2. Results
2.1. Characterization of P. anserina SWEETs Family Genes
2.2. Analysis of Chromosomal Location of the PaSWEET Genes
2.3. Phylogenetic Analysis of Conserved Motif and Gene Structure of SWEET Genes
2.4. Analysis of Cis-Regulatory Element in the Promoter Region of PaSWEETs
2.5. Analysis of Relative Gene Expression of PaSWEETs Genes in Various Tissues
2.6. Relative Expression of PaSWEETs Genes in Swollen and Unswollen Tubers
2.7. Subcellular Location Analysis of PaSWEET Gene
2.8. Protein Interaction Network of PaSWEETs in Potentilla anserina
3. Discussion
4. Materials and Methods
4.1. Genome-Wide Identification of SWEET Genes in Potentilla anserina
4.2. Chromosomal Location and Tandem Duplication Analysis
4.3. Gene Structure, Conserved Motif, and Phylogenetic Association Analysis
4.4. Cis-Acting Element Analysis
4.5. Protein Interaction Analysis of PaSWEETs
4.6. Relative Gene Expression Analysis of PaSWEETs
4.7. Subcellular Location Analysis of PaSWEET
4.8. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Ruan, Y.L. Sucrose metabolism: Gateway to diverse carbon use and sugar signaling. Plant Biol. 2014, 65, 33–67. [Google Scholar] [CrossRef]
- Truernit, E. Plant physiology: The importance of sucrose transporters. Curr. Biol. 2001, 11, 169–171. [Google Scholar]
- Eom, J.S.; Chen, Q.; Sosso, D.; Julius, B.T.; Lin, I.W.; Qu, X.Q.; Braun, D.M.; Frommer, W.B. SWEETs, transporters for intracellular and intercellular sugar translocation. Curr. Opin. Plant Biol. 2015, 25, 53–62. [Google Scholar]
- Chen, L.Q.; Hou, B.H.; Lalonde, S.; Takanaga, H.; Hartung, M.L.; Qu, X.Q.; Guo, W.J.; Kim, J.G.; Underwood, W.; Chaudhuri, B. Sugar transporters for intercellular exchange and nutrition of pathogens. Nature 2010, 468, 527–532. [Google Scholar] [CrossRef] [PubMed]
- Patil, G.; Valliyodan, B.; Deshmukh, R.; Prince, S.; Nicander, B.; Zhao, M.; Sonah, H.; Song, L.; Lin, L.; Chaudhary, J. Soybean (Glycine max) SWEET gene family: Insights through comparative genomics, transcriptome profiling and whole genome resequence analysis. BMC Genom. 2015, 16, 520. [Google Scholar] [CrossRef] [PubMed]
- Li, W.; Ren, Z.; Sun, K.; Pei, X.; Liu, Y.; Liu, Y.; He, K.; Zhang, F.; Song, C.; Zhou, X.; et al. Evolution and stress responses of Gossypium hirsutum SWEET genes. Int. J. Mol. Sci. 2018, 19, 769. [Google Scholar] [CrossRef]
- Qin, J.; Jiang, Y.; Lu, Y.; Zhao, P.; Wu, B.; Li, H.; Wang, Y.; Xu, S.; Sun, Q.; Liu, Z. Genome-wide identification and transcriptome profiling reveal great expansion of SWEET gene family and their wide-spread responses to abiotic stress in wheat (Triticum aestivum L.). J. Integr. Agric. 2020, 19, 1704–1720. [Google Scholar] [CrossRef]
- Chong, J.; Piron, M.C.; Meyer, S.; Merdinoglu, D.; Bertsch, C.; Mestre, P. The SWEET family of sugar transporters in grapevine: VvSWEET4 is involved in the interaction with Botrytis cinerea. J. Exp. Bot. 2014, 65, 6589–6601. [Google Scholar] [CrossRef] [PubMed]
- Mizuno, H.; Kasuga, S.; Kawahigashi, H. The sorghum SWEET gene family: Stem sucrose accumulation as revealed through transcriptome profiling. Biotechnol Biofuels 2016, 9, 127. [Google Scholar] [CrossRef]
- Li, Y.; Wang, Y.; Zhang, H.; Zhang, Q.; Zhai, H.; Liu, Q.; He, S. The plasma membrane-localized sucrose transporter IbSWEET10 contributes to the resistance of sweet potato to Fusarium oxysporum. Front Plant Sci. 2017, 8, 197. [Google Scholar] [CrossRef]
- Miao, H.; Sun, P.; Liu, Q.; Miao, Y.; Liu, J.; Zhang, K.; Hu, W.; Zhang, J.; Wang, J.; Wang, Z. Genome-wide analyses of SWEET family proteins reveal involvement in fruit development and abiotic/biotic stress responses in banana. Sci. Rep. 2017, 7, 3536. [Google Scholar] [CrossRef]
- Zhou, Y.; Liu, L.; Huang, W.; Yuan, M.; Zhou, F.; Li, X.; Lin, Y. Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. Plant Biol. 2019, 13, 45–56. [Google Scholar] [CrossRef] [PubMed]
- Tao, Y.Y.; Cheung, L.S.; Li, S.; Eom, J.S.; Chen, L.Q.; Xu, Y.; Perry, K.; Frommer, W.B.; Feng, L. Structure of a eukaryotic SWEET transporter in a homotrimeric complex. Nature 2015, 527, 259–263. [Google Scholar] [CrossRef]
- Wang, J.; Yan, C.; Li, Y.; Hirata, K.; Yamamoto, M.; Yan, N.; Hu, Q. Crystal structure of a bacterial homologue of SWEET transporters. Cell Res. 2014, 24, 1486–1489. [Google Scholar] [CrossRef] [PubMed]
- Chen, L.Q.; Cheung, L.S.; Feng, L.; Tanner, W.; Frommer, W.B. Transport of sugars. Annu. Rev. Biochem. 2015, 84, 865–894. [Google Scholar] [CrossRef] [PubMed]
- Lin, I.W.; Sosso, D.; Chen, L.Q.; Gase, K.; Kim, S.G.; Kessler, D.; Klinkenberg, P.M.; Gorder, M.K.; Hou, B.H.; Qu, X.Q. Nectar secretion requires sucrose phosphate synthases and the sugar transporter SWEET9. Nature 2014, 508, 546–549. [Google Scholar] [CrossRef] [PubMed]
- Gautam, T.; Madhushree, D.; Vandana, J.; Gaurav, Z.; Vijay, G.; Sanjay, K. Emerging roles of sweet sugar transporters in plants development and abiotic stress responses. Cells 2022, 11, 1303. [Google Scholar] [CrossRef]
- Zhang, C.; Bian, Y.; Hou, S.; Li, X. Sugar transport played a more important role than sugar biosynthesis in fruit sugar accumulation during Chinese jujube domestication. Planta 2018, 248, 1187–1199. [Google Scholar] [CrossRef]
- Ren, Y.; Li, M.; Guo, S.; Sun, H.; Zhao, J.; Zhang, J.; Liu, G.; He, H.; Tian, S.; Yu, Y. Evolutionary gain of oligosaccharide hydrolysis and sugar transport enhanced carbohydrate partitioning in sweet watermelon fruits. Plant Cell. 2021, 33, 1554–1573. [Google Scholar] [CrossRef] [PubMed]
- Sosso, D.; Luo, D.; Li, Q.B.; Sasse, J.; Yang, J.; Gendrot, G.; Suzuki, M.; Koch, K.E.; McCarty, D.R.; Chourey, P.S. Seed filling in domesticated maize and rice depends on SWEET-mediated hexose transport. Nat. Genet. 2015, 47, 1489–1493. [Google Scholar] [CrossRef] [PubMed]
- Jiang, C.; Zeng, S.; Yang, J.; Wang, X. Genome-Wide Identification and Expression Profiling Analysis of SWEET Family Genes Involved in Fruit Development in Plum (Prunus salicina Lindl). Genes 2023, 14, 1679. [Google Scholar]
- Ibrahim, S.; Mohamed, G.A.; Khedr, A.; Zayed, M.F.; El-Kholy, A.A.S. Genus Hylocereus: Beneficial phytochemicals, nutritional importance, and biological relevance—A review. J. Food Biochem. 2018, 42, 12491. [Google Scholar] [CrossRef]
- Engel, M.L.; Holmes, D.R.; McCormick, S. Green sperm. Identification of male gamete promoters in Arabidopsis. Plant Physiol. 2005, 138, 2124–2133. [Google Scholar] [CrossRef]
- Wang, S.; Kengo, Y.; Runze, G.; James, W.; Yong, L.R.; Jian, F.M.; Huixia, S. The Soybean Sugar Transporter GmSWEET15 Mediates Sucrose Export from Endosperm to Early Embryo. Plant Physiol. 2019, 180, 2133–2141. [Google Scholar] [CrossRef] [PubMed]
- Chen, H.Y.; Huh, J.H.; Yu, Y.C.; Ho, L.H.; Chen, L.Q.; Tholl, D.; Frommer, W.B.; Guo, W.J. The Arabidopsis vacuolar sugar transporter SWEET2 limits carbon sequestration from roots and restricts Pythium infection. Plant J. 2015, 83, 1046–1058. [Google Scholar] [CrossRef] [PubMed]
- Hu, L.; Zhang, F.; Song, S.; Yu, X.; Ren, Y.; Zhao, X.; Liu, H.; Liu, G.; Wang, Y.; He, H. CsSWEET2, a hexose transporter from cucumber (Cucumis sativus L.), affects sugar metabolism and improves cold tolerance in Arabidopsis. Int. J. Mol. Sci. 2022, 31, 23. [Google Scholar] [CrossRef] [PubMed]
- Morikawa, T.; Ninomiya, K.; Imura, K.; Yamaguchi, T.; Akagi, Y.; Yoshikawa, M.; Hayakawa, T.; Muraoka, O. Hepatoprotective triterpenes from traditional Tibetan medicine Potentilla anserina. Phytochemistry 2014, 102, 169–180. [Google Scholar] [CrossRef]
- Liu, Z.J.; Bai, Y.; Guo, L.X.; Wang, S. Research progresses on chemical constituents of the root of Potentilla anserine L and its pharmacological activities. J. Food Saf. Qual. 2015, 6, 3569–3574. [Google Scholar]
- Li, J.Q.; Shi, J.T.; Yu, Q.L. Preliminary study on natural resource of Potentilla anserina L. Agric. Res. Arid. Areas. 2004, 2, 181–184. [Google Scholar]
- Makarov, A.A. Herbal Remedies of Yakutian Folk Medicine; Yakutsk, Russia, 1974; pp. 34–36. Available online: https://scholar.google.com/scholar_lookup?title=Herbal+Remedies+of+Yakutian+Folk+Medicine&author=A.A.+Makarov&publication_year=1974& (accessed on 1 January 2024).
- Dai, Z.; Yan, P.; He, S.; Jia, L.; Wang, Y.; Liu, Q.; Zhai, H.; Zhao, N.; Gao, S.; Zhang, H. Genome-Wide Identification and Expression Analysis of SWEET Family Genes in Sweet Potato and Its Two Diploid Relatives. Int. J. Mol. Sci. 2022, 23, 24. [Google Scholar]
- Yang, J.; Luo, D.; Yang, B.; Frommer, W.B.; Eom, J.S. SWEET11 and 15 as key players in seed filling in rice. New Phytol. 2018, 2, 604–615. [Google Scholar]
- Anjali, A.; Fatima, U.; Manu, M.S.; Ramasamy, S.; Senthil, K.M. Structure and regulation of SWEET transporters in plants: An update. Plant Physiol. Biochem. 2020, 156, 1–6. [Google Scholar] [CrossRef] [PubMed]
- Wei, X.; Liu, F.; Chen, C.; Ma, F.; Li, M. The Malus domestica sugar transporter gene family: Identifications based on genome and expression profiling related to the accumulation of fruit sugars. Front. Plant Sci. 2014, 5, 569. [Google Scholar] [CrossRef]
- Manck-Gotzenberger, J.; Requena, N. Arbuscular mycorrhiza symbiosis induces a major transcriptional reprogramming of the potato SWEET sugar transporter family. Front. Plant Sci. 2016, 7, 487. [Google Scholar] [CrossRef] [PubMed]
- Feng, C.Y.; Han, J.X.; Han, X.X.; Jiang, J. Genome-wide identification, phylogeny, and expression analysis of the SWEET gene family in tomato. Gene. 2015, 573, 261–272. [Google Scholar] [CrossRef]
- Zhang, W.; Wang, S.; Yu, F.; Tang, J.; Shan, X.; Bao, K.; Yu, L.; Wang, H.; Fei, Z.; Li, J. Genome-wide characterization and expression profiling of SWEET genes in cabbage (Brassica oleracea var. capitata L.) reveal their roles in chilling and clubroot disease responses. BMC Genom. 2019, 20, 93. [Google Scholar] [CrossRef] [PubMed]
- Li, X.Y.; Si, W.N.; Qin, Q.Q.; Wu, H.; Jiang, H.Y. Deciphering evolutionary dynamics of SWEET genes in diverse plant lineages. Sci. Rep. 2018, 8, 13440. [Google Scholar] [CrossRef]
- Nie, P.; Xu, G.; Yu, B.; Lyu, D.; Xue, X.; Qin, S. Genome-wide identification and expression profiling reveal the potential functions of the SWEET gene family during the sink organ development period in apple (Malus domestica, Borkh.). Agronomy 2022, 12, 1747. [Google Scholar] [CrossRef]
- Xie, H.H.; Wang, D.; Qin, Y.Q.; Ma, A.N.; Fu, J.X.; Qin, Y.H.; Hu, G.B.; Zhao, J.T. Genome-wide identification and expression analysis of SWEET gene family in Litchi chinensis reveal the involvement of LcSWEET2a/3b in early seed development. BMC Plant Biol. 2019, 19, 499. [Google Scholar] [CrossRef] [PubMed]
- Hu, L.P.; Zhang, F.; Song, S.H.; Tang, X.W.; Xu, H.; Liu, G.M.; Wang, Y.; He, H.J. Genome-wide identification, characterization, and expression analysis of the SWEET gene family in cucumber. J. Integr Agric. 2017, 16, 1486–1501. [Google Scholar] [CrossRef]
- Xu, G.; Guo, C.; Shan, H.; Kong, H. Divergence of duplicate genes in exon-intron structure. Proc. Natl. Acad. Sci. USA 2012, 109, 1187–1192. [Google Scholar] [CrossRef] [PubMed]
- Matsuo, T.; Yoneda, T.; Itoo, S. Identification of free cytokinins and the changes in endogenous levels during tuber development of sweet potato (Ipomoea batatas). Plant Cell Physiol. 1983, 24, 1305–1312. [Google Scholar]
- Wang, Q.M.; Zhang, L.M.; Guan, Y.A.; Wang, Z.L. Endogenous hormone concentration in developing tuberous roots of different sweet potato genotypes. Agric Sci China. 2006, 5, 919–927. [Google Scholar] [CrossRef]
- Noh, S.A.; Lee, H.S.; Huh, E.J.; Huh, G.H.; Paek, K.H. SRD1 is involved in the auxin-mediated initial thickening growth of storage root by enhancing proliferation of metaxylem and cambium cells in sweet potato (Ipomoea batatas). J. Exp. Bot. 2010, 61, 1337–1349. [Google Scholar] [CrossRef] [PubMed]
- Durand, M.; Porcheron, B.; Hennion, N.; Maurousset, L.; Lemoine, R.; Pourtau, N. Water deficit enhances C export to the roots in Arabidopsis thaliana plants with contribution of sucrose transporters in both shoot and roots. Plant Physiol. 2016, 170, 1460–1479. [Google Scholar] [CrossRef] [PubMed]
- Chen, L.Q.; Qu, X.Q.; Hou, B.H.; Sosso, D.; Osorio, S.; Fernie, A.R.; Frommer, W.B. Sucrose efflux mediated by SWEET proteins as a key step for phloem transport. Science 2012, 335, 207–211. [Google Scholar] [CrossRef]
- Geng, Y.Q.; Wu, M.J.; Zhang, C.M. Sugar transporter ZjSWEET2 mediates sugar loading in leaves of Ziziphus jujuba. Front. Plant Sci. 2020, 11, 1081. [Google Scholar] [CrossRef] [PubMed]
- Abelenda, J.; Bergonzi, S.; Oortwijn, M.; Sonnewald, S.; Du, M.; Visser, R.; Sonnewald, U.; Bachem, C. Source-sink regulation is mediated by interaction of an FT Homolog with a SWEET protein in potato. Curr. Biol. 2019, 29, 1178–1186. [Google Scholar] [CrossRef]
- Wang, P.; Peining, W.; Fangfei, N.; Xiaofenf, L.; Hongliang, Z.; Meiling, L.; Yuan, Y.; Binghua, W. Cloning and functional Assessments of Floral-Expressed SWEET Transporter Genes from Jasminum sambac. Int. J. Mol. Sci. 2019, 20, 4001. [Google Scholar] [CrossRef]
- Andres, F.; Kinoshita, A.; Kalluri, N.; Fernández, V.; Falavigna, V.S.; Cruz, T.M.; Jang, S.; Chiba, Y.; Seo, M.; Mettler, A.T. The sugar transporter SWEET10 acts downstream of FLOWERING LOCUS T during floral transition of Arabidopsis thaliana. BMC Plant Biol. 2020, 20, 1–14. [Google Scholar] [CrossRef]
- Valifard, M.; Le, H.R.; Müller, J.; Scheuring, D.; Neuhaus, H.E.; Pommerrenig, B. Vacuolar fructose transporter SWEET17 is critical for root development and drought tolerance. Plant Physiol. 2021, 187, 2716–2730. [Google Scholar] [CrossRef] [PubMed]
- Huang, D.M.; Chen, Y.; Liu, X.; Ni, D.A.; Bai, L.; Qin, Q.P. Genome-wide identification and expression analysis of the SWEETs gene family in daylily (Hemerocallis fulva) and functional analysis of HfSWEET17 in response to cold stress. BMC Plant Biol. 2022, 25, 211. [Google Scholar]
- Zhang, X.; Cao, Y.; Xin, R.; Liu, L. Genome-Wide Identification of the RsSWEET Genes Family and Functional Analysis of RsSWEET17 in Root Growth and Development in Radish. Horticulturae 2023, 6, 698. [Google Scholar] [CrossRef]
- Zhang, X.; Feng, C.; Wang, M.; Li, T.; Liu, X.; Jiang, J. Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate sugar transport and storage in tomato fruits. Hortic. Res. 2021, 81, 186. [Google Scholar] [CrossRef] [PubMed]
- Reinders, A.; Schulze, W.; Kuhn, C.; Barker, L.; Schulz, A.; Ward, J.M.; Frommer, W.B. Protein-protein interactions between sucrose transporters of different affinities colocalized in the same enucleate sieve element. Plant Cell 2002, 14, 1567–1577. [Google Scholar] [CrossRef] [PubMed]
- Wormit, A.; Oliver, T.; Ingmar, F.; Christian, L.; Joachim, T. Molecular Identification and Physiological Characterization of a Novel Monosaccharide Transporter from Arabidopsis Involved in Vacuolar Sugar Transport. Plant Cell 2006, 18, 3476–3490. [Google Scholar] [CrossRef]
- Lemonnier, P.; Cecile, G.; Florian, V.; Jeremy, V. Expression of Arabidopsis sugar transport protein STP13 differentially affects glucose transport activity and basal resistance to Botrytis cinerea. Plant Mol. Biol. 2014, 85, 473–484. [Google Scholar] [CrossRef]
- Badet, T.; Léger, O.; Barascud, M.; Voisin, D.; Sadon, P.; Vincent, R.; Le Ru, A.; Balagué, C.; Roby, D.; Raffaele, S. Expression polymorphism at the ARPC4 locus links the actin cytoskeleton with quantitative disease resistance to Sclerotinia sclerotiorum in Arabidopsis thaliana. N. Phytol. 2019, 222, 480–496. [Google Scholar]
- Slawinski, L.; Israel, A.; Artault, C.; Thibault, F.; Atanassova, R.; Laloi, M.; Dédaldé, C. Responsiveness of Early Response to Dehydration Six-Like Transporter Genes to Water Deficit in Arabidopsis thaliana Leaves. Front. Plant Sci. 2021, 12, 708876. [Google Scholar] [CrossRef]
- Finn, R.D.; Clements, J.; Eddy, S.R. HMMER web server: Interactive sequence similarity searching. Nucleic Acids Res. 2011, 39, 29–37. [Google Scholar] [CrossRef]
- Gasteiger, E.; Gattiker, A.; Hoogland, C.; Ivanyi, I.; Appel, R.D.; Bairoch, A. ExPASy: The proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 2003, 31, 3784–3788. [Google Scholar] [CrossRef]
- Howe, K.L.; Achuthan, P.; Allen, J.; Alvarez, J.J.; Amode, M.R.; Armean, I.M.; Azov, A.G.; Bennett, R.; Bhai, J. Ensembl 2021. Nucleic Acids Res. 2021, 49, 884–891. [Google Scholar] [CrossRef]
- Chou, K.C.; Shen, H.B. Cell-PLoc: A package of Web servers for predicting subcellular localization of proteins in various organisms. Nature Prot. 2008, 3, 153–162. [Google Scholar] [CrossRef] [PubMed]
- Chen, C.; Wu, Y.; Li, L.; Wang, X.; Zeng, Z.; Xu, J.; Liu, Y.; Feng, J.; Chen, H.; He, Y.; et al. TBtools-II: A “One for All, All for One” bioinformatics platform for biological big-data mining. Mol. Plant 2023, 16, 1733–1742. [Google Scholar] [CrossRef] [PubMed]
- Kumar, S.; Stecher, G.; Li, M.; Knyaz, C.; Tamura, K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol. 2018, 35, 1547–1549. [Google Scholar] [CrossRef] [PubMed]
- Lescot, M.; Dehais, P.; Thijs, G.; Marchal, K.; Moreau, Y.; Van de Peer, Y.; Rouze, P.; Rombauts, S. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 2002, 30, 325–327. [Google Scholar] [CrossRef]
- Kohl, M.; Wiese, S.; Warscheid, B. Cytoscape: Software for visualization and analysis of biological networks. Methods Mol. Biol. 2011, 696, 291–303. [Google Scholar]
- Singh, V.K.; Mangalam, A.K.; Dwivedi, S.; Naik, S. Primer premier: Program for design of degenerate primers from a protein sequence. BioTechniques 1998, 24, 318–319. [Google Scholar] [CrossRef]
Gene Name | Gene Locus | Start | End | Chr | CDS (bp) | PI | MW (KDa) | A.A | S.C. Location |
---|---|---|---|---|---|---|---|---|---|
PaSWEET1 | Poanv1_3G01215.1 | 16244178 | 16245727 | Chr3 | 756 | 9.62 | 27.46 | 251 | Cell membrane |
PaSWEET2 | Poanv1_3G02345.1 | 26445095 | 26446626 | Chr3 | 948 | 9.38 | 35.06 | 315 | Cell membrane |
PaSWEET3 | Poanv1_3G02537.1 | 27842414 | 27843899 | Chr3 | 882 | 5.82 | 32.7 | 293 | Cell membrane |
PaSWEET4 | Poanv1_4G01502.1 | 9722164 | 9723717 | Chr4 | 960 | 9.23 | 35.47 | 319 | Cell membrane |
PaSWEET5 | Poanv1_4G02299.1 | 16809415 | 16810970 | Chr4 | 753 | 9.45 | 27.33 | 250 | Cell membrane |
PaSWEET6 | Poanv1_4G02307.1 | 16970550 | 16972095 | Chr4 | 753 | 9.37 | 27.32 | 250 | Cell membrane |
PaSWEET7 | Poanv1_5G00681.1 | 4865439 | 4866991 | Chr5 | 705 | 8.95 | 26.1 | 234 | Cell membrane |
PaSWEET8 | Poanv1_5G01420.1 | 11911444 | 11913660 | Chr5 | 708 | 8.91 | 26.23 | 235 | Cell membrane |
PaSWEET9 | Poanv1_6G00874.1 | 6027126 | 6028681 | Chr6 | 705 | 8.95 | 26.18 | 234 | Cell membrane |
PaSWEET10 | Poanv1_6G01829.1 | 14477140 | 14479272 | Chr6 | 708 | 9.08 | 26.24 | 235 | Cell membrane |
PaSWEET11 | Poanv1_6G01858.1 | 14918865 | 14920993 | Chr6 | 708 | 9.08 | 26.24 | 235 | Cell membrane |
PaSWEET12 | Poanv1_7G01742.1 | 22928052 | 22929674 | Chr7 | 702 | 8.42 | 25.94 | 233 | Cell membrane |
PaSWEET13 | Poanv1_7G02056.1 | 25735871 | 25737656 | Chr7 | 915 | 6.52 | 34.02 | 304 | Cell membrane |
PaSWEET14 | Poanv1_8G01210.1 | 13751438 | 13753172 | Chr8 | 918 | 5.41 | 33.93 | 305 | Cell membrane |
PaSWEET15 | Poanv1_9G02031.1 | 23847352 | 23849568 | Chr9 | 711 | 9.28 | 26.46 | 236 | Cell membrane |
PaSWEET16 | Poanv1_9G02352.1 | 26435793 | 26437671 | Chr9 | 930 | 7.03 | 34.59 | 309 | Cell membrane |
PaSWEET17 | Poanv1_9G02531.1 | 27818865 | 27820769 | Chr9 | 762 | 9.38 | 28.12 | 253 | Cell membrane |
PaSWEET18 | Poanv1_10G01926.1 | 21562089 | 21563611 | Chr10 | 708 | 9.3 | 26.49 | 235 | Cell membrane |
PaSWEET19 | Poanv1_10G02196.1 | 23424599 | 23426888 | Chr10 | 909 | 6.93 | 34.13 | 302 | Cell membrane |
PaSWEET20 | Poanv1_10G02432.1 | 25180176 | 25182110 | Chr10 | 762 | 9.57 | 28.03 | 253 | Cell membrane |
PaSWEET21 | Poanv1_12G04149.1 | 41778738 | 41779835 | Chr12 | 705 | 9.43 | 26.68 | 234 | Cell membrane |
PaSWEET22 | Poanv1_13G00740.1 | 11203434 | 11204844 | Chr13 | 708 | 8.59 | 26.02 | 235 | Cell membrane |
PaSWEET23 | Poanv1_13G02471.1 | 26252771 | 26253791 | Chr13 | 693 | 8.99 | 26 | 230 | Cell membrane |
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Iqbal, J.; Zhang, W.; Fan, Y.; Dong, J.; Xie, Y.; Li, R.; Yang, T.; Zhang, J.; Che, D. Genome-Wide Bioinformatics Analysis of SWEET Gene Family and Expression Verification of Candidate PaSWEET Genes in Potentilla anserina. Plants 2024, 13, 406. https://doi.org/10.3390/plants13030406
Iqbal J, Zhang W, Fan Y, Dong J, Xie Y, Li R, Yang T, Zhang J, Che D. Genome-Wide Bioinformatics Analysis of SWEET Gene Family and Expression Verification of Candidate PaSWEET Genes in Potentilla anserina. Plants. 2024; 13(3):406. https://doi.org/10.3390/plants13030406
Chicago/Turabian StyleIqbal, Javed, Wuhua Zhang, Yingdong Fan, Jie Dong, Yangyang Xie, Ronghui Li, Tao Yang, Jinzhu Zhang, and Daidi Che. 2024. "Genome-Wide Bioinformatics Analysis of SWEET Gene Family and Expression Verification of Candidate PaSWEET Genes in Potentilla anserina" Plants 13, no. 3: 406. https://doi.org/10.3390/plants13030406