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Plants
Volume 14
Issue 5
10.3390/plants14050761
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group)

by
Xiaobing Zhao
1,
Yiting Zhuang
2,
Wangyang Xie
2,
Yixin Yang
2,
Jingyu Pu
2,
Zhengyang Fan
1,
Yukun Chen
1,
Yuling Lin
1,* and
Zhongxiong Lai
1,*
1
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Authors to whom correspondence should be addressed.
Plants 2025, 14(5), 761; https://doi.org/10.3390/plants14050761 (registering DOI)
Submission received: 15 December 2024 / Revised: 19 February 2025 / Accepted: 25 February 2025 / Published: 1 March 2025
(This article belongs to the Special Issue Advances in Plant Auxin Biology)
Versions Notes

Abstract

The regulatory mechanisms underlying embryogenic callus (EC) formation in polyploid bananas remain unexplored, posing challenges for genetic transformation and biotechnological applications. Here, we conducted transcriptome sequencing on cultured explants, non-embryogenic callus, EC, and browning callus in the ABB cultivar ‘MJ’ (Musa spp. cv. Bengal). Our analysis of differentially expressed genes (DEGs) revealed significant enrichment in plant hormones, MAPK, and zeatin biosynthesis pathways. Notably, most genes in the MJ variety exhibited balanced expression of the A and B alleles, but A-specific allele expression was dominant in the key signaling pathways, whereas B-specific allele expression was very rare during EC induction. In the auxin signaling pathway, six A-specific MJARF genes were markedly downregulated, underscoring their critical roles in the negative regulation of callus formation. Additionally, six A-specific MJEIN3 alleles were found to play negative regulatory roles in ethylene signaling during EC development. We also identified phenylpropanoids responsible for enzymatic browning. Furthermore, the expression patterns of transcription factors in bananas exhibited specific expression modes, highlighting the unique mechanisms of callus formation. This study enhanced our understanding of the regulatory roles of these alleles in EC induction and offers new insights into the utilization of alleles to improve the efficiency of somatic embryogenesis in bananas.
Keywords: embryogenic callus; specific allele; transcriptional regulation; browning embryogenic callus; specific allele; transcriptional regulation; browning

Share and Cite

MDPI and ACS Style

Zhao, X.; Zhuang, Y.; Xie, W.; Yang, Y.; Pu, J.; Fan, Z.; Chen, Y.; Lin, Y.; Lai, Z. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group). Plants 2025, 14, 761. https://doi.org/10.3390/plants14050761

AMA Style

Zhao X, Zhuang Y, Xie W, Yang Y, Pu J, Fan Z, Chen Y, Lin Y, Lai Z. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group). Plants. 2025; 14(5):761. https://doi.org/10.3390/plants14050761

Chicago/Turabian Style

Zhao, Xiaobing, Yiting Zhuang, Wangyang Xie, Yixin Yang, Jingyu Pu, Zhengyang Fan, Yukun Chen, Yuling Lin, and Zhongxiong Lai. 2025. "Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group)" Plants 14, no. 5: 761. https://doi.org/10.3390/plants14050761

APA Style

Zhao, X., Zhuang, Y., Xie, W., Yang, Y., Pu, J., Fan, Z., Chen, Y., Lin, Y., & Lai, Z. (2025). Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group). Plants, 14(5), 761. https://doi.org/10.3390/plants14050761

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