Plants

15 pages, 2514 KB

Open AccessArticle

Seasonal Shifts in Water Utilization Strategies of Typical Desert Plants in a Desert Oasis Revealed by Hydrogen and Oxygen Stable Isotopes and Leaf δ¹³C

by Yang Wang, Wenze Li, Wei Cai, Nan Bai, Jiaqi Wang and Yu Hong

Plants 2026, 15(2), 340; https://doi.org/10.3390/plants15020340 - 22 Jan 2026

Cited by 1 | Viewed by 387

Abstract

Understanding seasonal water acquisition strategies of desert plants is critical for predicting vegetation resilience under increasing hydrological stress in arid inland river basins. In hyper-arid oases, strong evaporative demand and declining groundwater levels impose tightly coupled constraints on plant water uptake across soil–plant–atmosphere [...] Read more.

Understanding seasonal water acquisition strategies of desert plants is critical for predicting vegetation resilience under increasing hydrological stress in arid inland river basins. In hyper-arid oases, strong evaporative demand and declining groundwater levels impose tightly coupled constraints on plant water uptake across soil–plant–atmosphere continua. In this study, we combined hydrogen and oxygen stable isotopes, Bayesian mixing models, soil moisture measurements and groundwater monitoring, and leaf δ¹³C analysis to quantify monthly water-source contributions and long-term water-use efficiency of three dominant species (Reaumuria soongarica, Tamarix ramosissima, and Populus euphratica) in the Ejina Oasis. Clear ecohydrological niche differentiation was evident among the three species. R. soongarica exhibited moderate temporal flexibility by integrating shallow and deep soil water with episodic groundwater use, whereas T. ramosissima adopted a vertically integrated and hydraulically plastic strategy combining precipitation, multi-depth soil water, and groundwater. In contrast, P. euphratica followed a conservative strategy, relying predominantly on deep soil water with only minor and transient inputs from precipitation and groundwater. Across species and seasons, deep vadose-zone soil water (120–200 cm) consistently acted as the most stable and influential reservoir, buffering seasonal drought and sustaining transpiration. T. ramosissima maintained the highest intrinsic water-use efficiency, and P. euphratica exhibited consistently lower efficiency associated with sustained access to stable deep soil water. These contrasting strategies reveal multiple pathways of hydraulic stability and plasticity that underpin vegetation persistence under progressive groundwater depletion. By linking water-source partitioning with physiological regulation, this study provides a mechanistic basis for understanding plant water-use strategies and informs ecological water management and species-specific restoration in hyper-arid inland oases. Full article

(This article belongs to the Section Plant–Soil Interactions)

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25 pages, 11228 KB

Open AccessArticle

Optimal Branch Bending Angle for Korla Fragrant Pear: A Multi-Trait Physiological Trade-Off Framework

by Ablah Niyaz, Mansur Nasir, Shikui Zhang, Shaopeng Wang, Cuihui Min, Guoquan Fan, Dilraba Muhtar, Xianbiao Ma, Mirigul Tunyaz, Lihong Yao, Ruizhe Wang, Tianming He, Juan Song and Mayira Eziz

Plants 2026, 15(2), 339; https://doi.org/10.3390/plants15020339 - 22 Jan 2026

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Abstract

The optimal branch bending angle for Pyrus sinkiangensis Yü (Korla fragrant pear) remains undefined. In this study, the optimal angle was determined by integrating the phenological, nutritional, hormonal, and fruit-quality responses across a 15-day bloom window. Four branch angles (40°, 60°, 80°, and [...] Read more.

The optimal branch bending angle for Pyrus sinkiangensis Yü (Korla fragrant pear) remains undefined. In this study, the optimal angle was determined by integrating the phenological, nutritional, hormonal, and fruit-quality responses across a 15-day bloom window. Four branch angles (40°, 60°, 80°, and 100°) were applied to 8-year-old trees in spring 2022, and flowering dynamics, bud carbon/nitrogen status, leaf morphology/mineral content, fruiting-shoot architecture, endogenous hormones, and fruit quality were comprehensively evaluated. The 80° angle maximized the fruit set (11.77%) and bud soluble sugar content (8.84 mg/g DW), significantly outperforming the other angles (p < 0.05). The flowering rate peaked at 100° (7.89%) but was statistically comparable to that at 60° and 80° (p > 0.05); calyx removal was greatest at 60° (73.33%), with no significant difference from that at 80° (71%, p > 0.05). These reproductive benefits aligned with enhanced leaf source capacity—80° pulling resulted in the greatest leaf area (59.51 cm²), the greatest amount of chlorophyll (3.11 mg/g DW), and elevated N/Mg/Cu concentrations. Branch architecture was optimized at 80°, with the percentage of medium fruiting spurs reaching 41.1% and the xylem:phloem dry-weight ratio peaking at 1.78, indicating the development of efficient assimilate transport pathways. Hormonally, 80° triggered a distinct cascade: a transient GA₄/GA₇ surge (50.6 and 1.34 ng/g DW) on 28 April, followed by sustained IAA elevation (2.05 ng/g DW) and zeatin stabilization (0.27–0.29 ng/g DW) during ovary development. Consequently, the fruit quality was comprehensively improved at 80°—the single-fruit weight (110.7 g), soluble sugar content (10.08 mg/g DW), and sugar/acid ratio (17.08) were greatest, whereas the stone-cell content was lowest (0.49 mg/g DW). Principal component analysis of 57 traits confirmed 80° as the system-wide optimum (D = 0.718). These results demonstrate that an 80° bending angle synchronizes carbohydrate supply, hormone signaling, and fruit quality in Korla fragrant pear, providing a low-cost, nonchemical benchmark for precision canopy management in high-density orchards. An 80° branch-bending angle optimizes carbon-hormone synergy via a transient GA₄/GA₇ surge and sustained IAA-zeatin signaling, maximizing fruit set and quality in high-density Korla fragrant pear orchards. Full article

(This article belongs to the Special Issue Growth, Development, and Stress Response of Horticulture Plants/Crops—2nd Edition)

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16 pages, 2058 KB

Open AccessArticle

Overexpression of BnaMATE43b Improves Resistance to Aluminum Toxicity and Identification of Its Upstream Transcription Factors in Rapeseed (Brassica napus L.)

by Xiaojun Xiao, Huiwen Zhou, Paolan Yu, Wei Zheng, Depeng Han, Lei Yang, Zhexuan Jiang, Yewei Cheng, Yazhen Li, Tianbao Huang, Wen Xiong, Xiaoping Huang, Ming Chen, Xiaosan Liu, Meiwei Zhang, Yingjin Huang and Qinghong Zhou

Plants 2026, 15(2), 338; https://doi.org/10.3390/plants15020338 - 22 Jan 2026

Viewed by 313

Abstract

The multidrug and toxic compound extrusion (MATE) protein plays a crucial role in mediating plant responses to aluminum (Al) toxicity. The key candidate gene BnaMATE43b related to Al toxicity stress in rapeseed was identified using GWAS and transcriptome analysis. In this study, the [...] Read more.

The multidrug and toxic compound extrusion (MATE) protein plays a crucial role in mediating plant responses to aluminum (Al) toxicity. The key candidate gene BnaMATE43b related to Al toxicity stress in rapeseed was identified using GWAS and transcriptome analysis. In this study, the BnaMATE43b gene was cloned and functionally characterized in rapeseed. Compared with wild-type rapeseed (WT), the BnaMATE43b overexpression lines (OE) demonstrated stronger aluminum tolerance, specifically manifested in higher relative elongation of taproots (RETs) and relative total root length (RTRL); under Al toxicity stress, the enzyme activities (SOD and POD) and root activity were significantly increased in the OE lines, whereas the MDA content and relative electrical conductivity were reduced in rapeseed root. Further transcriptome analysis of OE-3 showed that the differentially expressed genes (DEGs) were mainly enriched in zeatin biosynthesis (map00908), glucosinolate biosynthesis (map00966), phenylpropanoid biosynthesis (map00940), and ascorbate and aldarate metabolism (map00053). In addition, the yeast cDNA library of rapeseed was constructed, and twenty-two candidate upstream transcription factors (UTFs) of BnaMATE43b were screened; furthermore, four candidate UTFs were obtained through one-on-one interaction validation and luciferase assays, comprising three bHLH transcription factors (BnaA02g28220D, BnaA06g07840D, and BnaA08g24520D) and one ERF transcription factor (BnaA05g23130D). Collectively, these results suggest that BnaMATE43b could improve Al tolerance in rapeseed by mediating antioxidant enzyme activities and the related metabolic pathway, while the obtained UTFs lay the foundation for further analysis of the gene regulatory network under Al toxicity stress. Full article

(This article belongs to the Special Issue Epigenetic Regulation and Molecular Mechanisms in Brassica Crop Improvement)

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18 pages, 2525 KB

Open AccessArticle

More than a Thickener: Xanthan Gum as a Vehicle for the Herbicidal Extract of Saussurea lappa and Its Rheological Characterization

by Shafiu Mustapha, Bryan N. S. Pinto, Ângelo M. L. Denadai and Elson S. Alvarenga

Plants 2026, 15(2), 337; https://doi.org/10.3390/plants15020337 - 22 Jan 2026

Viewed by 552

Abstract

The increasing demand for food is the driving force behind the search for novel, more selective, and less hazardous agrochemicals. Natural products are gaining prominence recently due to the promise of being green agrochemicals, but many natural products are poorly soluble in water, [...] Read more.

The increasing demand for food is the driving force behind the search for novel, more selective, and less hazardous agrochemicals. Natural products are gaining prominence recently due to the promise of being green agrochemicals, but many natural products are poorly soluble in water, which reduces their applicability. In this work, we successfully formulated a water-insoluble Saussurea lappa root extract into a stable aqueous suspension using xanthan gum. The colloidal suspension was characterized by rheology, dynamic light scattering, and zeta potential. The results demonstrated that the suspension is a stable, sprayable, shear-thinning viscoelastic system (weak gel). A series of S. lappa solutions with xanthan gum were prepared and tested against five plant species, observing the inhibitory effect on the shoots and roots. The results were also compared with the commercial herbicide Dual. The S. lappa extract presented results comparable to or even greater than Dual for Lactuca sativa, Cucumis sativus, Brachiaria decumbens, and Bidens pilosa. However, it showed low inhibitory activity for Sorghum bicolor, highlighting its potential for selective weed control. This work illustrates xanthan gum as an effective vehicle for formulating insoluble natural products and demonstrates that S. lappa extract is a promising candidate for developing novel herbicides. Full article

(This article belongs to the Special Issue Advances in Weed Control and Management)

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12 pages, 755 KB

Open AccessArticle

Genotyping-by-Sequencing Reveals Marker-Based Genome Stability in Tetraploid Clementines for Triploid Breeding

by Pablo Aleza, Andres Garcia-Lor, Pierre Mournet, Luis Navarro and Patrick Ollitrault

Plants 2026, 15(2), 336; https://doi.org/10.3390/plants15020336 - 22 Jan 2026

Viewed by 333

Abstract

Tetraploid non-apomictic citrus genotypes are key female parents for 4x × 2x hybridizations aimed at producing seedless triploid hybrids. However, the extent to which different tetraploidization methods affect genome integrity remains insufficiently characterized at a genome-wide scale. In this study, genotyping-by-sequencing (GBS) was [...] Read more.

Tetraploid non-apomictic citrus genotypes are key female parents for 4x × 2x hybridizations aimed at producing seedless triploid hybrids. However, the extent to which different tetraploidization methods affect genome integrity remains insufficiently characterized at a genome-wide scale. In this study, genotyping-by-sequencing (GBS) was used to evaluate marker-based genomic stability in ten tetraploid plants of ‘Clemenules’, ‘Fina’, and ‘Marisol’ clementines obtained via colchicine treatment, in vitro adventitious organogenesis, or somatic cybridization. Diploid parental plants, two haploid plants of ‘Clemenules’ and ‘Fina’ clementines, and one doubled haploid plant of ‘Clemenules’ clementine were included, being the haploid and double haploid essential to resolve allelic phases. After quality filtering, 3333 SNP (Single Nucleotide Polymorphism) markers distributed across the nine citrus chromosomes were identified and used to compare allele dosage patterns along the genome. Across all GBS-covered regions, no major marker-based genomic gains or losses were detected in any tetraploid plant. These results indicate that, at the resolution provided by GBS, all three tetraploidization methods largely preserve chromosome structure, supporting their suitability for citrus triploid breeding programs based on 4x × 2x sexual hybridizations. Full article

(This article belongs to the Special Issue Development and Application of In Vitro Culture Techniques in Plants)

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16 pages, 7594 KB

Open AccessArticle

Rooting Ability of Eucalyptus dunnii Maiden Mini-Cuttings Is Conditioned by Stock Plant Nighttime Temperature

by Matías Nión, Silvia Ross, Jaime González-Tálice, Leopoldo Torres, Sofía Bottarro, Mariana Sotelo-Silveira, Selene Píriz-Pezzutto, Fábio Antônio Antonelo and Arthur Germano Fett-Neto

Plants 2026, 15(2), 335; https://doi.org/10.3390/plants15020335 - 22 Jan 2026

Viewed by 542

Abstract

Clonal propagation often must incorporate heaters to warm stock plants and stabilize growth. This study investigates the impact that different temperature regimes for stock plants have on the rooting capacity of mini-cuttings derived therefrom. Experiments were conducted in growth chambers using two clones [...] Read more.

Clonal propagation often must incorporate heaters to warm stock plants and stabilize growth. This study investigates the impact that different temperature regimes for stock plants have on the rooting capacity of mini-cuttings derived therefrom. Experiments were conducted in growth chambers using two clones of Eucalyptus dunnii Maiden, with clone A’s rooting being moderately better that that of clone B in commercial production. Root primordia differentiation and elongation were faster in clone A than clone B. Stock plants were maintained for one month under two temperature conditions: Δ0 (26/26 °C day/night) and Δ10 (26/16 °C). The main results indicate that rooting significantly decreased with the reduction in nocturnal temperature. Clone A exhibited a 38% reduction in rooting, whereas clone B showed a more pronounced decrease of 65%. In cold nights, soluble carbohydrates at the cutting bases dropped by approximately 25% considering both clones, and overall foliar nutrients also decreased. Cutting base transcript profiles revealed that cold nights decreased the expression of efflux auxin transporter PIN1, increased expression of auxin catabolism-related enzyme DAO, and that expression of auxin nuclear receptor TIR1 remained stable. Fine management of clonal gardens by adjusting thermal conditions can optimize the physiological status of donor plants and enhance the rooting potential and establishment of the derived cuttings. Full article

(This article belongs to the Special Issue Exploring the Formation of Adventitious Roots in Perennial Species: Insights and Perspectives)

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21 pages, 3234 KB

Open AccessArticle

OmicIntegrator: A Simple and Versatile Tool for Meta-Analysis

by Iván Federico Berco Gitman, Cecilia Eugenia María Grossi, Denise Soledad Arico, María Agustina Mazzella and Rita María Ulloa

Plants 2026, 15(2), 334; https://doi.org/10.3390/plants15020334 - 22 Jan 2026

Viewed by 431

Abstract

We developed OmicIntegrator, a broadly adaptable pipeline designed to standardize and integrate publicly available transcriptomic, proteomic, and phosphoproteomic datasets. We applied this workflow to Arabidopsis thaliana etiolated seedlings to identify protein kinases and phosphatases relevant to skotomorphogenic development, a phase during which seedlings [...] Read more.

We developed OmicIntegrator, a broadly adaptable pipeline designed to standardize and integrate publicly available transcriptomic, proteomic, and phosphoproteomic datasets. We applied this workflow to Arabidopsis thaliana etiolated seedlings to identify protein kinases and phosphatases relevant to skotomorphogenic development, a phase during which seedlings rely on tightly regulated signaling networks to ensure survival in darkness. This meta-analysis provided a comprehensive view of gene and protein expression, revealing discrepancies between transcript and protein abundance, suggesting post-transcriptional and post-translational regulation. By integrating multiple datasets, OmicIntegrator reduces experimental bias and enables the detection of phosphorylation events that may be missed in single-condition studies. Distinct phosphorylation patterns were detected across different protein kinase families. Motif enrichment analysis showed a strong overrepresentation of RxxS motifs among phosphosites in protein phosphatases and microtubule-associated proteins, consistent with potential regulation by calcium-dependent protein kinases (CPKs). Across omics layers, CPK3 and CPK9 repeatedly emerged as prominent candidates, highlighting them as priorities for future functional studies in skotomorphogenesis. Overall, our results demonstrate the power of OmicIntegrator as a flexible framework to contextualize signaling landscapes and identify robust patterns and candidate genes and for generating testable hypotheses from integrated multi-omics data in plant developmental biology. Full article

(This article belongs to the Special Issue Technologies, Applications and Innovations in Plant Genetics Research)

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21 pages, 3011 KB

Open AccessArticle

Optimal Nitrogen Application Strategies for Alfalfa Under Different Precipitation Patterns: Balancing Yield, Nitrogen Fertilizer Use Efficiency, and Soil Nitrogen Residue

by Yanbiao Wang, Yuanbo Jiang, Haiyan Li, Boda Li, Jinxi Chen, Minhua Yin, Yanxia Kang, Guangping Qi, Yanlin Ma, Bojie Xie, Haoxiang Jin, Tongjin Wu and Shan Li

Plants 2026, 15(2), 333; https://doi.org/10.3390/plants15020333 - 22 Jan 2026

Viewed by 375

Abstract

Rational nitrogen applications can not only improve nutrient use efficiency, but also reduce environmental pollution caused by nitrogen leaching. To explore reasonable nitrogen application strategies for synergistically enhancing alfalfa production and ecological benefits, this study calibrated and validated the APSIM–Lucerne model based on [...] Read more.

Rational nitrogen applications can not only improve nutrient use efficiency, but also reduce environmental pollution caused by nitrogen leaching. To explore reasonable nitrogen application strategies for synergistically enhancing alfalfa production and ecological benefits, this study calibrated and validated the APSIM–Lucerne model based on field experiments conducted from 2021 to 2023. The effects of nitrogen application levels of 0, 80, 120, 140, 160, 180, 200, and 240 kg/ha on alfalfa yield, soil NO₃⁻–N and NH₄⁺–N residues, and nitrogen use efficiency under dry, normal, and wet years were simulated. The results indicate: (1) The calibrated APSIM–Lucerne model effectively simulates alfalfa yield and soil nitrogen residuals (R² ranging from 0.67 to 0.91, NRMSE between 6.55% and 24.03%). (2) Increased nitrogen application significantly elevates soil nitrogen residue, yet alfalfa yield follows a pattern of initial increase followed by decline, with nitrogen fertilizer use efficiency continuously decreasing. Under identical nitrogen application rates, the wet year type proves more advantageous for achieving high yields, low nitrogen residue, and high nitrogen fertilizer use efficiency. (3) The nitrogen application thresholds for achieving increased alfalfa yields and high efficiency during dry years, normal years, and wet years are 107–140 kg/ha, 135–160 kg/ha, and 150–183 kg/ha, respectively. Full article

(This article belongs to the Special Issue Water and Nitrogen Management in Soil–Crop Systems—4th Edition)

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15 pages, 7983 KB

Open AccessArticle

Antifungal Activity of Acorus calamus Essential Oil Against Rice Blast Fungus Magnaporthe oryzae and Its Composition Characterization

by Shuzhen Deng, Ziyi Wang, Yusi Li, Yiming Liu, Zhiyi Kong, Ge Meng, Saige Jin, Anqi Zeng, Huan Liu and Shengming Liu

Plants 2026, 15(2), 332; https://doi.org/10.3390/plants15020332 - 22 Jan 2026

Viewed by 425

Abstract

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases affecting global rice production. Plant essential oils (EOs) have been considered as a promising green alternative to synthetic fungicides. In this study, the antifungal activities of [...] Read more.

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases affecting global rice production. Plant essential oils (EOs) have been considered as a promising green alternative to synthetic fungicides. In this study, the antifungal activities of five plant EOs—Acorus calamus, Citrus reticulata, Syzygium aromaticum, Paeonia suffruticosa, and Melaleuca viridiflora—against M. oryzae were evaluated using the mycelial growth rate method. Among them, A. calamus EO (ACEO) exhibited the most pronounced inhibitory effect, with an EC₅₀ value of 0.37 μL/mL. It significantly delayed or inhibited conidial germination and appressorium formation. At higher concentrations (≥1 μL/mL), it also caused morphological abnormalities in appressoria. Observations by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that the EO treatment caused hyphal surface wrinkling, cell wall thinning, organelle dissolution, and vacuolation. Pathogenicity tests further confirmed that ACEO reduced the virulence of the fungus remarkably, with nearly complete loss of pathogenicity at a concentration of 1 μL/mL. Finally, ACEO was analyzed using gas chromatography-mass spectrometry (GC-MS). The most abundant constituents identified were β-asarone (19.83%) and isoshyobunone (14.92%). Together, these findings demonstrate that ACEO impairs fungal pathogenicity by disrupting hyphal morphology and cellular integrity, highlighting its potential as an effective and eco-friendly fungicide for controlling rice blast. Full article

(This article belongs to the Section Plant Protection and Biotic Interactions)

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18 pages, 1044 KB

Open AccessReview

Melatonin as a Pre- and Postharvest Tool for Enhancing Fruit Quality

by Pedro Antonio Padilla-González, Fernando Garrido-Auñón, María Emma García-Pastor, Fabián Guillén, María Serrano, Daniel Valero and Vicente Agulló

Plants 2026, 15(2), 331; https://doi.org/10.3390/plants15020331 - 22 Jan 2026

Cited by 2 | Viewed by 621

Abstract

Melatonin (MEL), also known as N-acetyl-5-methoxytryptamine, has been reported in plants as a secondary messenger involved in regulating abiotic stress responses. MEL treatment, either preharvest or postharvest, regulates several physiological and biochemical processes during fruit growth and ripening in horticultural products. These [...] Read more.

Melatonin (MEL), also known as N-acetyl-5-methoxytryptamine, has been reported in plants as a secondary messenger involved in regulating abiotic stress responses. MEL treatment, either preharvest or postharvest, regulates several physiological and biochemical processes during fruit growth and ripening in horticultural products. These include reproductive development, tissue and quality maintenance, delayed senescence, and responses to abiotic stress. Due to its natural origin, low toxicity, and multifunctional regulatory capacity, MEL has recently attracted attention as a promising ‘green preservative’ for sustainable postharvest management. Additionally, MEL coordinates through cross-talk with other plant hormones, such as abscisic acid, ethylene, polyamines, jasmonic acid, γ-aminobutyric acid, salicylic acid, and nitric oxide, to regulate postharvest ripening and senescence. Furthermore, MEL enhances antioxidant systems and improves membrane integrity, thereby alleviating chilling injury and enhancing fruit firmness and colour. Notably, recent evidence highlights the innovative regulatory mechanisms of MEL involving redox homeostasis, hormone signalling reprogramming, and transcriptional modulation of stress-responsive pathways. MEL could therefore be considered an emerging, eco-friendly tool for prolonging the shelf-life of fruit and vegetables and maintaining their quality. This review summarises the mechanisms by which MEL contributes to plant stress resistance by regulating the biosynthesis and metabolism of stress tolerance and improving fruit quality. Full article

(This article belongs to the Special Issue Role of Melatonin in Plant Growth, Metabolism, and Abiotic Stress Tolerance)

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18 pages, 6807 KB

Open AccessArticle

Determining the Critical Period of Continuous Waterlogging in Maize: An Analysis of Physiological, Biochemical, and Transcriptomic Traits

by Denglong Chen, Cong Peng, Zhiming Liu, Wanrong Gu, Fanyun Yao, Lichun Wang, Yujun Cao and Yongjun Wang

Plants 2026, 15(2), 330; https://doi.org/10.3390/plants15020330 - 21 Jan 2026

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Abstract

Waterlogging stress severely limits crop photosynthesis and energy supplies, resulting in significant yield reductions. However, the critical duration of waterlogging stress during the maize jointing stage remains unclear, and the physiological and molecular mechanisms underlying its effects on photosynthetic efficiency and energy synthesis [...] Read more.

Waterlogging stress severely limits crop photosynthesis and energy supplies, resulting in significant yield reductions. However, the critical duration of waterlogging stress during the maize jointing stage remains unclear, and the physiological and molecular mechanisms underlying its effects on photosynthetic efficiency and energy synthesis in maize require further investigation. In this study, we systematically analyzed the responses of physiological traits, transcriptomic profiles, and the yield formation in maize (Zea mays L.) to varying waterlogging durations imposed during the jointing stage, including 0 days (CK), 2 days (F2), 4 days (F4), 6 days (F6), 8 days (F8), and 10 days (F10). Our results indicate that the (1) grain weight (GW) showed no significant difference between F2 and CK. However, the GW in F4, F6, F8, and F10 decreased significantly by 17.49%, 26.45%, 60.24%, and 100.00%, respectively, compared to the CK. (2) Compared with the CK, the malondialdehyde content progressively increased from F4 to F10, while antioxidant enzyme activity gradually decreased. The chlorophyll content declined by 29.93% to 57.38%, and net photosynthetic efficiency decreased by 13.82% to 38.93%. Although the leaf sucrose content in from F4 to F10 gradually decreased, the leaf starch content remained stable in F4 and F6. In contrast, the starch content in F8 and F10 leaves was significantly reduced by 37.55% and 47.60%, respectively, compared with CK. (3) A transcriptomic analysis revealed that during from F2 to F4, genes encoding photosystem I subunit protein, such as PSAD, and the cytochrome b6f complex proteingene PETC were downregulated. At F6, these key genes encoding photosynthetic proteins were upregulated. However, at F8 and F10, their expression was significantly downregulated. Concurrently, genes related to ATP synthesis (e.g., ATPD) as well as starch and sucrose metabolism (e.g., SPP2, SS1) were also downregulated. In summary, when waterlogging stress persists for no longer than 6 days, plants can maintain their starch content to supply energy for growth, thereby ensuring basic developmental needs. When waterlogging persists for more than 6 days, energy synthesis is impaired, and the nutrient transport to the grains is significantly inhibited, ultimately resulting in a substantial reduction in yield. Therefore, 6 days of waterlogging can be considered the critical threshold for significant yield loss in maize during the jointing stage. Full article

(This article belongs to the Special Issue Decoding Plant Stress Responses: An Integration of Physiology and Biochemistry)

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14 pages, 26943 KB

Open AccessArticle

The Influence of Transgenic Insect-Resistance and Herbicide-Tolerance Soybean KM2208-23 on the Rhizosphere Micro-Biome

by Xue Song, Xinyao Xia, Shuke Yang, Chaofeng Hao, Hongwei Sun, Fan Li, Xiaohui Xu, Hongxia Zhang and Xingbo Lu

Plants 2026, 15(2), 329; https://doi.org/10.3390/plants15020329 - 21 Jan 2026

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Abstract

The consequences of stacking multiple insect-resistance and herbicide-tolerance genes, particularly across the entire plant life cycle, remain inadequately understood. This study investigated the impact of stacked-trait transgenic soybeans on rhizosphere microbial communities across five growth stages (pre-sowing, V3, R3, R5, R8). Using 16S [...] Read more.

The consequences of stacking multiple insect-resistance and herbicide-tolerance genes, particularly across the entire plant life cycle, remain inadequately understood. This study investigated the impact of stacked-trait transgenic soybeans on rhizosphere microbial communities across five growth stages (pre-sowing, V3, R3, R5, R8). Using 16S rRNA and ITS sequencing, we compared the rhizosphere microbiome of the transgenic modified soybean (GMO) with its non-transgenic control check (CK). Results showed transient but significant shifts in soil properties (e.g., available nitrogen) and microbial beta diversity during the V3 stages. However, plant developmental stage was the predominant factor shaping microbial succession, with its effect outweighing that of the transgene. No persistent changes in microbial alpha diversity were observed. We conclude that the influence of this stacked-trait soybean on the rhizosphere is growth-stage-specific and represents a minor, recoverable perturbation rather than a sustained ecological impact. These findings contribute to the ecological safety assessment of multi-gene transgenic crops. Full article

(This article belongs to the Special Issue Applications of Bioinformatics in Plant Science)

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39 pages, 3761 KB

Open AccessReview

From Perception to Adaptation: A Comparative Study of Plant Regulatory Networks in Response to Heat and Waterlogging Stress

by Javed Iqbal, Sikandar Amanullah, Chengyue Li, Xiaohui Qin, Pengbo Yu, Xuanyang Chen and Dongliang Qiu

Plants 2026, 15(2), 328; https://doi.org/10.3390/plants15020328 - 21 Jan 2026

Viewed by 481

Abstract

Heat and waterlogging are critical abiotic stresses that threaten crop productivity, especially as climate change intensifies their frequency and severity. While both stresses independently disrupt essential physiological functions such as photosynthesis, respiration, and nutrient uptake, their underlying mechanisms and adaptive strategies exhibit key [...] Read more.

Heat and waterlogging are critical abiotic stresses that threaten crop productivity, especially as climate change intensifies their frequency and severity. While both stresses independently disrupt essential physiological functions such as photosynthesis, respiration, and nutrient uptake, their underlying mechanisms and adaptive strategies exhibit key differences. This review presents a systematic comparison of plant responses to heat and waterlogging stress, focusing on both their shared and distinct impacts on plant physiology, biochemistry, and molecular regulation. We synthesize recent insights from omics technologies, including transcriptomic and metabolomics, to explore regulatory pathways, hormonal crosstalk (e.g., ABA–ethylene interactions), and metabolic shifts (e.g., fermentation vs. chaperone induction) that drive stress tolerance. This comparative analysis similarly demonstrates that effective plant resilience to climate extremes depends on the coordinated optimization of shared stress management hubs, such as antioxidant defense systems and hormonal crosstalk, together with the deployment of stress-specific adaptive strategies, including molecular chaperone induction under heat stress and anaerobic metabolic reprogramming under waterlogging. By integrating convergent and divergent regulatory pathways, this framework provides a mechanistic and conceptual guide for breeding and engineering crops with durable tolerance to multiple, increasingly co-occurring abiotic stresses. Full article

(This article belongs to the Special Issue Biochemical Responses of Horticultural Crops to Abiotic Stresses)

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19 pages, 3652 KB

Open AccessArticle

Spatial Patterns and Diversity of the Genus Agave in the Southern Iberian Peninsula: The Role of Anthropogenic Drivers in the Expansion of Agave americana

by Francisco Guerrero, Víctor Cid-Gaitán, Javier Jurado-Pardeiro, Fernando Ortega and Juan Diego Gilbert

Plants 2026, 15(2), 327; https://doi.org/10.3390/plants15020327 - 21 Jan 2026

Viewed by 494

Abstract

The genus Agave L. is a key component of Mediterranean alien flora, yet its inland distribution in the Iberian Peninsula remains poorly understood. This research integrates exhaustive field surveys with Species Distribution Models (SDMs) to characterize the genus diversity and, specifically, the spatial [...] Read more.

The genus Agave L. is a key component of Mediterranean alien flora, yet its inland distribution in the Iberian Peninsula remains poorly understood. This research integrates exhaustive field surveys with Species Distribution Models (SDMs) to characterize the genus diversity and, specifically, the spatial patterns and environmental niche of Agave americana in the southern Iberian Peninsula (Andalusia). Our results reveal a diversity of 23 taxa, yet crucially, the widespread occurrence of A. americana demonstrated that its actual inland distribution is significantly more extensive than previously recorded. Spatial Point Pattern Analysis (SPPA) revealed a strong aggregated distribution pattern (Clark & Evans R = 0.277; p < 0.001). The MaxEnt Spatial Distribution Model demonstrated robust predictive performance (Mean AUC = 0.770 ± 0.007; Mean TSS = 0.420 ± 0.009). The distribution was primarily driven by elevation range (26.9%) and land use (23.1%), with maximum suitability peaking in anthropized, low-to-intermediate elevation areas. Projections to the broader Andalusian region confirmed high suitability in the Guadalquivir valley and coastal zones, validated by low spatial uncertainty (SD < 0.05 in optimal areas). These findings provide new insights into the biogeography of Agave in the region, emphasizing the significance of anthropogenic drivers within a cultural landscape context. Full article

(This article belongs to the Special Issue Advanced Botanical Research in the Mediterranean Area: Studies in Honor of Prof. Francesco Maria Raimondo on the Occasion of His 80th Birthday)

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14 pages, 2337 KB

Open AccessArticle

Optimizing Daily Light Integral in Seedling Stage Accelerates Heading and Flowering in Wheat Under LED Lighting

by Luming Zhong, Xiang Ji, Jun Liu, Qing Zhou and Dongxian He

Plants 2026, 15(2), 326; https://doi.org/10.3390/plants15020326 - 21 Jan 2026

Viewed by 407

Abstract

Conventional wheat breeding in the field is limited to two generations per year and is susceptible to environmental fluctuations. Speed-breeding in a plant factory offers a solution; however, recommendations for lighting strategies remain limited. To identify the optimal daily light integral (DLI) for [...] Read more.

Conventional wheat breeding in the field is limited to two generations per year and is susceptible to environmental fluctuations. Speed-breeding in a plant factory offers a solution; however, recommendations for lighting strategies remain limited. To identify the optimal daily light integral (DLI) for the seedling stage, we tested three light intensities (300, 500, and 700 μmol m⁻² s⁻¹) and four photoperiods (10, 14, 18, and 22 h d⁻¹), resulting in DLIs ranging from 10.8 to 55.4 mol m⁻² d⁻¹. Our results indicated that an optimal DLI of 39.6 mol m⁻² d⁻¹ was associated with the highest seedling index (0.26) and root-to-shoot ratio (0.42), as well as enhanced photosystem performance. Beyond this DLI, these parameters and shoot biomass plateaued as the DLI increased. Moreover, treating seedlings with this optimal DLI of 39.6 mol m⁻² d⁻¹ (using 500 μmol m⁻² s⁻¹ light intensity and a 22 h d⁻¹ photoperiod) resulted in heading and flowering 5.9 and 7.5 days earlier after transplanting, respectively, than those under the lowest DLI (10.8 mol·m⁻²·d⁻¹). This study established a lighting strategy to produce high-quality seedlings and accelerate heading and flowering, thereby offering a valuable physiological framework for advancing speed-breeding systems in wheat. Full article

(This article belongs to the Topic Sustainability and Innovation in Agriculture for the Food Production of Tomorrow)

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18 pages, 735 KB

Open AccessArticle

Current Knowledge and Utilization of Medicinal Plants and Fungi in Northeastern Croatia

by Ljiljana Krstin, Zorana Katanić, Ivana Turk, Ivana Gajski and Tanja Žuna Pfeiffer

Plants 2026, 15(2), 325; https://doi.org/10.3390/plants15020325 - 21 Jan 2026

Viewed by 591

Abstract

Knowledge related to the use of plants and mushrooms in the Baranja region of Croatia was documented through semi-structured interviews of 105 informants in 12 villages. We found 117 plant species and 7 mushrooms with medicinal uses. Rosaceae, Lamiaceae, and Asteraceae were the [...] Read more.

Knowledge related to the use of plants and mushrooms in the Baranja region of Croatia was documented through semi-structured interviews of 105 informants in 12 villages. We found 117 plant species and 7 mushrooms with medicinal uses. Rosaceae, Lamiaceae, and Asteraceae were the families with the most species, while Sambucus nigra, Chamomilla recutita, and Taraxacum officinale were the most frequently mentioned species. Leaves, fruits, and flowers were the most commonly used plant parts, predominantly prepared as infusions, syrups, and tinctures. Plants were mainly used to treat digestive and respiratory ailments, with the highest informant consensus recorded for ear, eye, and respiratory disorders. The results emphasize the persistence of rich ethnobotanical knowledge in the study area and highlight the importance of preserving this cultural and biological heritage for future generations. Full article

(This article belongs to the Section Plant Systematics, Taxonomy, Nomenclature and Classification)

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34 pages, 2380 KB

Open AccessReview

A Comprehensive Review on Steviol Glycosides: Sources, Properties, Bioactivities, Sensory-Functional Enhancement and Bioproduction Strategies

by Liangzhen Jiang, Xun Zhao, Wei Li, Guiru Tang, Yiming Yuan, Jie Cheng, Jun Hua and Liang Zou

Plants 2026, 15(2), 324; https://doi.org/10.3390/plants15020324 - 21 Jan 2026

Cited by 1 | Viewed by 1289

Abstract

Steviol glycosides (SGs) are high-intensity, zero-calorie natural sweeteners with demonstrated safety and potential health benefits, positioning them as ideal sucrose substitutes for metabolic disorder management. However, their broad application is limited by inherent drawbacks such as bitterness, low solubility, and inefficient production systems. [...] Read more.

Steviol glycosides (SGs) are high-intensity, zero-calorie natural sweeteners with demonstrated safety and potential health benefits, positioning them as ideal sucrose substitutes for metabolic disorder management. However, their broad application is limited by inherent drawbacks such as bitterness, low solubility, and inefficient production systems. This review provides a comprehensive summary of recent advances in SG research, covering their sources, properties, and bioactivities. A particular focus is placed on innovative bioproduction strategies—including enzyme engineering, metabolic pathway optimization, and sustainable extraction techniques. Strategies to overcome these challenges through sensory-function enhancement—including formulation and structural modification—are discussed. Furthermore, it highlights emerging trends like microbial chassis-based production and next-generation sweetener design, providing actionable insights for overcoming industrial bottlenecks. By integrating multidisciplinary advances in bioengineering, sensory science, and sustainable processing, this review offers a forward-looking perspective on the development and application of SGs as functional sweeteners in the global food industry. Full article

(This article belongs to the Special Issue Advances in Plant Natural Products Biosynthesis and Metabolic Engineering—2nd Edition)

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14 pages, 3873 KB

Open AccessArticle

Pyramiding Pita, Pigm, Pi2, and Xa23 to Develop Hybrid Rice with Dual Resistance to Rice Blast and Bacterial Blight

by Siyuan Wu, Xuemei Qin, Jiali Liu, Ju Gao, Lijun Gao, Geng Zhou, Yang Zhou, Tianqi Bai, Chonglie Ma and Fang Liu

Plants 2026, 15(2), 323; https://doi.org/10.3390/plants15020323 - 21 Jan 2026

Viewed by 428

Abstract

Rice blast and bacterial blight cause severe harm to rice production, and the breeding of resistant varieties guarantees the safety of rice production. Meanwhile, multigene pyramiding breeding based on molecular marker-assisted selection is a crucial approach for rice breeding to combat multiple diseases. [...] Read more.

Rice blast and bacterial blight cause severe harm to rice production, and the breeding of resistant varieties guarantees the safety of rice production. Meanwhile, multigene pyramiding breeding based on molecular marker-assisted selection is a crucial approach for rice breeding to combat multiple diseases. This study aimed to develop accurate and efficient PARMS markers for rice blast resistance genes Pita, Pigm, and Pi2, and bacterial blight resistance gene Xa23. A systematic genotyping analysis of the resistant alleles of these 4 genes was performed on 384 major cultivated varieties in production. The results showed that only 5.21% of the varieties harbored more than two resistant alleles simultaneously. Using traditional breeding strategies in combination with the developed PARMS markers, the high-quality three-line male sterile line Ruanfeng A (pyramiding Pita and Pigm) and the strong restorer line Gui 610 (pyramiding Pi2 and Xa23) were bred. Crossing these lines produced a new hybrid rice variety, Ruanfengyou 610. Ruanfengyou 610 pyramids 4 resistance genes (Pita/Pigm/Pi2/Xa23), exhibits resistance to both rice blast and bacterial blight, has prominent heterosis and excellent grain quality, and has strong application potential, which is of great significance for ensuring the safety of rice production. Full article

(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)

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15 pages, 1805 KB

Open AccessArticle

Effect of Light and Cytokinin Modulators on Adventitious Shooting in Melia volkensii Gürke

by Nandini Bhogar Suresh, Lenka Plačková, Karel Doležal and Stefaan P. O. Werbrouck

Plants 2026, 15(2), 322; https://doi.org/10.3390/plants15020322 - 21 Jan 2026

Viewed by 445

Abstract

Adventitious shoot regeneration in woody species is regulated by interactions between plant growth regulators, endogenous hormone metabolism, and environmental cues such as light quality. Here, we investigated the effects of thidiazuron (TDZ) and the cytokinin oxidase/dehydrogenase (CKX) inhibitors INCYDE and phenyladenine (PA), in [...] Read more.

Adventitious shoot regeneration in woody species is regulated by interactions between plant growth regulators, endogenous hormone metabolism, and environmental cues such as light quality. Here, we investigated the effects of thidiazuron (TDZ) and the cytokinin oxidase/dehydrogenase (CKX) inhibitors INCYDE and phenyladenine (PA), in combination with different light spectra, on morphogenesis in Melia volkensii leaf explants. TDZ induced the highest frequencies of callus formation and adventitious shoot regeneration, particularly under white light. INCYDE promoted localized regeneration responses, including activation of dormant meristematic regions in secondary leaf axils, whereas PA showed limited regeneration efficiency. Light quality significantly influenced morphogenesis, with white and blue light favoring organized shoot development, while red and far-red light suppressed shoot regeneration and promoted callus formation. Cytokinin profiling revealed treatment-dependent shifts in endogenous cytokinin composition, most notably in isopentenyladenine (iP)-type cytokinins, which is consistent with altered cytokinin degradation dynamics. Cis-zeatin-type cytokinins were abundant across treatments, likely reflecting regulation associated with in vitro culture conditions. These findings indicate that cytokinin metabolism and light quality jointly influence organogenic competence in Melia volkensii Gürke, providing a physiological basis for optimizing regeneration strategies in woody plants. This study provides the first integrated analysis of cytokinin-modulating compounds and light spectra on adventitious shoot regeneration in Melia volkensii. The findings establish a physiological basis for improving regeneration protocols in recalcitrant woody species and support future biotechnological applications, including genetic improvement and advanced propagation strategies. Full article

(This article belongs to the Special Issue The Role of Tissue Culture in Enhancing Plant Stress Tolerance and Propagation)

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12 pages, 1525 KB

Open AccessArticle

Exploring Chemical Composition of the Aerial Parts of Vernoniastrum migeodii and Anti-Inflammatory Activity of the Compounds

by Morteza Yazdani, Dóra Paróczai, Anita Barta, Katalin Burián and Judit Hohmann

Plants 2026, 15(2), 321; https://doi.org/10.3390/plants15020321 - 21 Jan 2026

Viewed by 309

Abstract

Therapeutic strategies that fine-tune epithelial inflammatory responses are highly sought after in respiratory and mucosal disorders, but few molecules selectively target these pathways. Vernoniastrum migeodii (S. Moore) Isawumi (Asteraceae) represents a chemically promising but understudied source of bioactive small molecules. This study aimed [...] Read more.

Therapeutic strategies that fine-tune epithelial inflammatory responses are highly sought after in respiratory and mucosal disorders, but few molecules selectively target these pathways. Vernoniastrum migeodii (S. Moore) Isawumi (Asteraceae) represents a chemically promising but understudied source of bioactive small molecules. This study aimed to define the metabolite profile of V. migeodii and evaluate the modulation of inflammatory epithelial signaling of the constituents. From the methanolic extract of V. migeodii, five germacranolide sesquiterpenes, vernolide (1), 3′-hydroxylvernolide (2), pectorolide (3), 4′-hydroxypectorolide-14-O-acetate (4) and 4′-hydroxypectorolide (5), together with (6S,9R)-vomifoliol (6), eucarvone (7), luteolin (8), and luteolin-7-O-glucoside (9) were isolated by multiple chromatographic separations. The structures were determined by comprehensive 1D and 2D NMR spectroscopy. Isolated compounds 1 to 9 together with previously reported steroids (10–17) and tripeptide (18) were evaluated in LPS-activated A549 cells by quantitative PCR for interleukin-6 (IL6), interleukin-1β (IL1β), and prostaglandin-endoperoxide synthase 2 (PTGS2) and by enzyme-linked immunosorbent assay (ELISA) for IL-6 and IL-8. Compounds 2, 7, steroids 10–17 and aurantiamide acetate (18) reduced IL6 mRNA relative to the LPS control, while (6S,9R)-vomifoliol (6) increased IL-6 and elevated IL-8. In the assay IL1β and PTGS2 transcripts were not significantly altered. These findings highlight the potential of V. migeodii metabolites as modulators of epithelial inflammatory pathways. Combining chemical and biological evidence provides a clear basis for structure–activity- and pathway-focused studies. Full article

(This article belongs to the Special Issue Phytochemical Characterization and Bioactivity of Medicinal Plants: From Analytical Techniques to Therapeutic Potential)

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15 pages, 6022 KB

Open AccessPerspective

A Multidimensional Approach to Cereal Caryopsis Development: Insights into Adlay (Coix lacryma-jobi L.) and Emerging Applications

by Xiaoyu Yang, Jian Zhang, Maohong Ao, Jing Lei and Chenglong Yang

Plants 2026, 15(2), 320; https://doi.org/10.3390/plants15020320 - 21 Jan 2026

Viewed by 416

Abstract

Adlay (Coix lacryma-jobi L.) stands out as a vital health-promoting cereal due to its dual nutritional and medicinal properties; however, it remains significantly underdeveloped compared to major crops. The lack of mechanistic understanding of its caryopsis development and trait formation severely constrains [...] Read more.

Adlay (Coix lacryma-jobi L.) stands out as a vital health-promoting cereal due to its dual nutritional and medicinal properties; however, it remains significantly underdeveloped compared to major crops. The lack of mechanistic understanding of its caryopsis development and trait formation severely constrains targeted genetic improvement. While transformative technologies, specifically micro-computed tomography (micro-CT) imaging combined with AI-assisted analysis (e.g., Segment Anything Model (SAM)) and multi-omics approaches, have been successfully applied to unravel the structural and physiological complexities of model cereals, their systematic adoption in adlay research remains fragmented. Going beyond a traditional synthesis of these methodologies, this article proposes a novel, multidimensional framework specifically designed for adlay. This forward-looking strategy integrates high-resolution 3D phenotyping with spatial multi-omics data to bridge the gap between macroscopic caryopsis architecture and microscopic metabolic accumulation. By offering a precise digital solution to elucidate adlay’s unique developmental mechanisms, the proposed framework aims to accelerate precision breeding and advance the scientific modernization of this promising underutilized crop. Full article

(This article belongs to the Special Issue AI-Driven Machine Vision Technologies in Plant Science)

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42 pages, 1490 KB

Open AccessReview

A Review on Sasa quelpaertensis’s Phytochemical Profiles and Pharmacological Activities

by Varun Jaiswal and Hae-Jeung Lee

Plants 2026, 15(2), 319; https://doi.org/10.3390/plants15020319 - 21 Jan 2026

Viewed by 630

Abstract

Sasa quelpaertensis, a multipurpose bamboo plant endemic to Jeju Island in South Korea, is used by the population in traditional medicine for its anti-inflammatory, anti-diabetic, anti-gastritis, and diuretic activities. Studies have shown the potential of S. quelpaertensis against various diseases; its effects [...] Read more.

Sasa quelpaertensis, a multipurpose bamboo plant endemic to Jeju Island in South Korea, is used by the population in traditional medicine for its anti-inflammatory, anti-diabetic, anti-gastritis, and diuretic activities. Studies have shown the potential of S. quelpaertensis against various diseases; its effects include anticancer, anti-obesity, anti-diabetic, anti-inflammatory, antibacterial, antiviral, antioxidant, antidepressant, immunomodulating, and hepatoprotective effects. Several bioactive phytochemicals, including p-coumaric acid, tricin, naringenin, and vanillic acid, have been identified in S. quelpaertensis, further emphasizing its pharmacological potential. Molecular studies have identified crucial pharmacological targets of S. quelpaertensis, such as adenosine monophosphate-activated protein kinase (AMPK) and nuclear factor kappa B (NF-κB) signaling. The major challenges are that most pharmacological activities have been observed only in the preclinical stage, and that a compilation of its phytochemicals and pharmacological activities is missing from the literature. The studies with incomplete extract characterization or standardization limit the comparability across studies. Identification of active phytochemicals for specific activities and large-scale clinical trials for the majority of pharmacological effects are suggested. This review not only compiles the phytochemicals and pharmacological properties of S. quelpaertensis but also highlights current gaps and proposes solutions for its development as a therapeutic agent and/or supplement against major diseases. Full article

(This article belongs to the Special Issue Bio-Active Compounds in Horticultural Plants—2nd Edition)

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19 pages, 2842 KB

Open AccessArticle

Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7

by Aoga Li, Chongyang Yao, Ting Yan, Xiaomin Hao, Dongying Geng, Qi Zhang, Hui Li, Wenquan Bao and Yue Bai

Plants 2026, 15(2), 318; https://doi.org/10.3390/plants15020318 - 21 Jan 2026

Viewed by 512

Abstract

GmSRC7 is a broad-spectrum antiviral R gene from soybean, but its downstream and functionally related genes remain unclear. Virus-induced gene silencing (VIGS) assays in Nicotiana benthamiana (Nb) showed that suppression of several gene families—WRKY transcription factors, chaperones, ethylene pathway components, MAPK [...] Read more.

GmSRC7 is a broad-spectrum antiviral R gene from soybean, but its downstream and functionally related genes remain unclear. Virus-induced gene silencing (VIGS) assays in Nicotiana benthamiana (Nb) showed that suppression of several gene families—WRKY transcription factors, chaperones, ethylene pathway components, MAPK cascade elements, salicylic acid (SA) signaling genes, calcium-dependent protein kinases, nuclear migration proteins, RNA replication-related genes, and immune regulators—consistently weakened GmSRC7-mediated resistance to Soybean Mosaic Virus (SMV) and Tobacco Mosaic Virus (TMV). Targeted silencing of four regulatory genes—NbEDS1, NbARF1, NbSGT1, and NbCOI1—markedly enhanced GmSRC7-mediated resistance to SMV and TMV in our experiments. Silencing the serine/threonine kinase gene NbPBS1 increased GmSRC7-conferred resistance to SMV but did not significantly alter its resistance to TMV. Transient expression assays showed that NbARF1, NbSGT1, and NbCOI1 antagonize GmSRC7-mediated defense against SMV and TMV, whereas NbPBS1 specifically suppresses anti-SMV activity without affecting TMV resistance. Transient overexpression of SA-degrading enzymes (AtS3H, AtS5H, and NahG) significantly reduced GmSRC7-conferred resistance to SMV, indicating that SA is essential for this R protein-mediated defense. Genes were also grouped by immune pathways and function: co-expression of chaperone family genes inhibited GmSRC7 activity against SMV and TMV, while co-expression of WRKY family genes enhanced anti-SMV activity of GmSRC7. Finally, transient silencing of soybean genes GmEDS1, GmSGT1-1, GmSGT1-2, GmJAR1, and GmSGS3 compromised GmSRC7-mediated resistance to SMV. Full article

(This article belongs to the Special Issue Advances in Plant Molecular Biology and Gene Function)

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37 pages, 3289 KB

Open AccessReview

Essential Oil of Prangos ferulacea (L.) Lindl.: Chemistry and Bioactivities

by Mijat Božović, Vanja Tadić, Milan Mladenović and Rino Ragno

Plants 2026, 15(2), 317; https://doi.org/10.3390/plants15020317 - 21 Jan 2026

Viewed by 530

Abstract

Prangos ferulacea (L.) Lindl. (Apiaceae) is an orophilous species with notable traditional uses, particularly across the broader Middle East region. Over the past 50 years, research on its essential oil has revealed the existence of several chemotypes. In addition to its chemical composition, [...] Read more.

Prangos ferulacea (L.) Lindl. (Apiaceae) is an orophilous species with notable traditional uses, particularly across the broader Middle East region. Over the past 50 years, research on its essential oil has revealed the existence of several chemotypes. In addition to its chemical composition, there is also data on the biological activities of the essential oil. Among these activities, the most extensively studied are its antimicrobial and, to a lesser extent, antioxidant properties. Recent findings suggest the presence of additional biological effects, including cytotoxic, insecticidal, and phytotoxic effects. This review summarizes current knowledge and provides a foundation for future research, including more in-depth chemical and chemotaxonomic analyses, as well as exploration of the full therapeutic potential of this species. Full article

(This article belongs to the Special Issue Plant-Derived Natural Products: Extraction Methods, Chemistry and Bioactivities)

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28 pages, 2892 KB

Open AccessArticle

Foliar Application of Silicon and Sulfur Modifies Grain Mineral Composition of Spring Oats ( Avena sativa L.) Under Contrasting Seasonal Drought Conditions

by Bekir Bytyqi, Fanni Zsuzsa Forgács, Anteneh Agezew Melash, István Csaba Virág, József Csajbók, Ebenezer Ayew Appiah and Erika Tünde Kutasy

Plants 2026, 15(2), 316; https://doi.org/10.3390/plants15020316 - 21 Jan 2026

Viewed by 557

Abstract

This study evaluated the effects of foliar silicon (Si) and sulfur (S) applications under contrasting climatic conditions on macro- and micronutrient accumulation in oat grain. The three-year field experiment (2022–2024) was conducted in Debrecen, Hungary, using a randomized complete block design (RCBD)with three [...] Read more.

This study evaluated the effects of foliar silicon (Si) and sulfur (S) applications under contrasting climatic conditions on macro- and micronutrient accumulation in oat grain. The three-year field experiment (2022–2024) was conducted in Debrecen, Hungary, using a randomized complete block design (RCBD)with three replications. Grain samples were analyzed for macroelements (K, P, S, Mg, Ca) and micronutrients (Na, Si, Fe, Mn, Cu). Environmental conditions markedly influenced nutrient accumulation. Severe drought promoted the highest concentrations of K, S, and Mg, while mild drought significantly increased the accumulation of P, Ca, Si, Fe, and Cu contents. Moderate drought favored Na accumulation. Foliar S application under relatively favorable water supply significantly enhanced the concentration of all measured elements, with the strongest response observed for Cu (+47.4% compared with the control) and the weakest for Mg (8.5%). In contrast, Si application alone had only limited or negative effects, particularly under severe drought, where it reduced K (6.4%), S (2.4%), and Ca (13%) concentrations, despite increased Si accumulation in the grain. During drought stress, however, the combined Si + S treatment significantly increased the grain macro- and micronutrient concentrations. Among the tested genotypes, ‘Mv Pehely’ exhibited the highest macronutrient accumulation, while ‘GK Kormorán’ and ‘Mv Pehely’ showed superior micronutrient accumulation. ‘GK Pillangó’ and ‘Mv Szellő’ showed consistently lower nutrient contents. These results highlight the importance of genotype × environment × nutrient management strategies for improving nutrient composition in oat grain. Full article

(This article belongs to the Special Issue Nutrient Management for Crop Production and Quality)

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20 pages, 20102 KB

Open AccessArticle

Influence of Alpine Forest Types on Soil Microbial Diversity and Soil Quality

by Shuang Ji, Xunxun Qiu, Huichun Xie, Zhiqiang Dong and Hongye Li

Plants 2026, 15(2), 315; https://doi.org/10.3390/plants15020315 - 21 Jan 2026

Viewed by 358

Abstract

Alpine forests are key regulators of soil biogeochemical cycles, yet the extent to which forest type constrains soil microbial diversity and soil quality in high-elevation regions remains insufficiently resolved. Here, we assessed how contrasting alpine forest types influence the taxonomic composition and diversity [...] Read more.

Alpine forests are key regulators of soil biogeochemical cycles, yet the extent to which forest type constrains soil microbial diversity and soil quality in high-elevation regions remains insufficiently resolved. Here, we assessed how contrasting alpine forest types influence the taxonomic composition and diversity of soil microbial communities, identified the dominant environmental drivers, and evaluated soil quality along the southern slope of the Qilian Mountains. Six forest types were examined, including four monospecific stands (Picea crassifolia, QQ; Betula spp., HS; Juniperus przewalskii, YB; and Pinus tabuliformis, YS) and two mixed formations (mixed conifer–broadleaf, ZKHJ; and mixed broadleaved, KKHJ). Bacterial and fungal communities were characterized using Illumina high-throughput sequencing, while structural equation modeling (SEM) was used to identify primary drivers of diversity and principal component analysis (PCA) was applied to construct the minimum data set (MDS) for soil quality evaluation. Mixed forests consistently exhibited higher bacterial and fungal alpha diversity than pure stands. Environmental gradients were the strongest positive drivers of microbial diversity, whereas soil chemical properties and vegetation-related biotic factors exerted partially negative effects. Soil quality index (SQI) values ranked as follows: KKHJ (0.55) > ZKHJ (0.49) > YB (0.48) > HS (0.46) > YS (0.44) > QQ (0.43). The mixed broadleaved forest reached Grade IV (upper-intermediate level) soil quality, whereas the other forest types were classified as Grade III (intermediate). Mixed forests showed stronger capacities for organic matter accumulation and nutrient retention. These findings indicate that promoting mixed forest stands is critical for improving soil structure, nutrient retention, and microbial diversity in this alpine region. Accordingly, forest management should prioritize the development of mixed forests to enhance overall soil quality. Full article

(This article belongs to the Special Issue Plant-Soil-Microbe Interactions for the Health of Agroforest Ecosystems)

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16 pages, 6178 KB

Open AccessArticle

Phenotypic and Agromorphological Diversity Reveals Detailed Information About Accessions with Productive Potential for Cotton (Gossypium barbadense L.) in Northeastern Peru

by Deyvis Córdova-Sinarahua, Susan Linares-Huapaya and Emma Imelda Manco-Céspedes

Plants 2026, 15(2), 314; https://doi.org/10.3390/plants15020314 - 21 Jan 2026

Viewed by 539

Abstract

Cotton (Gossypium barbadense L.) is a crop of great economic importance, as the superior quality of its fiber is highly valued worldwide. The objective of this research was to evaluate the agromorphological diversity of cotton germplasm using both qualitative and quantitative descriptors. [...] Read more.

Cotton (Gossypium barbadense L.) is a crop of great economic importance, as the superior quality of its fiber is highly valued worldwide. The objective of this research was to evaluate the agromorphological diversity of cotton germplasm using both qualitative and quantitative descriptors. A combination of univariate and multivariate statistical analyses was applied to identify promising accessions. The coefficients of variation (GCV and PCV) and high heritability estimates observed for descriptors such as the number of sympodial branches, fiber weight, and number of seeds per capsule confirm genetic control, ensuring the effectiveness of selection in future breeding programs. Correlation analysis revealed a strong positive relationship between lint yield and number of bolls per plant (0.893). Furthermore, principal component analysis indicated that accessions PER1010536, PER1010538, PER1010543, and PER1010547 were associated with high yield and early-maturity traits. Furthermore, multiple correspondence analysis and mixed data factor analysis demonstrated that the observed variability also depends on qualitative traits such as petal spot and bract color, supporting the concept of a complex genetic architecture. These findings provide a solid basis for the development of new cotton cultivars with improved productivity. Full article

(This article belongs to the Special Issue Genetic Diversity and Molecular Marker Development for Cotton Breeding and Crop Improvement)

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18 pages, 4155 KB

Open AccessArticle

Functional Analysis of OsDRP2B in Rice Root Development

by Lihuiying Jia, Zhiqiang Guo, Yunyan Hua, Qi Zhu, Fengyi Zhou, Qiuping Li, Xu Li, Mengsha Li, Mengting Wang, Yujie Chen, Xiaofei Wang, Tao Ma and Wona Ding

Plants 2026, 15(2), 313; https://doi.org/10.3390/plants15020313 - 21 Jan 2026

Viewed by 538

Abstract

Rice (Oryza sativa L.) root system plays a critical role in water and nutrient uptake, influencing overall plant growth and crop yield. In this study, we characterized the Osdrp2b mutant, which exhibits a short-root phenotype and was identified through map-based cloning. The [...] Read more.

Rice (Oryza sativa L.) root system plays a critical role in water and nutrient uptake, influencing overall plant growth and crop yield. In this study, we characterized the Osdrp2b mutant, which exhibits a short-root phenotype and was identified through map-based cloning. The Osdrp2b mutation was traced to the gene encoding a dynamin-related protein, and the mutant displayed reduced cell elongation and impaired cell division in the root tip. Further analysis revealed that ROS (reactive oxygen species) accumulation was elevated in the mutant roots, and treatment with ROS inhibitors restored root elongation in the Osdrp2b mutant, indicating that altered ROS homeostasis is associated with the phenotype. Transcriptomic analysis highlighted the differential expression of genes involved in cell wall organization and hydrogen peroxide catabolism. Agronomic evaluations of the Osdrp2b mutant demonstrated compromised shoot growth, reduced tiller number, and lower seed setting rates, indicating the impact of the mutation on rice yield. Overall, these results suggest that OsDRP2B is involved in regulating root growth, potentially through effects on ROS homeostasis and associated signaling networks. These findings provide a basis for future studies on improving rice root development and agronomic performance. Full article

(This article belongs to the Section Crop Physiology and Crop Production)

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17 pages, 1748 KB

Open AccessReview

Next-Generation Biopesticides for the Control of Fungal Plant Pathogens

by Younes Rezaee Danesh, Nurhan Keskin, Solmaz Najafi, Harlene Hatterman-Valenti and Ozkan Kaya

Plants 2026, 15(2), 312; https://doi.org/10.3390/plants15020312 - 20 Jan 2026

Cited by 1 | Viewed by 1004

Abstract

This review explores the innovative approaches in the development of next-generation biopesticides, focusing on molecular and microbial strategies for effective control of fungal plant pathogens. As agricultural practices increasingly seek sustainable solutions to combat plant diseases, biopesticides have emerged as a promising alternative [...] Read more.

This review explores the innovative approaches in the development of next-generation biopesticides, focusing on molecular and microbial strategies for effective control of fungal plant pathogens. As agricultural practices increasingly seek sustainable solutions to combat plant diseases, biopesticides have emerged as a promising alternative to chemical pesticides, offering reduced environmental impact and enhanced safety for non-target organisms. The review begins by outlining the critical role of fungal pathogens in global agriculture, emphasizing the need for novel control methods that can mitigate their detrimental effects on crop yields. Key molecular strategies discussed include the use of genetic engineering to enhance the efficacy of biopesticides, the application of RNA interference (RNAi) techniques to target specific fungal genes, and the development of bioactive compounds derived from natural sources. Additionally, this review highlights the potential of microbial agents, such as beneficial bacteria and fungi, in establishing biocontrol mechanisms that promote plant health and resilience. Through a comprehensive review of recent studies and advancements in the field, this manuscript illustrates how integrating molecular and microbial strategies can lead to the development of effective biopesticides tailored to combat specific fungal threats. The implications of these strategies for sustainable agriculture are discussed, alongside the challenges and future directions for research and implementation. Ultimately, this review aims to provide a thorough understanding of the transformative potential of next-generation biopesticides in the fight against fungal plant pathogens, contributing to the broader goal of sustainable food production. Full article

(This article belongs to the Special Issue Biopesticides for Plant Protection)

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17 pages, 4431 KB

Open AccessArticle

Exogenous Methyl Jasmonate Mediates Secondary Metabolic Reprogramming to Enhance Resistance in Tea Plants

by Jie Liu, Zaifa Shu, Xinyan Lan, Dayun Zhou, Huiting Yang, Huijuan Zhou, Qingyong Ji, Limin Chen and Weizhong He

Plants 2026, 15(2), 311; https://doi.org/10.3390/plants15020311 - 20 Jan 2026

Viewed by 492

Abstract

Tea plants are frequently threatened by insect pests, resulting in substantial yield and quality losses. Methyl jasmonate (MeJA) is a key defense signaling molecule in plants; however, its integrated effects on tea plant growth, resistance, and quality-related traits remain poorly understood. In this [...] Read more.

Tea plants are frequently threatened by insect pests, resulting in substantial yield and quality losses. Methyl jasmonate (MeJA) is a key defense signaling molecule in plants; however, its integrated effects on tea plant growth, resistance, and quality-related traits remain poorly understood. In this study, field experiments were conducted to evaluate the effects of exogenous MeJA at different concentration (0.02–20 mM) on growth traits, quality components, and resistance to the tea green leafhopper and tea orange gall mite in Camellia sinensis ‘Zhongcha 108’, and transcriptome analysis was further integrated to elucidate the underlying molecular mechanisms. The results showed that appropriate MeJA concentrations (0.2–2 mM) significantly optimized bud morphology, characterized by shortened internodes, thicker stems, and reduced leaf insertion angles. Importantly, these treatments did not significantly alter the measured quality-related biochemical components, such as free amino acids and soluble sugars, within the evaluated time frame. Collectively, this study provides the first field-based evidence defining an effective MeJA concentration window that balances pest resistance induction, growth modulation, and processing suitability for flat-type green tea, offering practical guidance for the rational application of MeJA in tea plantation management. Full article

(This article belongs to the Special Issue Metabolic Pathways and Hormonal Regulation in Plant Defense and Growth)

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Plants, Volume 15, Issue 2 (January-2 2026) – 168 articles

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