The Turnera Style S-Locus Gene TsBAHD Possesses Brassinosteroid-Inactivating Activity When Expressed in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Expression of TsBAHD in A. Thaliana Induces Dwarfism
2.2. Assessment of Expression of BR Biosynthesis and Inactivating Genes
2.3. Response of TsBAHD Expressing Hypocotyls to Exogenous BR in Light and Dark
3. Discussion
4. Materials and Methods
4.1. Plasmid Construction and Generation of Transgenic Lines
4.2. Plant Growth Conditions and Transformation Screening
4.3. RNA Extraction, Reverse Transcription, and RT-qPCR Analyses
4.4. BL Dose Response Growth and Hypocotyl Measurements
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Matzke, C.M.; Shore, J.S.; Neff, M.M.; McCubbin, A.G. The Turnera Style S-Locus Gene TsBAHD Possesses Brassinosteroid-Inactivating Activity When Expressed in Arabidopsis thaliana. Plants 2020, 9, 1566. https://doi.org/10.3390/plants9111566
Matzke CM, Shore JS, Neff MM, McCubbin AG. The Turnera Style S-Locus Gene TsBAHD Possesses Brassinosteroid-Inactivating Activity When Expressed in Arabidopsis thaliana. Plants. 2020; 9(11):1566. https://doi.org/10.3390/plants9111566
Chicago/Turabian StyleMatzke, Courtney M., Joel S. Shore, Michael M. Neff, and Andrew G. McCubbin. 2020. "The Turnera Style S-Locus Gene TsBAHD Possesses Brassinosteroid-Inactivating Activity When Expressed in Arabidopsis thaliana" Plants 9, no. 11: 1566. https://doi.org/10.3390/plants9111566