The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile
Abstract
:1. Introduction
2. Results
2.1. The Effect of Different Extraction Protocols on Antioxidant Activity, Bioactive Compounds, and Sugar Content in Brassica
2.2. Multivariate Analysis of the Data Obtained by Different Extraction Protocols
2.3. Correlations between Bioactive Compounds, Antioxidant Activity, and Sugar Content in Brassica Extracts
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Hot Methanol Extraction
4.3. Cold Methanol Extraction
4.4. Determination of Total Antioxidant Activity
4.5. Determination of Total Glucosinolates and Total Phenolic Content
4.6. Sugar Analysis by HPLC
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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TGls | TPC | DPPH | FRAP | Sucrose | Glucose | Fructose | |
---|---|---|---|---|---|---|---|
TGls | 1.00 | ||||||
TPC | 0.14 1 | 1.00 | |||||
DPPH | 0.06 | −0.09 | 1.00 | ||||
FRAP | 0.31 | 0.78 | 0.09 | 1.00 | |||
Sucrose | −0.08 | −0.44 | 0.27 | −0.23 | 1.00 | ||
Glucose | −0.13 | −0.65 | 0.26 | −0.41 | 0.47 | 1.00 | |
Fructose | −0.01 | −0.23 | 0.12 | 0.09 | 0.55 | 0.43 | 1.00 |
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Major, N.; Prekalj, B.; Perković, J.; Ban, D.; Užila, Z.; Ban, S.G. The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile. Plants 2020, 9, 1792. https://doi.org/10.3390/plants9121792
Major N, Prekalj B, Perković J, Ban D, Užila Z, Ban SG. The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile. Plants. 2020; 9(12):1792. https://doi.org/10.3390/plants9121792
Chicago/Turabian StyleMajor, Nikola, Bernard Prekalj, Josipa Perković, Dean Ban, Zoran Užila, and Smiljana Goreta Ban. 2020. "The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile" Plants 9, no. 12: 1792. https://doi.org/10.3390/plants9121792