Genome-Wide Association Analysis Identifies Candidate Genes Regulating Seed Number per Silique in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Seed Number Per Silique Vary Significantly Among Arabidopsis Accessions
2.2. 38 SNP-SNS Associations Indentified in GWAS
2.3. PIN3 Is a Positive Regulator of SNS
2.4. Overexpression of PIN3 Increases SNS
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Phenotypic Analysis of Seed Number
4.3. Genome-Wide Association Study
4.4. Vector Construction and Transformation Processes of Overexpression and Complementation Lines
4.5. Identification of Mutants, Overexpression and Complementary Lines
4.6. Quantitative Real-time RT-PCR
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
GWAS | Genome-wide association analysis |
SNS | Seed number per silique |
SD | Seed density |
STK | SEEDSTICK |
CMM | Carpel margin meristem |
SNP | Single nucleotide polymorphisms |
OD | Ovule density |
TGW | Thousand grain weight |
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Jiang, H.-L.; Hong, J.; Jiang, Y.-T.; Yu, S.-X.; Zhang, Y.-J.; Shi, J.-X.; Lin, W.-H. Genome-Wide Association Analysis Identifies Candidate Genes Regulating Seed Number per Silique in Arabidopsis thaliana. Plants 2020, 9, 585. https://doi.org/10.3390/plants9050585
Jiang H-L, Hong J, Jiang Y-T, Yu S-X, Zhang Y-J, Shi J-X, Lin W-H. Genome-Wide Association Analysis Identifies Candidate Genes Regulating Seed Number per Silique in Arabidopsis thaliana. Plants. 2020; 9(5):585. https://doi.org/10.3390/plants9050585
Chicago/Turabian StyleJiang, Huan-Li, Jun Hong, Yu-Tong Jiang, Shi-Xia Yu, Yan-Jie Zhang, Jian-Xin Shi, and Wen-Hui Lin. 2020. "Genome-Wide Association Analysis Identifies Candidate Genes Regulating Seed Number per Silique in Arabidopsis thaliana" Plants 9, no. 5: 585. https://doi.org/10.3390/plants9050585