Nitrate Promotes Germination Under Inhibition by NaCl or High Concentration of Glucose
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Seed Germination Assays
2.2. RNA Extraction
2.3. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
2.4. Semi-Quantitative RT-PCR
2.5. Statistical Analysis
3. Results
3.1. Germination in the Presence of NaCl or Mannitol
3.2. Nitrate Promotes Germination Inhibited by NaCl or High Concentrations of Glucose
3.3. Gene Expression in Seeds Imbibed with NaCl, Glucose, ABA, and Mannitol
3.4. Gene Expression in Seeds Imbibed With or Without KNO3 in the Presence of NaCl, Glucose, ABA, or Mannitol
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ikeya, S.; Aoyanagi, T.; Ishizuka, I.; Takeuchi, A.; Kozaki, A. Nitrate Promotes Germination Under Inhibition by NaCl or High Concentration of Glucose. Plants 2020, 9, 707. https://doi.org/10.3390/plants9060707
Ikeya S, Aoyanagi T, Ishizuka I, Takeuchi A, Kozaki A. Nitrate Promotes Germination Under Inhibition by NaCl or High Concentration of Glucose. Plants. 2020; 9(6):707. https://doi.org/10.3390/plants9060707
Chicago/Turabian StyleIkeya, Shun, Takuya Aoyanagi, Imari Ishizuka, Ayano Takeuchi, and Akiko Kozaki. 2020. "Nitrate Promotes Germination Under Inhibition by NaCl or High Concentration of Glucose" Plants 9, no. 6: 707. https://doi.org/10.3390/plants9060707