Methylene Blue Reduces Electroretinogram Distortion and Ganglion Cell Death in a Rat Model of Glaucoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ocular Hypertension Glaucoma Model Using Episcleral Vein Cauterization
2.2. Scotopic Electroretinography (ERG)
2.3. Pattern Electroretinography (PERG)
2.4. Histology and Morphological Evaluation
2.5. Statistical Analysis
3. Results
3.1. Surgery and Treatment Groups
3.2. Methylene Blue Restores Electroretinogram Patterns
3.2.1. Scotopic Electroretinography
3.2.2. Oscillatory Potentials
3.2.3. Pattern Electroretinography
3.3. Methylene Blue Prevents Loss of RGC and IR Thickness
3.3.1. Peripheral Retina Morphology
3.3.2. Central Retina Morphology
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nakamura, R.; Ciranna, N.S.; Fernández, J.C.; Peláez, R.; Pérez-Sala, Á.; Bobadilla, M.; López-Costa, J.J.; Loidl, C.F.; Martínez, A.; Rey-Funes, M. Methylene Blue Reduces Electroretinogram Distortion and Ganglion Cell Death in a Rat Model of Glaucoma. Biomedicines 2024, 12, 1983. https://doi.org/10.3390/biomedicines12091983
Nakamura R, Ciranna NS, Fernández JC, Peláez R, Pérez-Sala Á, Bobadilla M, López-Costa JJ, Loidl CF, Martínez A, Rey-Funes M. Methylene Blue Reduces Electroretinogram Distortion and Ganglion Cell Death in a Rat Model of Glaucoma. Biomedicines. 2024; 12(9):1983. https://doi.org/10.3390/biomedicines12091983
Chicago/Turabian StyleNakamura, Ronan, Nicolás S. Ciranna, Juan C. Fernández, Rafael Peláez, Álvaro Pérez-Sala, Miriam Bobadilla, Juan J. López-Costa, César F. Loidl, Alfredo Martínez, and Manuel Rey-Funes. 2024. "Methylene Blue Reduces Electroretinogram Distortion and Ganglion Cell Death in a Rat Model of Glaucoma" Biomedicines 12, no. 9: 1983. https://doi.org/10.3390/biomedicines12091983