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Article

Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar

by
Markéta Jarošová
1,
Pavel Roudnický
2,
Jan Bárta
1,*,
Zbyněk Zdráhal
2,
Veronika Bártová
1,
Adéla Stupková
1,
František Lorenc
3,
Marie Bjelková
4,
Jan Kyselka
5,
Eva Jarošová
1,
Jan Bedrníček
3 and
Andrea Bohatá
1
1
Department of Plant Production, Faculty of Agriculture and Technology, University of South Bohemia, Na Sádkách 1780, 370 05 České Budějovice, Czech Republic
2
Mendel Centre of Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
3
Department of Food Biotechnology and Agricultural Products Quality, Faculty of Agriculture and Technology, University of South Bohemia, Studentská 1668, 370 05 České Budějovice, Czech Republic
4
Department of Legumes and Technical Crops, Agritec Plant Research Ltd., Zemědělská 2520/16, 787 01 Šumperk, Czech Republic
5
Department of Dairy, Fat and Cosmetics, University of Chemistry and Technology, Technická 5, 166 28 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Foods 2024, 13(9), 1288; https://doi.org/10.3390/foods13091288
Submission received: 10 March 2024 / Revised: 15 April 2024 / Accepted: 17 April 2024 / Published: 23 April 2024
(This article belongs to the Special Issue Plant Extracts as Functional Food Ingredients)
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Abstract

The research is focused on the quantitative evaluation of the flaxseed (Linum usitatissimum L.) proteome at the level of seed cake (SC), fine flour—sieved a fraction below 250 µm (FF)—and protein concentrate (PC). The evaluation was performed on three oilseed flax cultivars (Agriol, Raciol, and Libra) with different levels of α-linolenic acid content using LC-MS/MS (shotgun proteomics) analysis, which was finalized by database searching using the NCBI protein database for Linum usitatissimum and related species. A total of 2560 protein groups (PGs) were identified, and their relative abundance was calculated. A set of 33 quantitatively most significant PGs was selected for further characterization. The selected PGs were divided into four classes—seed storage proteins (11S globulins and conlinins), oleosins, defense- and stress-related proteins, and other major proteins (mainly including enzymes). Seed storage proteins were found to be the most abundant proteins. Specifically, 11S globulins accounted for 41–44% of SC proteins, 40–46% of FF proteins, and 72–84% of PC proteins, depending on the cultivar. Conlinins (2S albumins) were the most abundant in FF, ranging from 10 to 13% (depending on cultivar). The second most important class from the point of relative abundance was oleosins, which were represented in SC and FF in the range of 2.1–3.8%, but only 0.36–1.20% in PC. Surprisingly, a relatively high abundance of chitinase was found in flax products as a protein related to defence and stress reactions.
Keywords: flaxseed; cultivar; flour; protein concentrates; proteomic profile; 11S globulin flaxseed; cultivar; flour; protein concentrates; proteomic profile; 11S globulin

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MDPI and ACS Style

Jarošová, M.; Roudnický, P.; Bárta, J.; Zdráhal, Z.; Bártová, V.; Stupková, A.; Lorenc, F.; Bjelková, M.; Kyselka, J.; Jarošová, E.; et al. Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar. Foods 2024, 13, 1288. https://doi.org/10.3390/foods13091288

AMA Style

Jarošová M, Roudnický P, Bárta J, Zdráhal Z, Bártová V, Stupková A, Lorenc F, Bjelková M, Kyselka J, Jarošová E, et al. Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar. Foods. 2024; 13(9):1288. https://doi.org/10.3390/foods13091288

Chicago/Turabian Style

Jarošová, Markéta, Pavel Roudnický, Jan Bárta, Zbyněk Zdráhal, Veronika Bártová, Adéla Stupková, František Lorenc, Marie Bjelková, Jan Kyselka, Eva Jarošová, and et al. 2024. "Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar" Foods 13, no. 9: 1288. https://doi.org/10.3390/foods13091288

APA Style

Jarošová, M., Roudnický, P., Bárta, J., Zdráhal, Z., Bártová, V., Stupková, A., Lorenc, F., Bjelková, M., Kyselka, J., Jarošová, E., Bedrníček, J., & Bohatá, A. (2024). Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar. Foods, 13(9), 1288. https://doi.org/10.3390/foods13091288

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