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Vet. Sci., Volume 12, Issue 9 (September 2025) – 17 articles

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14 pages, 680 KB  
Article
Prevalence and Risk Factors for Salmonella spp. on Pig Carcass, Before and After Chilling, in Brazil
by Anna Carolina Massara Brasileiro, Mariana Avelino de Souza Santos, Cláudia Valéria Gonçalves Cordeiro de Sá, Carla Susana Rodrigues and João Paulo Amaral Haddad
Vet. Sci. 2025, 12(9), 803; https://doi.org/10.3390/vetsci12090803 - 24 Aug 2025
Abstract
The Brazilian Ministry of Agriculture, Livestock and Food Supply conducted a microbiological baseline study to determine the national prevalence of Salmonella in pig carcasses, and characterize the contamination risk according to the abattoir size under federal inspection in 2014–2015. All establishments were size-classified [...] Read more.
The Brazilian Ministry of Agriculture, Livestock and Food Supply conducted a microbiological baseline study to determine the national prevalence of Salmonella in pig carcasses, and characterize the contamination risk according to the abattoir size under federal inspection in 2014–2015. All establishments were size-classified according to their slaughtering capacity per day. Sample weights were defined to increase the external validity of data. Samples were collected after the random selection of two half-carcasses from 76 slaughterhouses, one at the pre-chilling stage and the other at least 12 h after chilling. A total of 1544 samples were analyzed in official laboratories using Vidas SLM and SPT systems; for positive samples, we used the ISO 6579:2014. The results revealed that 10.00% (CI 7.50–13.22) of the pre-chilling carcasses tested positive for Salmonella spp. The medium-sized establishments exhibited the highest prevalence (18.51%). National and international market establishments had prevalences of 17.43%, and 9.05%, respectively. For carcass samples collected after chilling, the estimated prevalence was 4.58% (CI 3.13–6.65), with the highest prevalence recorded in the medium-sized establishments (7%), and values of 12.25% and 3.5% in the NM and IM, respectively. The information and knowledge thus acquired may support the further investigation and evaluation of surveillance programs, improving food safety. Full article
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17 pages, 6170 KB  
Article
Immunogenicity of Virus-like Particles Based on VP1 Protein of Bovine Norovirus
by Zhigang Ma, Xuelian Ma, Xinyu Tao, Yong Huang, Qian Jiang, Xiaojun Ding, Fang Min, Yichen Chu, Ru Li, Xinying Zhang, Lu Liu, Caiyun Zhang, Qi Zhong and Gang Yao
Vet. Sci. 2025, 12(9), 802; https://doi.org/10.3390/vetsci12090802 - 24 Aug 2025
Abstract
Bovine Norovirus (BNoV) is a member of the enterovirus family that can cause gastroenteritis in calves. This virus poses a significant risk to calf growth and development as well as to the long-term sustainability of the cattle industry in China and elsewhere. No [...] Read more.
Bovine Norovirus (BNoV) is a member of the enterovirus family that can cause gastroenteritis in calves. This virus poses a significant risk to calf growth and development as well as to the long-term sustainability of the cattle industry in China and elsewhere. No specific treatment or vaccine is currently available; thus, the development of a safe and effective vaccine is paramount. Here, we describe a strategy to assemble BNoV virus-like particles (VLPs) using the insect baculovirus expression system (BEV) to express the major structural protein, VP1, and demonstrate their potentiality as vaccines. The results showed that the BNoV-VLP self-assembled into complete spherical particles with a diameter of approximately 40 nm. When it was immunized in mice, the levels of specific IgG and IgA antibodies peaked at weeks 6 and 7 post-immunization, respectively, with maximum titers of 1:25,600 and 1:200. Moreover, we observed a significant increase in the CD4+/CD8+ T-cell ratio in splenic lymphocytes of immunized mice (p < 0.05), accompanied by a significant increase in TNF-α+CD4+ T-cells and TNF-α+CD8+ T-cells (p < 0.05). These results demonstrate that BNoV-VLPs are promising vaccine candidates for providing immunoprotection in the future. These studies support the significant practical implications of using a scientific basis for the development of a BNoV-VLP vaccine. Full article
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13 pages, 1447 KB  
Article
Effects of Chromium Yeast Supplementation on Serum hsp60 and hsp70, mRNA Expression in Heat-Stressed Lambs
by Edwin Sandoval-Lozano, Iang S. Rondón Barragán, Andrés Sandoval-Lozano and Román David Castañeda-Serrano
Vet. Sci. 2025, 12(9), 801; https://doi.org/10.3390/vetsci12090801 - 24 Aug 2025
Abstract
Small ruminant production is increasingly affected by heat stress, with recent heat waves highlighting growing economic and welfare-related challenges. Chronic exposure to elevated temperatures disrupts thermoregulation, reduces feed intake, slows growth, compromises meat quality, and increases mortality. This study evaluated the effects of [...] Read more.
Small ruminant production is increasingly affected by heat stress, with recent heat waves highlighting growing economic and welfare-related challenges. Chronic exposure to elevated temperatures disrupts thermoregulation, reduces feed intake, slows growth, compromises meat quality, and increases mortality. This study evaluated the effects of chromium-yeast supplementation at different doses and timepoints on physiological and molecular stress biomarkers in heat-stressed lambs. Forty-eight clinically healthy 6-month-old Katahdin lambs (average weight 20 ± 2.9 kg) were assigned to a 2 × 4 factorial design, with two ambient temperature conditions (heat stress [HS] and thermoneutral [TN]) and four levels of dietary Cr-yeast (0, 0.2, 0.4, and 0.8 mg/kg of dry matter intake). Lambs were housed individually in pens (1.2 × 2.5 m), with ad libitum access to water, and fed a 50:50 corn silage and concentrate diet (excluding mineral premix) twice daily. Blood samples were collected at days 0, 30, and 60 to evaluate plasma cortisol and the expression of hsp60 and hsp70. Chromium bioavailability was assessed by blood levels using absorption chromatography, and glucose clearance was measured at the end of the experiment. Significant reductions in cortisol and hsp70 expression were observed after 30 days of Cr-yeast supplementation under HS conditions (p < 0.05), particularly at the highest dose. For hsp60, a significant reduction was observed at the highest dose on day 30 under HS (p < 0.05). These effects were not sustained on day 60 (p > 0.05). No significant differences were detected under TN conditions (p > 0.05). These findings suggest that Cr-yeast may offer short-term physiological and cellular protection against chronic heat stress in lambs. Full article
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42 pages, 1210 KB  
Review
Comprehensive Prevention and Control of Mastitis in Dairy Cows: From Etiology to Prevention
by Wenjing Yu, Zixuan Zhang, Zhonghua Wang, Xueyan Lin, Xusheng Dong and Qiuling Hou
Vet. Sci. 2025, 12(9), 800; https://doi.org/10.3390/vetsci12090800 - 23 Aug 2025
Abstract
Mastitis, an inflammatory disease caused by the invasion of various pathogenic microorganisms into mammary gland tissue, is a core health issue plaguing the global dairy industry. The consequences of this disease are manifold. In addition to directly compromising the health and welfare of [...] Read more.
Mastitis, an inflammatory disease caused by the invasion of various pathogenic microorganisms into mammary gland tissue, is a core health issue plaguing the global dairy industry. The consequences of this disease are manifold. In addition to directly compromising the health and welfare of dairy cows, it also precipitates a substantial decline in lactation function, a precipitous drop in raw milk production, and alterations in milk composition (e.g., increased somatic cell counts and imbalanced ratios of milk protein to fat). These changes result in a marked degradation of milk quality and safety, and in turn, engender significant economic losses for the livestock industry. Therefore, the establishment and implementation of a comprehensive prevention and control system is a key strategy to effectively curb the occurrence of mastitis, reduce its incidence rate, and minimise economic losses. This review systematically explores the complex etiological factors and pathogenic mechanisms of mastitis in dairy cows, and summarises various diagnostic methods, including milk apparent indicators monitoring, pathogen detection, physiological parameter monitoring, omics technologies, and emerging technologies. Furthermore, it undertakes an analysis of treatment protocols for mastitis in dairy cows, with a particular emphasis on the significance of rational antibiotic use and alternative therapies. Moreover, it delineates preventive measures encompassing both environmental and hygiene management, and dairy cow health management. The objective of this paper is to provide a comprehensive and scientific theoretical basis and practical guidance for dairy farming practices. This will help to improve the health of dairy cows, ensure a stable supply of high-quality dairy products, and promote the sustainable and healthy development of the dairy farming industry. Full article
(This article belongs to the Special Issue Mammary Development and Health: Challenges and Advances)
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15 pages, 618 KB  
Article
Bubulcus ibis, Ciconia ciconia and Erinaceus europaeus from a Wildlife Recovery Center in Portugal as Potential Carriers of Resistant Escherichia coli
by Sofia Santos, Raquel Abreu, Diana Gomes, Catarina Geraldes, Gonçalo Pereira, Isa Serrano, Eva Cunha, Luís Tavares, María Casero and Manuela Oliveira
Vet. Sci. 2025, 12(9), 799; https://doi.org/10.3390/vetsci12090799 - 23 Aug 2025
Abstract
Antimicrobial resistance (AMR) poses an increasing threat to public health, with wildlife recognized as reservoirs and vectors of resistant bacteria. However, the role of wild species in the ecology of AMR remains insufficiently understood, highlighting the need to investigate resistant bacteria in these [...] Read more.
Antimicrobial resistance (AMR) poses an increasing threat to public health, with wildlife recognized as reservoirs and vectors of resistant bacteria. However, the role of wild species in the ecology of AMR remains insufficiently understood, highlighting the need to investigate resistant bacteria in these animals. This study focused on detecting and characterizing Escherichia coli obtained from 43 fecal samples of white storks (Ciconia ciconia), cattle egrets (Bubulcus ibis), and European hedgehogs (Erinaceus europaeus) admitted to a wildlife rehabilitation center in Portugal. Resistance profiles to twelve antibiotics and six virulence factors were characterized phenotypically. ESBL production was also tested. A total of 79 E. coli isolates were obtained from 39 out of 43 samples, and 75 were selected for further characterization. All isolates tested negative for ESBL production. Approximately 64% (n = 48/75) of isolates were resistant to at least one antibiotic, and 5.3% (n = 4/75) were multidrug-resistant. Most frequent resistances were to ampicillin (36%, n = 27/75), tetracycline (12%, n = 9/75), and chloramphenicol (8%), while all isolates were susceptible to meropenem, aztreonam, and third-generation cephalosporins. Most isolates (81.3%, n = 61/75) lacked virulence factors. These findings suggest that wildlife may act as a reservoir of resistant strains, emphasizing importance of AMR monitoring and the One Health approach. Full article
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18 pages, 1289 KB  
Article
Traditional Transportation Methods and Their Influence on Local Chicken Welfare, Behavior, and Blood Profiles: A Policy Considerations
by Saber Y. Adam, Abdelkareem A. Ahmed, Mohammed H. Jammaa, Mohammed Rashid AL Makhmari, Hosameldeen Mohamed Husien, Mohamed Osman Abdalrahem Essa, Hamada Elwan, Mohamed Shehab-El-Deen, Shaaban S. Elnesr, Ahmed A. Saleh and Demin Cai
Vet. Sci. 2025, 12(9), 798; https://doi.org/10.3390/vetsci12090798 - 23 Aug 2025
Abstract
Indigenous chickens are raised in various rural areas in large quantities throughout Sudan. They must be transported over various distances to centralized slaughterhouses or for other purposes. In this study, we examined indigenous chicken farmers’ perceptions of chicken welfare during transportation. A total [...] Read more.
Indigenous chickens are raised in various rural areas in large quantities throughout Sudan. They must be transported over various distances to centralized slaughterhouses or for other purposes. In this study, we examined indigenous chicken farmers’ perceptions of chicken welfare during transportation. A total of 160 indigenous chickens (80 control + 80 transported with their owners) participated in this study. Our findings revealed that 69% and 88% of the farmers indicated that they were not knowledgeable about animal rights and animal welfare, respectively. The majority of the farmers (86%) reported that they were unaware of animal protection laws. Furthermore, the transported chickens showed a significantly long tonic immobility duration (p < 0.05) compared to the control chickens. Moreover, low pecking behavior was significant (p < 0.05) in transported chickens compared to control, particularly on day one of the experiment. In addition, the mean values of glucose, TWBCs, monocytes, basophils, eosinophils, H/L ratio, Hb, MCHC, and PLT were significantly higher (p < 0.05) in transported chickens compared to the controls. In addition, TNF-a, IL-1β, IL-2, IL-4, IFN-γ, IL-17, as well as ROS, MDA, cortisol, glucose, and total cholesterol were significantly higher (p < 0.05) in transportation chickens compared to control, while CAT, GSH, ATP, and SOD were significantly lower (p < 0.05) in transportation chickens compared to control. We conclude that the traditional transportation of indigenous Sudanese chickens affected their welfare, and this was associated with farmers’ low perceptions of chicken welfare, and stress-induced blood profile changes. Full article
13 pages, 394 KB  
Article
Effect of Dietary Supplementation with Salmon Oil on Canine Frozen–Thawed Semen
by Chiara Milani, Marcelo C. Santos, Paolo Zucchini, Barbara Contiero, Stefano Romagnoli, Celia R. Quirino and Isabel C. N. Cunha
Vet. Sci. 2025, 12(9), 797; https://doi.org/10.3390/vetsci12090797 - 23 Aug 2025
Abstract
The objective of this study was to evaluate the effect of omega-3 oral supplementation on frozen–thawed semen quality in stud dogs. Nine healthy male dogs of various ages (2–11 years old), weights (10–36 kg), and breeds were included. Their daily diets were supplemented [...] Read more.
The objective of this study was to evaluate the effect of omega-3 oral supplementation on frozen–thawed semen quality in stud dogs. Nine healthy male dogs of various ages (2–11 years old), weights (10–36 kg), and breeds were included. Their daily diets were supplemented with salmon oil at 180 mg DHA/7kg BW. Semen was collected before salmon oil oral administration (D0), after 90 days of daily administration (D90), and 90 days following suspension (D180). Samples underwent standard freezing procedures and were stored for at least 30 days; then, they were thawed at 37°C for 1 min. Samples (n = 27) were assessed post-thawing for motility (MOT%), membrane integrity (HOST%), and viability (EN%). A second aliquot of the same samples underwent a thermoresistance test at 37°C, with MOT% and EN% evaluated at 2 and 4 h post-thawing. Data were analyzed using repeated measures ANOVA, considering days of administration (D0, D90, D180), incubation times at 37°C (T0h-T2h-T4h) and their interaction. Significance was set at P < 0.05. An increase in MOT% was observed after 90 days of fish oil administration (D0 = 56.67 ± 5.1% vs. D90 = 71.67 ± 5.1%, p < 0.05); however, this effect was not maintained at D180 (45.56 ± 5.1%). Similar results were observed for HOST and EN. During the thermoresistance test, a significantly higher MOT% was observed at T4h on D90 compared to D0 (p < 0.05). These findings indicate that daily salmon oil supplementation at 180 mg DHA/7 kg for 90 days improves semen motility, membrane integrity and viability, supporting a role for omega-3 fatty acids in semen undergoing cryopreservation. Full article
(This article belongs to the Section Veterinary Reproduction and Obstetrics)
10 pages, 452 KB  
Article
Retrospective Evaluation of Cryptorchid Sidedness at Colorado State University Between 1984 and 2014 and Oakridge Equine Hospital Between 2008 and 2023
by Hannah Fain, Dean A. Hendrickson, Matthew T. Buesing and Gregg Griffenhagen
Vet. Sci. 2025, 12(9), 796; https://doi.org/10.3390/vetsci12090796 - 23 Aug 2025
Abstract
Cryptorchidism is a common congenital disorder in male horses characterized by the failure of one or both testes to descend into the scrotum. This developmental anomaly has important clinical, surgical, and economic implications, particularly in breeding animals. This retrospective study investigates the prevalence [...] Read more.
Cryptorchidism is a common congenital disorder in male horses characterized by the failure of one or both testes to descend into the scrotum. This developmental anomaly has important clinical, surgical, and economic implications, particularly in breeding animals. This retrospective study investigates the prevalence and laterality of cryptorchidism in a large equine population presented to two veterinary referral hospitals—Colorado State University Veterinary Teaching Hospital (CSU VTH, 1984–2014) and Oakridge Equine Hospital (OEH, 2008–2023). Medical records were reviewed to identify affected horses, and data on breed, age, and laterality of retained testes were collected. Anatomical location of retention (inguinal vs. abdominal) was excluded due to inconsistent documentation across the study period. A total of 777 horses met the inclusion criteria, with Quarter Horses comprising the majority of clinical cases. Quarter Horses exhibited a strong predisposition for left-sided testicular retention, whereas Thoroughbreds and Arabians more commonly presented with right-sided retention. These breed-specific trends in laterality suggest possible developmental factors influencing testicular descent. Understanding these patterns can aid in clinical diagnosis, improve surgical planning, and contribute to evidence-based breeding recommendations aimed at reducing the incidence of cryptorchidism in equine populations. Full article
(This article belongs to the Section Veterinary Surgery)
24 pages, 633 KB  
Review
Zoonotic Yersinia enterocolitica in Swine: One Health Implications for Food Safety and Public Health
by Sónia Saraiva, Juan García-Díez, Telma de Sousa, Rita Calouro, Diana Fernandes, Ana V. Mourão, Cristina Saraiva, João R. Mesquita, Ana C. Coelho and Patrícia Poeta
Vet. Sci. 2025, 12(9), 795; https://doi.org/10.3390/vetsci12090795 - 23 Aug 2025
Abstract
Y. enterocolitica is a cold-tolerant, foodborne pathogen that poses a significant risk to public health, with pigs identified as its main reservoir. This review explores the current knowledge on the epidemiology, transmission, and virulence of Y. enterocolitica in pigs, highlighting their central role [...] Read more.
Y. enterocolitica is a cold-tolerant, foodborne pathogen that poses a significant risk to public health, with pigs identified as its main reservoir. This review explores the current knowledge on the epidemiology, transmission, and virulence of Y. enterocolitica in pigs, highlighting their central role in spreading the bacterium to humans—primarily through the consumption of raw or undercooked pork. The pathogen is frequently found in pig tonsils and intestines and can contaminate meat during slaughter. Among the various strains, bioserotype 4/O:3 is the most common cause of human illness. Y. enterocolitica carries a diverse set of virulence genes, such as ail, yst, inv, and yad, and evades immune responses. The review also covers major outbreaks, risk factors on farms and in slaughterhouses, and the limitations of current surveillance systems. Reducing the impact of Y. enterocolitica requires a One Health approach linking animal health, food safety, and public health. Full article
(This article belongs to the Special Issue Emerging Bacterial Pathogens in Veterinary Medicine)
23 pages, 1114 KB  
Review
Dietary Supplementation of Methionine, Tryptophan, and Threonine for Pigs Under Sanitary Challenges: Current Knowledge and Future Directions
by Graziela da Cunha Valini, Alícia Zem Fraga, Ismael França, Danilo Alves Marçal, Pedro Righetti Arnaut, Alini Mari Veira, Marllon José Karpeggiane de Oliveira, Ines Andretta and Luciano Hauschild
Vet. Sci. 2025, 12(9), 794; https://doi.org/10.3390/vetsci12090794 - 23 Aug 2025
Abstract
Sanitary challenges (SCs) may alter the health status, growth performance, and pigs’ welfare. Changes in amino acid (AA) plasma concentrations have been observed in inflammatory-challenged pigs which may be associated with key factors, such as: (1) the synthesis of immune components to support [...] Read more.
Sanitary challenges (SCs) may alter the health status, growth performance, and pigs’ welfare. Changes in amino acid (AA) plasma concentrations have been observed in inflammatory-challenged pigs which may be associated with key factors, such as: (1) the synthesis of immune components to support innate and/or adaptive immune responses, (2) the redistribution of nutrients from growth and production functions toward cells and tissues involved in inflammatory and immune responses, and (3) decreased anabolism and/or increased catabolism of skeletal muscle to increase the availability of nutrients, often as a consequence of reduced feed intake. Due to their health-promoting effects, nutritional strategies involving AA may help mitigate the negative impacts of SC. Methionine, tryptophan, and threonine, beyond serving as protein building blocks, are considered functional AA because they support immune system function, enhance intestinal barrier integrity, modulate inflammatory responses, and limit oxidative stress. Additionally, the review highlights the influence of individual variability, such as differences in body weight, on nutritional requirements and responses to AA supplementation for pigs under SC. The integration of nutritional strategies tailored to immune-challenged pigs offers promising avenues to improve productivity and animal welfare in commercial swine production systems with increasing restrictions on antibiotic use. Full article
(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)
20 pages, 4055 KB  
Article
Antimicrobial Resistance and Genomic Characterization of an Escherichia coli Strain Harboring p0111 and an IncX1-Type Plasmid, Isolated from the Brain of an Ostrich
by Jing Hu, Jiahe Zhou, Leping Wang, Zhongwei Chen, Yizhou Tan, Yangyan Yin, Zhe Pei, Changting Li, Huili Bai, Chunxia Ma, Ling Teng, Yongcui Feng, Xian Li, Yingyi Wei and Hao Peng
Vet. Sci. 2025, 12(9), 793; https://doi.org/10.3390/vetsci12090793 - 22 Aug 2025
Abstract
An outbreak characterized by clinical signs of diarrhea and paralysis, occasionally progressing to fatal outcomes, occurred at an ostrich breeding facility. Conventional antibiotic treatments proved ineffective. To investigate the etiology of the disease, brain and liver specimens were collected for diagnostic analysis. An [...] Read more.
An outbreak characterized by clinical signs of diarrhea and paralysis, occasionally progressing to fatal outcomes, occurred at an ostrich breeding facility. Conventional antibiotic treatments proved ineffective. To investigate the etiology of the disease, brain and liver specimens were collected for diagnostic analysis. An Escherichia coli (E. coli) isolate, designated strain HZDC01, was obtained from cerebral tissues, and whole-genome sequencing was performed for genomic characterization. Genomic analysis revealed that the chromosomal DNA harbors numerous resistance genes, conferring multidrug resistance through complex mechanisms. Furthermore, a p0111-type plasmid carrying the blaCTX-M-55 gene and an IncX1-type plasmid harboring rmtB, sul1, APH(6)-Id, tet(A), AAC(3)-IIc, aadA2, blaTEM-1B, and floR genes were identified. These plasmids carry numerous mobile genetic elements that can disseminate via horizontal gene transfer, thereby amplifying the risk of resistance-gene spread within bacterial populations. Additionally, the ibeB and ibeC genes, which encode proteins involved in the invasion of brain microvascular endothelial cells, were identified. These genes may facilitate E. coli penetration of the blood–brain barrier, potentially leading to meningitis and posing a life-threatening risk to the host. This is the first report of the isolation and characterization of extended-spectrum beta-lactamase E. coli from the brain of an ostrich with paralysis. The findings provide valuable genomic insights into the antimicrobial resistance profiles and pathogenic mechanisms of ostrich-derived E. coli isolates. Full article
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15 pages, 2319 KB  
Article
Evaluation of the Interest in and Tolerance of a Topical Emollient in the Management of Canine Nasal Hyperkeratosis: An Open-Label, Prospective, Uncontrolled Pilot Study
by Sébastien Viaud, Sarah Pariente, Bruno Jahier, Christelle Navarro, Cécile Destaing and Carole Gard
Vet. Sci. 2025, 12(9), 792; https://doi.org/10.3390/vetsci12090792 - 22 Aug 2025
Abstract
(1) Background: Idiopathic or familial nasal hyperkeratosis (NHK) may be considered a cosmetic issue in its uncomplicated form. Nevertheless, the prevention of secondary lesions could be advised by proper management. (2) Methods: In this multicentre, prospective, open-label pilot study, dogs with familial or [...] Read more.
(1) Background: Idiopathic or familial nasal hyperkeratosis (NHK) may be considered a cosmetic issue in its uncomplicated form. Nevertheless, the prevention of secondary lesions could be advised by proper management. (2) Methods: In this multicentre, prospective, open-label pilot study, dogs with familial or idiopathic NHK received a topical emollient balm twice daily for 60 days. Evaluation of the global dermatological score (GDS; 0–12) on D0, D30, and D60 by owners and investigators was the main outcome. The score’s percentage decrease versus D0; the evolution of “dryness”, “lichenification”, “crusts”, and “affected area” (0–3); the correlation between observers and the evaluation of clinical improvement; and animal discomfort rated using a VAS (0–10) during follow-up were analysed. (3) Results: Twenty dogs completed the study. The mean GDS decreased significantly from Day 0 to Day 30 and Day 60 (p < 0.0001), with percentage reductions of 44.9% and 54.5% (investigators) and 54.3% and 62.3% (owners) on Day 30 and Day 60, respectively. Correlations between investigators and owners were significant for dryness, crusts (at Day 60), and the affected area, but not for lichenification. Animal discomfort scores decreased by more than 50% from Day 0. Owners reported high satisfaction with the product (mean score 8.1/10) and no adverse events occurred. (4) Conclusions: This open-label pilot study suggests that the tested balm is well tolerated and may improve clinical signs of NHK in dogs. Given the lack of a control group, small sample size, and absence of blinding, these results should be considered preliminary. Further randomized controlled trials and formal validation of the scoring system are warranted. Full article
17 pages, 1289 KB  
Article
Live Yeast Supplementation Attenuates the Effects of Heat Stress in Dairy Cows
by Ana R. J. Cabrita, Júlio Carvalheira and António J. M. Fonseca
Vet. Sci. 2025, 12(9), 791; https://doi.org/10.3390/vetsci12090791 - 22 Aug 2025
Abstract
High temperature typically decreases feed intake, milk production, and efficiency and increases metabolic disorders and health problems, greatly impacting farm economics. Supplements based on Saccharomyces cerevisiae have been suggested to benefit cows under heat stress, but effects on dairy cow performance are contradictory. [...] Read more.
High temperature typically decreases feed intake, milk production, and efficiency and increases metabolic disorders and health problems, greatly impacting farm economics. Supplements based on Saccharomyces cerevisiae have been suggested to benefit cows under heat stress, but effects on dairy cow performance are contradictory. This study aimed to evaluate the influence of heat stress on the effects of live yeast supplementation on the performance of dairy cows. Environmental temperature parameters were compared to two thermal humidity indices (THI1 and THI2) using wet bulb or dew point temperatures, as explanatory variables of dairy cow performance during the hot season. The experiment followed a randomized complete block design with 12 Holstein cows blocked by lactation number, days in milk, and milk production (two cows per block) and within each block, each cow was randomly assigned to a maize silage-based TMR with a concentrate mixture containing no yeast culture (Control) or 1 g/kg concentrate dry matter of a live yeast culture based on S. cerevisiae (Yeast) for 35 days. The experiment lasted for 35 d. Dry matter intake (DMI) was significantly higher for Yeast than it was for Control for all classes of temperature and THIs studied with an average increase of 2 kg DM per day, except for mean THI1 (from 54 to 60), for which the DMI was similar between treatments. Yeast promoted significantly higher milk yield than Control for all classes of daily maximum and mean temperature, averaging an increase of 4 kg of milk per day. Results suggest a more marked effect of temperature and indicate that yeast supplementation improved lactation performance of dairy cows exposed to hot weather. Full article
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16 pages, 464 KB  
Article
Physiological and Metabolic Responses to Water Restriction in Ewes Under Semi-Arid Conditions
by Claudenilde de Jesus Pinheiro Costa, Gherman Garcia Leal de Araújo, André Luiz Rodrigues Magalhães, Alberício Pereira de Andrade, Silvia Helena Nogueira Turco, Maria Helena Tavares de Matos, Diego César Nunes da Silva, Cleyton de Almeida Araújo, Roberta de Lima Valença, Thieres George Freire da Silva, Fleming Sena Campos and Glayciane Costa Gois
Vet. Sci. 2025, 12(9), 790; https://doi.org/10.3390/vetsci12090790 - 22 Aug 2025
Abstract
The aim of this study was to evaluate the effect of different water restrictions on the thermoregulation and blood hematological and metabolite parameters of crossbred Santa Inês ewes in a semi-arid climate. Thirty-two ewes were subjected to four water supply levels (100%, 80%, [...] Read more.
The aim of this study was to evaluate the effect of different water restrictions on the thermoregulation and blood hematological and metabolite parameters of crossbred Santa Inês ewes in a semi-arid climate. Thirty-two ewes were subjected to four water supply levels (100%, 80%, 60%, and 40%), in a completely randomized design with eight replications. The confinement period lasted 77 days, with 14 days allocated for adaptation. Respiratory rate, heart rate, and rectal temperature exhibited a quadratic response. There was an increase in red blood cells and urea. The enzyme alanine aminotransferase decreased linearly with water restriction. Urinary creatinine decreased along with water supply. Regarding urine color characteristics, all groups showed different colors, ranging from clear to cloudy. For the chemical characteristics of urine, a quadratic effect was observed for pH, with the highest value (8.75) at 60%. An increase was observed in total urine proteins and urobilinogen. Crossbred Santa Inês ewes in a semi-arid climate exhibit physiological adaptations to water supply reduction up to 40%. Following an 80% reduction in water supply, animals exhibit mild dehydration, characterized by increased serum urea levels and decreased alanine aminotransferase activity. Full article
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15 pages, 1771 KB  
Article
Inhibition of NOX4-Mediated ROS Production Contributes to Selenomethionine’s Anti-Inflammatory Effect in LPS-Stimulated Bovine Endometrial Epithelial Cells
by Luying Cui, Wanting Li, Sasa He, Long Guo, Kangjun Liu, Junsheng Dong, Jianji Li and Heng Wang
Vet. Sci. 2025, 12(9), 789; https://doi.org/10.3390/vetsci12090789 - 22 Aug 2025
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Abstract
Bovine endometritis can be caused by Escherichia coli (E. coli), from which the lipopolysaccharide (LPS) triggers TLR4/NF-κB-mediated inflammation and reactive oxygen species (ROS) overproduction, resulting in impaired reproductive performance. While NADPH oxidase (NOX) is a critical source of ROS generation, its [...] Read more.
Bovine endometritis can be caused by Escherichia coli (E. coli), from which the lipopolysaccharide (LPS) triggers TLR4/NF-κB-mediated inflammation and reactive oxygen species (ROS) overproduction, resulting in impaired reproductive performance. While NADPH oxidase (NOX) is a critical source of ROS generation, its role in bovine endometrial epithelial cells (BEEC) and modulation by selenium remains unexplored. In this study, primary BEEC was challenged by LPS to assess NOX2/4 expression kinetics. Inhibitors of NOX and NF-κB were applied to observe the role of NOX-derived ROS in BEEC inflammation and in selenomethionine (SeMet)-modulated anti-inflammation. ROS levels were measured by flow cytometry. The changes in inflammatory cytokines, and the proteins related to NOX4 and NF-κB, were analyzed via qPCR and Western blot. As a result, the inhibition of NOX decreased LPS-induced proinflammatory cytokine expression, ROS accumulation, NOX4 level, and the phosphorylation of NF-κB P65 and IκBα. Conversely, the suppression of NF-κB downregulated the levels of ROS and NOX4. Cotreatment with SeMet and a NOX inhibitor further suppressed the inflammatory response, ROS level, and NF-κB pathway activation compared to individual treatment, but had no additive effect on the NOX4 protein level. In conclusion, the NOX4/ROS/NF-κB axis forms a proinflammatory feedback loop in LPS-stimulated BEEC. SeMet mitigates oxidative stress and inflammation partially through NOX4 inhibition. Full article
(This article belongs to the Special Issue Prevention and Control of Obstetric Diseases in Domestic Animals)
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9 pages, 208 KB  
Article
Incidence of Bacterial Colonization of Intravenous Non-Permanent Venous Catheters in Hospitalized Equine Patients
by Valentina Vitale, Francesca Bindi, Fabrizio Bertelloni, Giulia Sala, Dania Cingottini, Francesca Bonelli and Micaela Sgorbini
Vet. Sci. 2025, 12(9), 788; https://doi.org/10.3390/vetsci12090788 - 22 Aug 2025
Viewed by 43
Abstract
Bacterial contamination of intravenous catheters in hospitalized horses may pose risks for both patient care and public health due to potential antimicrobial resistance and zoonotic transmission. This prospective clinical study aimed to evaluate the incidence of catheter contamination in equine patients admitted to [...] Read more.
Bacterial contamination of intravenous catheters in hospitalized horses may pose risks for both patient care and public health due to potential antimicrobial resistance and zoonotic transmission. This prospective clinical study aimed to evaluate the incidence of catheter contamination in equine patients admitted to a Veterinary Teaching Hospital between 2020 and 2021. All horses requiring intravenous catheterization were included, and data were collected on patient signalment, clinical status, duration of catheterization, treatments, and outcomes. Two catheter types were used: 5 cm polytetrafluoroethylene and 11 cm polyurethane, both 14-gauge. Catheters were aseptically removed, and the distal ends were cultured on Blood Agar and Brain Heart Infusion broth. Bacterial isolates were identified using standard microbiological techniques, and contamination was defined as any detectable bacterial growth. Of the 58 catheters collected from 52 patients, 38 (65.5%) showed bacterial growth, predominantly Staphylococcus spp. Multivariable logistic regression analysis identified antibiotic treatment as significantly associated with positive bacteriological culture. These findings indicate a higher contamination rate than previously reported in horses. While colonization may not always result in clinical infection, the observed frequency of contamination underscores the need for careful catheter management and further investigation into its implications for antimicrobial stewardship. Full article
(This article belongs to the Section Veterinary Internal Medicine)
16 pages, 6764 KB  
Article
Hepatocellular Early Apoptosis Associated with HES 130/0.4 Administration for Volume Replacement in Pigs After Severe Bleeding
by Helena Vala, Ana I. Faustino-Rocha, Rita Cruz, Carlos Venâncio, Aura Silva, João R. Mesquita, Ana Liza Ortiz and David A. Ferreira
Vet. Sci. 2025, 12(9), 787; https://doi.org/10.3390/vetsci12090787 - 22 Aug 2025
Viewed by 258
Abstract
Hydroxyethyl starch (HES) 130/0.4 is commonly used for volume replacement, yet its hepatic effects in the context of acute haemorrhage remain unclear. This study aimed to evaluate hepatic histopathological changes related to HES 130/0.4 administration when compared to Ringer’s lactate (RL) in healthy [...] Read more.
Hydroxyethyl starch (HES) 130/0.4 is commonly used for volume replacement, yet its hepatic effects in the context of acute haemorrhage remain unclear. This study aimed to evaluate hepatic histopathological changes related to HES 130/0.4 administration when compared to Ringer’s lactate (RL) in healthy pigs subjected to acute bleeding under general anaesthesia. Eighteen pigs were randomised into three groups: RL (n = 6), HES 130/0.4 (n = 6), and a non-bleeding control (n = 6). Liver tissue was collected postmortem and analysed using haematoxylin–eosin staining, cytochrome c immunohistochemistry, the TUNEL assay, and M30 immunofluorescence. No statistically significant differences were observed in general histopathological changes, TUNEL, or cytochrome c expression (p > 0.050). However, the pigs that received HES 130/0.4 for volume replacement showed significantly higher intensity of the liver M30 immunostaining in the Q-score (p < 0.010), H-score (p < 0.010), and c indexc index (p < 0.050) when compared to animals that received Ringer’s lactate solution or animals in the control group. These findings suggest that HES 130/0.4 induces increased early hepatocellular apoptosis when compared to RL in this model, raising concerns about its hepatic safety profile under haemorrhagic conditions. Full article
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