Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth
by
, , and
Xun Fang
1, 
Bereket Molla Tanga
1
,
Seonggyu Bang
1,
Chaerim Seo
1,
Heyyoung Kim
2,
Islam M. Saadeldin
1,3
,
Sanghoon Lee
1 and
Jongki Cho
1,*
1
Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea
2
School of Biological Sciences and Technology, College of Natural Sciences, Chonnam National University, Gwangju 34134, Korea
3
Research Institute of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea
*
Author to whom correspondence should be addressed.
Vet. Sci. 2022, 9(11), 609; https://doi.org/10.3390/vetsci9110609
Submission received: 21 September 2022
/
Revised: 20 October 2022
/
Accepted: 29 October 2022
/
Published: 4 November 2022
(This article belongs to the Special Issue New Insights in Veterinary Theriogenology)
Simple Summary
We investigated the effects of extracellular vesicles derived from the oviduct on porcine blastocyst attachment as a model for preimplantation embryo implantation. Embryo developmental competence and pluripotency gene expression were highly expressed after extracellular vesicles supplementation.
Abstract
Porcine species have a great impact on studies on biomaterial production, organ transplantation and the development of biomedical models. The low efficiency of in vitro-produced embryos to derive embryonic stem cells has made achieving this goal a challenge. The fallopian tube plays an important role in the development of embryos. Extracellular vesicles (EVs) secreted by oviductal epithelial cells play an important role in the epigenetic regulation of embryo development. We used artificially isolated oviductal epithelial cells and EVs. In this study, oviductal epithelial cell (OEC) EVs were isolated and characterized through transmission electron microscopy, nanoparticles tracking analysis, western blotting and proteomics. We found that embryo development and blastocyst formation rate was significantly increased (14.3% ± 0.6% vs. 6.0% ± 0.6%) after OEC EVs treatment. According to our data, the inner cell mass (ICM)/trophectoderm (TE) ratio of the embryonic cell number increased significantly after OEC EVs treatment (43.7% ± 2.3% vs. 28.4% ± 2.1%). Meanwhile, the attachment ability of embryos treated with OEV EVs was significantly improved (43.5% ± 2.1% vs. 29.2% ± 2.5%, respectively). Using quantitative polymerase chain reaction (qPCR), we found that the expression of reprogramming genes (POU5F1, SOX2, NANOG, KLF4 and c-Myc) and implantation-related genes (VIM, KRT8, TEAD4 and CDX2) significantly increased in OEC EV-treated embryos. We report that OEC EV treatment can improve the development and implantation abilities of embryos.
