Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring
Abstract
:1. Introduction
2. Methods
2.1. Strains
2.2. Germination Conditions
2.3. Video Microscopy
2.4. Nucleus and Septum Staining
2.5. Transcriptome
2.6. Epigenetic Inhibitors
2.7. Statistical Analysis
3. Results
3.1. Characterization of Conidial Germination in A. fumigatus
3.2. Does the Nutritional Environment Modify the Conidial Germination Asynchrony?
3.3. Nuclear Division and Septum Formation in Different Nutritive Environments
3.4. Growth Arrest in G, GA and GAP Media Is Caused by Exhaustion of Nutrients Present in the Conidia
3.5. Transcriptome Analysis
3.6. Trying to Modify the Asynchrony of Conidial Germination in A.fumigatus
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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G | GA | GAP | MM | |
---|---|---|---|---|
Conidia area at T0, mean, px | 263 ± 9 a | 272 ± 13 a | 253 ± 21 a | 254 ± 17 a |
Conidia area at 6 h, px | 355 ± 10 b | 378 ± 13 b | 416 ± 21 a | 519 ± 17 a |
Conidia area at 8 h, px | 395 ± 10 c | 412 ± 13 c | 518 ± 13 b | 757 ± 21 a |
Conidia area at 16 h, px | 471 ± 10 c | 460 ± 16 b | 1078 ± 24 a | NA |
Conidia area at first germ tubes appearance, px | 620 ± 52 c | 545 ± 29 c | 1435 ± 39 a | 1184 ± 16 b |
Time at first germ tube appearance, min | 1223 ± 51 a | 834 ± 28 b | 843 ± 38 b | 528 ± 15 c |
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Danion, F.; van Rhijn, N.; Dufour, A.C.; Legendre, R.; Sismeiro, O.; Varet, H.; Olivo-Marin, J.-C.; Mouyna, I.; Chamilos, G.; Bromley, M.; et al. Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring. J. Fungi 2021, 7, 30. https://doi.org/10.3390/jof7010030
Danion F, van Rhijn N, Dufour AC, Legendre R, Sismeiro O, Varet H, Olivo-Marin J-C, Mouyna I, Chamilos G, Bromley M, et al. Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring. Journal of Fungi. 2021; 7(1):30. https://doi.org/10.3390/jof7010030
Chicago/Turabian StyleDanion, François, Norman van Rhijn, Alexandre C. Dufour, Rachel Legendre, Odile Sismeiro, Hugo Varet, Jean-Christophe Olivo-Marin, Isabelle Mouyna, Georgios Chamilos, Michael Bromley, and et al. 2021. "Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring" Journal of Fungi 7, no. 1: 30. https://doi.org/10.3390/jof7010030