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Agronomy
Special Issues
Plant Tissue Culture and Plant Somatic Embryogenesis–2nd Edition
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Plant Tissue Culture and Plant Somatic Embryogenesis–2nd Edition

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Plant-Crop Biology and Biochemistry".

Deadline for manuscript submissions: 31 October 2024 | Viewed by 742

Special Issue Editors

Dr. Justyna Lema-Rumińska

E-Mail Website
Guest Editor
Department of Environmental Biology, Faculty of Biological Science, Kazimierz Wielki University,12 Ossoliński Av., PL-85-093 Bydgoszcz, Poland
Interests: somatic embryogenesis; secondary metabolites; molecular markers
Special Issues, Collections and Topics in MDPI journals
Dr. Danuta Kulpa

E-Mail Website
Guest Editor
Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, 17 Słowackiego Str., PL-71-434 Szczecin, Poland
Interests: micropropagation; secondary metabolites; suspension cultures
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant in vitro cultures and plant somatic embryogenesis is the focus of this 2nd Edition of the Special Issue entitled ‘Plant Tissue Culture and Plant Somatic Embryogenesis’. The methods employed to propagate and regenerate plants in in vitro cultures are being developed and enhanced continuously. Micropropagation is extensively used for the production of high-quality cuttings, and it is also employed in gene banks for the in vitro conservation and storage of plant genetic resources. A micropropagation is also a crucial tool for the production of specific secondary metabolites of medicinal plants. One of the most efficient plant regeneration methods is somatic embryogenesis. However, the genetic stability of plants can be disrupted during micropropagation and regeneration processes. Therefore, ensuring the true-to-type nature of plants obtained through these methods requires confirmation of their genetic stability. Molecular markers, applicable at any stage of plant development, are particularly effective for this purpose. The genetic variability induced through techniques such as mutagenesis, or genetic transformation, in in vitro cultures also facilitates the breeding of novel crop cultivars.

Considering these advancements, researchers are encouraged to publish original research and review articles presenting methods that are applicable to plant propagation and regeneration in vitro cultures, particularly focusing on somatic embryogenesis. Manuscripts addressing the determination of the genetic stability of plants post-regeneration, micropropagation, or the use of in vitro culture methods for secondary metabolite production or plant breeding are highly welcome.

Dr. Justyna Lema-Rumińska
Dr. Danuta Kulpa
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • breeding
  • elicitation
  • genetic stability
  • micropropagation
  • molecular markers
  • regeneration
  • slow growth
  • somatic embryos

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Published Papers (1 paper)

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Research

20 pages, 8691 KiB  
Article
Comparative Transcriptomic Insights into the Mechanisms Underlying Maize (Zea mays L.) Embryogenic Callus Differentiation
by Liqiang Dai and Tianjiao Li
Agronomy 2024, 14(8), 1689; https://doi.org/10.3390/agronomy14081689 - 31 Jul 2024
Viewed by 507
Abstract
The regeneration of plant somatic cells is a prerequisite for their biological breeding. Identification of key genes controlling embryogenic callus (EC) differentiation and investigation of the genetic mechanism of cell fate determination are important for improving plant variety. In this study, we used [...] Read more.
The regeneration of plant somatic cells is a prerequisite for their biological breeding. Identification of key genes controlling embryogenic callus (EC) differentiation and investigation of the genetic mechanism of cell fate determination are important for improving plant variety. In this study, we used the maize inbred line KN5585 and its gene-edited mutants Zmprx19-1, Zmprx19-2 and Zmprx19-3 as plant materials. Three somatic regeneration-related traits, the embryogenic callus induction rate (EIR), green callus rate (GCR) and plantlet regeneration rate (PRR), were identified by tissue culture of immature embryos. Additionally, the ECs at different differentiation stages (0 d, 5 d, 10 d and 15 d) were subjected to RNA-seq, and comparative transcriptome analyses were performed. The results showed that the somatic regeneration traits of the mutants were all highly significantly lower than those of the wild type (p < 0.01). The PRR value of KN5585 was 75.25%, while the highest PRR of the mutants was only 15.08%, indicating that knockdown of ZmPRX19 inhibited EC regeneration. Transcriptome sequencing yielded a total of 200.30 Gb of clean data from 24 libraries, with an average of 6.53 Gb of clean data per library. Mutant and wild-type gene expression data were compared separately at four differentiation stages, and 689 common differentially expressed genes (DEGs) were screened. WGCNA was used to classify these genes into nine modules, which were subsequently subjected to GO and KEGG enrichment analyses. In total, 40, 23, 17 and 5 genes were significantly (q < 0.05) enriched in plant hormone signal transduction, the MAPK signaling pathway-plant, phenylpropanoid biosynthesis and photosynthesis, respectively. Moreover, protein–protein interaction (PPI) network analysis revealed five MAPKKK17_18 hub nodes involved in the MAPK pathway-plant, which may be the key genes controlling plantlet differentiation from ECs. The above results provide a basis for the final elucidation of the molecular mechanism of plant somatic regeneration. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Plant Somatic Embryogenesis–2nd Edition)
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