Meat Quality and Protein Expression in Livestock and Poultry (Volume II)

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Nutrition".

Deadline for manuscript submissions: closed (15 September 2023) | Viewed by 4539

Special Issue Editors


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Guest Editor
Department of Animal Genetics, Breeding and Ethology, Faculty of Animal Science, University of Agriculture in Krakow, Krakow, Poland
Interests: meat quality; muscle structure; ovary; ovarian follicles; cell biology; histology; histopathology; immunohistochemistry; protein expression; apoptosis; oxidative stress
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Guest Editor
Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland
Interests: avian physiology; embryogenesis of birds; mRNA expression and immunodetection of apoptosis and oxidative stress regulators; toxicology

Special Issue Information

Dear Colleagues,

Muscle is a major source of protein for humans. Currently, as food supplies are more plentiful, meat quality is becoming increasingly important to consumers. Today, we know that meat quality is the result of many factors, such as animal nutrition, rearing and slaughter, and genotype. The long-term intensive selection of livestock and poultry, with the aim of improving weight gain and dressing percentage in increasingly short periods of time, has contributed to undesirable metabolic and physiological disorders in muscle tissue microstructures, which have adversely affected meat quality. Theories regarding the problem of increasing meatiness and decreasing meat quality in livestock and poultry often use research into muscle tissue microstructures and genetics at the molecular level. It is exactly these muscle microstructure parameters, in particular, muscle fiber composition, intramuscular fat content, and intramuscular connective tissue, which determine the physico-chemical parameters of meat (such as acidity, color, tenderness, drip loss, and water-holding capacity) and thus its quality. It is generally accepted that during the conversion of muscle to meat, postmortem changes that occur in the muscle tissue and determine the subsequent production and culinary usefulness of the meat are dependent, among other things, on the rate of myofibrillar and cytoskeletal protein degradation. In the last decade, proteomic analyses have shown that protein degradation is associated with energy metabolism, cellular death cascades (apoptosis or autophagy), cellular survival (heat shock and chaperone proteins), and oxidative stress. The elucidation of these processes and their impacts on postmortem muscle proteins, as well as their inherent interactions, is key to understanding the mechanisms that influence meat quality.

This second volume of the Special Issue welcomes both review and research papers regarding all aspects of the relationships between meat structure, protein expression, and meat quality. Areas of interest include the effect of breed, line, gender, nutrition, aging time, muscle type, etc., on meat quality and its structure and rate of protein degradation. Topics of special interest are the muscle development and growth mechanisms of livestock and poultry in relation to muscle myopathies and meat quality.

I invite you to share your recent findings in this Special Issue.

Prof. Dr. Dorota Wojtysiak
Dr. Agnieszka Grzegorzewska
Guest Editors

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Keywords

  • meat quality

  • microstructure
  • muscle type
  • skeletal muscle proteins
  • postmortem aging
  • muscle myopathies
  • apoptosis
  • oxidative stress
  • heat shock proteins
  • breed
  • nutrition

Published Papers (3 papers)

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Research

13 pages, 2549 KiB  
Article
Effect of Spirulina platensis Supplementation on Carcass Characteristics, Fatty Acid Profile, and Meat Quality of Omani Goats
by Fahad Al-Yahyaey, Waleed Al-Marzooqi, Ihab Shaat, Melanie A. Smith, Jamal Al-Sabahi, Sherif Melak and Russell D. Bush
Animals 2023, 13(18), 2976; https://doi.org/10.3390/ani13182976 - 20 Sep 2023
Cited by 2 | Viewed by 1350
Abstract
In a 70-day study, 36 Jabbali and Sahrawi bucks, aged 11 months, were utilized to evaluate the effects of different levels of spirulina dietary supplement (SP) on carcass characteristics, fatty acid profile, and meat quality traits in Omani goat breeds. The goats were [...] Read more.
In a 70-day study, 36 Jabbali and Sahrawi bucks, aged 11 months, were utilized to evaluate the effects of different levels of spirulina dietary supplement (SP) on carcass characteristics, fatty acid profile, and meat quality traits in Omani goat breeds. The goats were put into six groups of six bucks, each at random. The diet consisted of a conventional concentrate feed ration (CFR) without spirulina (CON), and the CFR diet supplemented with spirulina at the levels of 2 g/head daily (T1) and 4 g/head daily (T2). In general, Sahrawi bucks showed a highly significant response to SP feeding compared with Jabbali bucks. The treatment groups, especially T1, showed a significant increase in average daily gain and carcass traits (body length, leg length, and the rack weight) compared with the CON group of Sahrawi bucks. The weights of omental and kidney fat were also significantly higher in T1 compared with CON and T2 groups of Sahrawi goats, while they were significantly higher in T2 compared with CON and T1 groups of Jabbali goats. Carcass profile and meat quality, including ultimate ph and meat color lightness (L*) were increased significantly with dietary spirulina in both LD and SM muscles of Sahrawi goats. Most of the Sfa, Mufa, Pufa, Pufa n-6, Pufa n-3, and n-6/n-3 ratios of the LD showed significant differences in diets supplemented with SP compared with CON for Sahrawi bucks, while some of them were significant in Jabbali bucks. The LD muscle of Sahrawi goats fed diets supplemented with SP of the T1 group significantly decreased in the amounts of pentadecanoic and margaric acids compared with the T2 and CON groups. The study concluded that incorporating SP (2 g and 4 g/head daily) into the diet of Omani goats, especially Sahrawi goats, can increase growth performance, as well as improve fatty acid composition and meat quality. Full article
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14 pages, 1742 KiB  
Article
Integrative Analysis of Blood Transcriptomics and Metabolomics Reveals Molecular Regulation of Backfat Thickness in Qinchuan Cattle
by Hengwei Yu, Sayed Haidar Abbas Raza, Yueting Pan, Gong Cheng, Chugang Mei and Linsen Zan
Animals 2023, 13(6), 1060; https://doi.org/10.3390/ani13061060 - 15 Mar 2023
Cited by 1 | Viewed by 1321
Abstract
A crucial goal of reducing backfat thickness (BFT) is to indirectly improve feed conversion efficiency. This phenotype has been reported in certain papers; however, the molecular mechanism has yet to be fully revealed. Two extreme BFT groups, consisting of four Qinchuan cattle, were [...] Read more.
A crucial goal of reducing backfat thickness (BFT) is to indirectly improve feed conversion efficiency. This phenotype has been reported in certain papers; however, the molecular mechanism has yet to be fully revealed. Two extreme BFT groups, consisting of four Qinchuan cattle, were chosen for this study. We performed metabolite and transcriptome analyses of blood from cattle with a high BFT (H-BFT with average = 1.19) and from those with a low BFT (L-BFT with average = 0.39). In total, 1106 differentially expressed genes (DEGs) and 86 differentially expressed metabolites (DEMs) were identified in the extreme trait. In addition, serum ceramide was strongly correlated with BFT and could be used as a potential biomarker. Moreover, the most notable finding was that the functional genes (SMPD3 and CERS1) and metabolite (sphingosine 1-phosphate (S1P)) were filtered out and significantly enriched in the processes related to the sphingolipid metabolism. This investigation contributed to a better understanding of the subcutaneous fat depots in cattle. In general, our results indicated that the sphingolipid metabolism, involving major metabolites (serum ceramide and S1P) and key genes (SMPD3 and CERS1), could regulate BFT through blood circulation. Full article
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11 pages, 476 KiB  
Article
Eating Quality of Pork from Entire Male Pigs after Dietary Supplementation with Hydrolysable Tannins
by Ivan Bahelka, Roman Stupka, Jaroslav Čítek, Michal Šprysl, Ondřej Bučko and Pavel Fľak
Animals 2023, 13(5), 893; https://doi.org/10.3390/ani13050893 - 1 Mar 2023
Viewed by 1533
Abstract
Boar taint is an unpleasant odour and flavour released during heat treatment of pork from uncastrated male pigs. The two main compounds responsible for boar taint are androstenone and skatole. Androstenone is a steroid hormone formed in the testis during sexual maturity. Skatole [...] Read more.
Boar taint is an unpleasant odour and flavour released during heat treatment of pork from uncastrated male pigs. The two main compounds responsible for boar taint are androstenone and skatole. Androstenone is a steroid hormone formed in the testis during sexual maturity. Skatole is a product of microbial degradation of the amino acid tryptophan in the hindgut of pigs. Both of these compounds are lipophilic, which means that they can be deposited in adipose tissue. Several studies have reported heritability estimates for their deposition from medium (skatole) to high magnitudes (androstenone). In addition to efforts to influence boar taint through genetic selection, much attention has also been paid to reducing its incidence using various feeding strategies. From this point of view, research has focused especially on the reduction in skatole content by supplementation of feed additives into the nutrition of entire male pigs. Promising results have been achieved using hydrolysable tannins in the diet. To date, most studies have investigated the effects of tannins on the production and accumulation of skatole in adipose tissue, intestinal microbiota, growth rate, carcasses and pork quality. Thus, the objective of this study was, in addition to determining the effects of tannins on androstenone and skatole accumulation, to assess the effects of tannins on the sensory traits of meat from entire males. The experiment was performed on 80 young boars—progeny of several hybrid sire lines. Animals were randomly assigned to one control and four experimental groups (each numbering 16). The control group (T0) received a standard diet without any tannin supplementation. Experimental groups were supplemented with 1% (T1), 2% (T2), 3% (T3) or 4% (T4) SCWE (sweet chestnut wood extract) rich in hydrolysable tannins (Farmatan). Pigs received this supplement for 40 days prior to slaughter. Subsequently, the pigs were slaughtered, and sensory analysis was applied to evaluate the odour, flavour, tenderness and juiciness of the pork. The results showed a significant effect of tannins on skatole accumulation in adipose tissue (p = 0.052–0.055). The odour and flavour of the pork were not affected by tannins. However, juiciness and tenderness were reduced by higher tannin supplementation (T3–T4) compared to the controls (p < 0.05), but these results were sex-dependent (in favour of men compared to women). Generally, women rated tenderness and juiciness worse than men regardless of the type of diet. Full article
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