Mechanisms of Biofilm Development and Antibiofilm Therapeutics Strategies

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Antibiofilm Strategies".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 1033

Special Issue Editors


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Guest Editor
Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland
Interests: biofilms; biofilm-related infections; microbial resistance; probiotics; antibiotic stewardship; oral health; oral microbiome

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Guest Editor
Biotechnology Centre, The Silesian University of Technology, Gliwice, Poland
Interests: pharmaceuticals degradation; antibiotics resistance; biofilm development; bacterial metabolism; immobilization; 3D printing; engineered living materials

Special Issue Information

Dear Colleagues,

Biofilms have been found to play a key role in infections, various diseases, and implant failure. Treatment of biofilm-associated infections imposes many challenging tasks on microbiologists and clinicians. Recent decades of impressive discoveries have yielded insightful knowledge about the structure and complexity of biofilm and deepened our understanding of various aspects of biofilm pathogenesis. Microorganisms can adhere to various types of organic and abiotic surfaces, so a better understanding of the stages of biofilm formation requires an interdisciplinary approach that leads to the development of effective alternatives for prevention and control. Research and new strategies to prevent and combat biofilm are a dynamically developing branch of science. This Special Issue’s outlook is to address current biofilm-targeting strategies and those under development with a special focus on new molecules and technologies with antibiofilm capabilities. It will expand and systematize the knowledge regarding the dynamic of biofilm formation and summarize ongoing efforts related to the development and understanding of the main concerns in fungal and bacterial biofilms.

Dr. Ruth Dudek-Wicher
Dr. Joanna Żur
Guest Editors

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Keywords

  • biofilm
  • polymicrobial biofilms
  • biofilm development
  • biofilm eradication
  • antibiofilm activity
  • targeted anti-biofilm therapy
  • EPS
  • quorum sensing
  • metabolic
  • AMP
  • biofilm penetration
  • enhanced antimicrobial therapy
  • multi-immunotherapy

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Published Papers (1 paper)

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Research

16 pages, 2663 KiB  
Article
Biofilm Formation and Antibiotic Resistance Profiles in Carbapenemase-Producing Gram-Negative Rods—A Comparative Analysis between Screening and Pathological Isolates
by Camelia Vintilă, Răzvan Lucian Coșeriu, Anca Delia Mare, Cristina Nicoleta Ciurea, Radu Ovidiu Togănel, Anastasia Simion, Anca Cighir and Adrian Man
Antibiotics 2024, 13(8), 687; https://doi.org/10.3390/antibiotics13080687 - 24 Jul 2024
Viewed by 830
Abstract
(1) Background: Carbapenem-resistant (CR) bacteria pose a significant global public health challenge due to their ability to evade treatment with beta-lactam antibiotics, including carbapenems. This study investigates the biofilm-forming capabilities of CR clinical bacterial isolates and examines the impact of serum on biofilm [...] Read more.
(1) Background: Carbapenem-resistant (CR) bacteria pose a significant global public health challenge due to their ability to evade treatment with beta-lactam antibiotics, including carbapenems. This study investigates the biofilm-forming capabilities of CR clinical bacterial isolates and examines the impact of serum on biofilm formation. Additionally, the study evaluates the resistance profiles and genetic markers for carbapenemase production. (2) Methods: Bacterial isolates were collected from the microbiology laboratory of Mures County Clinical Hospital between October 2022 and September 2023. Pharyngeal and rectal swabs were screened for carbapenem-resistant bacteria using selective media. Lower respiratory tract samples were also analyzed for CR Gram-negative bacteria. The isolates were tested for their ability to form biofilms in the presence and absence of fetal bovine serum at 24 and 48 h. Carbapenemase production was detected phenotypically and confirmed via PCR for relevant genes. (3) Results: Out of 846 screened samples, 4.25% from pharyngeal swabs and 6.38% from rectal swabs tested positive for CR bacteria. Acinetobacter baumannii and Klebsiella pneumoniae were the most common species isolated. Biofilm formation varied significantly between clinical isolates and standard strains, with clinical isolates generally showing higher biofilm production. The presence of serum had no significant effect on biofilm formation in Klebsiella spp., but stimulated biofilm formation for Acinetobacter spp. Carbapenemase genes blaKPC, blaOXA-48-like, and blaNDM were detected in various isolates, predominantly in Klebsiella spp., but were not the main determinants of carbapenem resistance, at least in screening isolates. (4) Conclusions: This study highlights the variability in biofilm formation among CR clinical isolates and underscores the differences between the bacteria found as carriage versus infection. Both bacterial species and environmental factors variably influence biofilm formation. These insights are crucial for the development of effective treatment and infection control strategies in clinical settings. Full article
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