Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.3
2.5
Journals
Applied Sciences
Special Issues
Novel HPLC-Assisted Method for Investigation of the Antioxidant Activity of...
applsci-logo
Submit to Applied Sciences Review for Applied Sciences Propose a Special Issue

Journal Menu

► Journal Menu

Journal Browser

► Journal Browser
share announcement

Novel HPLC-Assisted Method for Investigation of the Antioxidant Activity of Plant Extracts

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (10 December 2021) | Viewed by 8341

Special Issue Editor

Dr. Nina Kashchenko

E-Mail Website
Guest Editor
Institute of General and Experimental Biology, Siberian Division of Russian Academy of Science, 6, 670047 Ulan-Ude, Russia
Interests: phytochemicals; phenolic compounds; ellagitannins; flavonoids; HPLC
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Various phytochemicals and bioactive compounds are sourced from medicinal plants and have an impact on pharmaceutical, nutraceutical, and functional food industries. Many of these secondary plant metabolites are explored from initial identification to advanced clinical research before officially being used as pharmaceutical and nutraceutical ingredients. The development of advanced tools for the qualitative and quantitative assessment of phytochemicals, such as high-performance liquid chromatography and liquid chromatography–mass spectrometry, has enhanced the rate of bioassay-guided fractionation, significantly improving phytochemical investigation. Particular attention has been devoted to the creation of convenient and reliable methods for studying the antioxidant activity of plant extracts. A relatively new development in this field is the HPLC-assisted techniques, combining chromatographic analysis and pre-/post-column derivatization of the sample with the use of various agents, such as DPPH-HPLC or ABTS-HPLC, thereby avoiding the expensive isolation and purification steps. Given the special interest in the investigation of antioxidant activity, a promising task is the creation of correct, rapid, and inexpensive HPLC methods to determine individual compounds of plant extracts in on-line conditions. Therefore, contributions to this Special Issue may cover all research aspects related to medicinal, aromatic, and edible plant extracts; development of HPLC-assisted methods for investigation of the antioxidant activity of plant extracts; characterization of investigated plant extracts, including (but not limited to) methods for their extraction, purification, comprehensive profiling characterization, and the quantification and elucidation of their mechanisms of action with a focus on antioxidant activity.

Dr. Nina Kashchenko
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant extracts
  • phytochemicals
  • HPLC
  • antioxidant activity
  • DPPH
  • ABTS
  • bioassay-guided techniques

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

14 pages, 7754 KiB  
Article
Phytohormones and Elicitors Enhanced the Ecdysteroid and Glycosylflavone Content and Antioxidant Activity of Silene repens
by Nina I. Kashchenko, Daniil N. Olennikov and Nadezhda K. Chirikova
Appl. Sci. 2021, 11(23), 11099; https://doi.org/10.3390/app112311099 - 23 Nov 2021
Cited by 9 | Viewed by 1869
Abstract
In the course of the ongoing chemical study of species of Silene genus, S. repens Patrin as a common species of the genus, was selected as the object of this study. Using high-performance liquid chromatography with photodiode array detection and electrospray ionization triple [...] Read more.
In the course of the ongoing chemical study of species of Silene genus, S. repens Patrin as a common species of the genus, was selected as the object of this study. Using high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS), the presence of 12 ecdysteroids and 6 glycosylflavones was established in S. repens introduced seedlings. 20-Hydroxyecdysone and polypodine B, as well as sileneside E and schaftoside, were the dominant compounds in introduced seedlings of S. repens. The effect of exogenous phytohormones and elicitors on the productivity and accumulation of ecdysteroids and glycosylflavones in introduced seedlings of S. repens was investigated for the first time. It was found that the use of ethyl arachidonate (100 mg/L) to increase the productivity of S. repens is justified. To obtain S. repens with a high content of ecdysteroids and glycosylflavones, it is recommended to apply epibrassinolide (100 mg/L) and 4-chlorophenoxyacetic acid (100 mg/L), respectively. Antioxidant activity of S. repens against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) was determined, and it was revealed that sileneside E and schaftoside, as well as meloside A and isovitexin had the highest antioxidant activity among the studied compounds of S. repens. Full article
(This article belongs to the Special Issue Novel HPLC-Assisted Method for Investigation of the Antioxidant Activity of Plant Extracts)
►▼ Show Figures

Figure 1

12 pages, 288 KiB  
Article
Antioxidant Activity and Bioactive Compounds of Lamium album Flower Extracts Obtained by Supercritical Fluid Extraction
by Pascaline Aimee Uwineza, Anna Gramza-Michałowska, Marcin Bryła and Agnieszka Waśkiewicz
Appl. Sci. 2021, 11(16), 7419; https://doi.org/10.3390/app11167419 - 12 Aug 2021
Cited by 23 | Viewed by 3238
Abstract
In this research, supercritical CO2 extraction is applied to extract bioactive compounds from Lamium album (white dead nettle, Lamiaceae). Extraction was performed at various temperatures (40, 50, and 60 °C) using methanol as co-solvent at a constant flow rate of CO [...] Read more.
In this research, supercritical CO2 extraction is applied to extract bioactive compounds from Lamium album (white dead nettle, Lamiaceae). Extraction was performed at various temperatures (40, 50, and 60 °C) using methanol as co-solvent at a constant flow rate of CO2, methanol, and pressure. The collected extracts were characterized in terms of antioxidant capacity by using DPPH, ABTS and FRAP in vitro antioxidant activity assays, whereas the Folin–Ciocalteu procedure was employed to estimate the total phenols content (TPC). On the other hand, phenolic compounds in the extracts were quantitated by liquid chromatography coupled with a photodiode array detector (UPLC-PDA) and confirmed with a mass detector (TQD). The extracts have shown high TPC ranged between 234.17 to 650.17 mg GAE/g extract. DPPH scavenging of the extracts was estimated and obtained EC50 values ranged from 0.12 to 0.37 mg/mL of solution. The ABTS radical scavenging activity ranged from 43.20 to 44.53 µg TE/g. The FRAP value was found within the range of 19.48 to 44.74 µmol TE/g of extract. Differences between extraction conditions were observed. In this research, 50 °C/250 bar was efficient for the TPC, DPPH, ABTS, and FRAP assays; moreover, statistically, TPCs and FRAP assay showed significant differences between the conditions at α = 0.05. The identification of phenolic compounds in the obtained extract of Lamium album flowers, using UPLC/PDA, revealed that chrysin, pinostrobin, myricetin, and trans-3-hydroxycinnamic acid were the significant molecules present, which may be responsible for the high content of polyphenols and antioxidant activity. The results obtained indicated that SC-CO2 could be considered an alternative method for extracting bioactive compounds of Lamium album. High antioxidant activity and the presence of various bioactive compounds indicate the potential of this plant from the Lamiaceae family and the possibility of its application in various industries, including agriculture, food technology, or pharmacy. Full article
(This article belongs to the Special Issue Novel HPLC-Assisted Method for Investigation of the Antioxidant Activity of Plant Extracts)
8 pages, 947 KiB  
Article
Fast HILIC Method for Separation and Quantification of Non-Volatile Aromatic Compounds and Monosaccharides from Willow (Salix sp.) Bark Extract
by Heidi Meriö-Talvio, Jinze Dou, Tapani Vuorinen and Leena Pitkänen
Appl. Sci. 2021, 11(9), 3808; https://doi.org/10.3390/app11093808 - 23 Apr 2021
Cited by 2 | Viewed by 2485
Abstract
Willow bark water extracts contain a mixture of chemically heterogeneous compounds. Fast screening techniques of the extracts are often needed to obtain information on the profile of bioactive and/or other valuable components in the extract. This is, however, a challenging task due to [...] Read more.
Willow bark water extracts contain a mixture of chemically heterogeneous compounds. Fast screening techniques of the extracts are often needed to obtain information on the profile of bioactive and/or other valuable components in the extract. This is, however, a challenging task due to the different chemical structures of the components. Willow bark extract from the hybrid Karin contains several bioactive compounds such as aromatic picein, triandrin, and (+)-catechin. Willow bark extract also contains significant amounts of the monosaccharides fructose and glucose. Here, we demonstrate the applicability of hydrophilic interaction liquid chromatography, coupled with evaporative light scattering and ultraviolet detectors, for the simultaneous separation and quantification of major aromatic compounds and monosaccharides from the willow bark extract. The ternary eluent mixture consisting of acetonitrile, water, and methanol enabled the baseline separation of the main components in the extract in a short analysis time, which makes this method ideal for fast screening of the plant extracts and investigating the purity of fractionated bioactive compounds. Full article
(This article belongs to the Special Issue Novel HPLC-Assisted Method for Investigation of the Antioxidant Activity of Plant Extracts)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop