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Applied Sciences
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Cold Atmospheric Plasma and Its Applications
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Cold Atmospheric Plasma and Its Applications

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (20 October 2022) | Viewed by 4006

Special Issue Editors

Prof. Dr. Andrea Sevcovicova

E-Mail Website
Guest Editor
Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
Interests: genetics; genotoxicology; DNA damage; mutagenicity; antioxidants, phytocompounds; cold atmospheric plasma
Dr. Stanislav Kyzek

E-Mail Website
Guest Editor
Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
Interests: comet assay; genotoxicity; DNA damage; mutagenicity; genetic toxicology; mutagenesis; toxicity; mutation; reactive oxygen species; DNA

Special Issue Information

Dear Colleagues,

Cold atmospheric plasma has been widely used in many industrial fields. However, in the last several decades it has been the subject of many studies aimed at evaluating its effects on biological material. Among these effects, the most studied are decontamination effects with potential use mainly in medicine (stomatology, dermatology, oncology) and agriculture. Moreover, cold atmospheric plasma can be applied directly on the surface or indirectly using plasma-activated water or medium which allows its application also to the inner layers of treated material. However, the effects of cold atmospheric plasma treatment differ due to the use of different plasma sources and treatment conditions in various laboratories. Therefore, further studies aimed at elucidating the mechanisms involved in plasma and treated object interactions are required. This Special Issue calls for original articles and reviews devoted to the application of cold atmospheric plasma in various research fields. Its aim is to highlight discoveries and comprehensive approaches in research on the plasma effects with potential use in different fields of human life.

Prof. Dr. Andrea Sevcovicova
Dr. Stanislav Kyzek
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • cold atmospheric plasma
  • plasma application
  • plasma-activated solutions
  • plasma interactions
  • plasma source

Published Papers (2 papers)

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Research

19 pages, 4763 KiB  
Article
Low-Dose Non-Thermal Atmospheric Plasma Promotes the Proliferation and Migration of Human Normal Skin Cells
by Hui Wu, Yan Zhang, Yuanyuan Zhou, Zhuna Yan, Jinwu Chen, Tingting Lu and Wencheng Song
Appl. Sci. 2023, 13(5), 2866; https://doi.org/10.3390/app13052866 - 23 Feb 2023
Cited by 4 | Viewed by 1406
Abstract
(1) Background: The purpose of this study was to investigate the effects of low-dose non-thermal atmospheric plasma (NTP) on the proliferation and migration of human immortalized keratinocytes (HaCaT cells) and its molecular mechanisms. (2) Methods: The effects of NTP on HaCaT cells were [...] Read more.
(1) Background: The purpose of this study was to investigate the effects of low-dose non-thermal atmospheric plasma (NTP) on the proliferation and migration of human immortalized keratinocytes (HaCaT cells) and its molecular mechanisms. (2) Methods: The effects of NTP on HaCaT cells were detected by cell viability, wound healing assay, cell cycle, mitochondrial membrane potential detection, and western blot, and the role of reactive oxygen species (ROS) content in low-dose NTP was explored. (3) Results: Results showed that the proportion of cells in G1-S phase transition, cell migration ability, and the expression of Cyclin D1 and STAT3 reached the peak at 10 s treatment group, while the cell viability and the expression of PI3K, AKT, mTOR, ERK, WNT, and β-catenin proteins reached the peak at 15 s treatment group. (4) Conclusions: These results manifested that ROS produced by low-dose NTP promoted the proliferation of HaCaT cells by activating the PI3K/AKT/mTOR signaling pathway and also promoted the migration by activating the WNT/β-catenin signaling pathway. Therefore, these results will be useful for the application of low-dose NTP in the treatment of wound healing. Full article
(This article belongs to the Special Issue Cold Atmospheric Plasma and Its Applications)
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16 pages, 2882 KiB  
Article
Low Temperature Plasma Strategies for Xylella fastidiosa Inactivation
by Paolo Francesco Ambrico, Stefania Zicca, Marianna Ambrico, Palma Rosa Rotondo, Angelo De Stradis, Giorgio Dilecce, Maria Saponari, Donato Boscia and Pasquale Saldarelli
Appl. Sci. 2022, 12(9), 4711; https://doi.org/10.3390/app12094711 - 7 May 2022
Cited by 3 | Viewed by 1956
Abstract
The quarantine bacterium Xylella fastidiosa was first detected in Salento (Apulia, Italy) in 2013 and caused severe symptoms in olives, leading to plant death. The disease, named Olive Quick Decline Syndrome (OQDS), is caused by the strain “De Donno” ST53 of the subspecies [...] Read more.
The quarantine bacterium Xylella fastidiosa was first detected in Salento (Apulia, Italy) in 2013 and caused severe symptoms in olives, leading to plant death. The disease, named Olive Quick Decline Syndrome (OQDS), is caused by the strain “De Donno” ST53 of the subspecies pauca of this bacterium (XfDD), which is spread by the insect Philaenus spumarius. The epidemic poses a serious threat to the agricultural economy and the landscape, as X. fastidiosa infects several plant species and there is yet no recognized solution. Research on OQDS is focused on finding strategies to control its spread or mitigate its symptoms. As a perspective solution, we investigated the efficacy of the low-temperature plasma and plasma-activated water to kill bacterial cells. Experiments were conducted in vitro to test the biocidal effect of the direct application of a Surface Dielectric Barrier Discharge (SDBD) plasma on bacteria cells and Plasma Activated Water (PAW). PAW activity was tested as a possible biocidal agent that can move freely in the xylem network paving the way to test the strategy on infected plants. The results showed a high decontamination rate even for cells of XfDD embedded in biofilms grown on solid media and complete inactivation in liquid culture medium. Full article
(This article belongs to the Special Issue Cold Atmospheric Plasma and Its Applications)
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