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Biology
Special Issues
Applications of Proteomics in Biological Fluids and Biopsies
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Applications of Proteomics in Biological Fluids and Biopsies

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Medical Biology".

Deadline for manuscript submissions: 30 June 2025 | Viewed by 1947

Special Issue Editor

Prof. Dr. Youhe Gao

E-Mail Website
Guest Editor
Gene Engineering Drug and Biotechnology Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing 100875, China
Interests: urinary biomarker; early diagnosis; proteomics; biochemistry; biobanking
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Samples from biological fluids and biopsies are very important for understanding the biology and pathophysiology of diseases. Proteomics is a tool that we can use for certain "disease-specific protein molecules" that could be molecular targets for new drug designs or provide molecular markers for the early diagnosis of diseases. The journal Biology is launching a Special Issue entitled "Applications of Proteomics in Biological Fluids and Biopsies”, which promotes basic research, translation, and clinical applications in the application of proteomics in samples including urine, blood, other biological fluids, and biopsies.

Prof. Dr. Youhe Gao
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • proteomics
  • biological fluids
  • biomarkers
  • biopsies
  • disease
  • diagnosis
  • mechanisms

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Published Papers (1 paper)

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Research

15 pages, 4251 KiB  
Article
Proteomic Analysis Identifies Dysregulated Proteins in Albuminuria: A South African Pilot Study
by Siyabonga Khoza, Jaya A. George, Previn Naicker, Stoyan H. Stoychev, June Fabian and Ireshyn S. Govender
Biology 2024, 13(9), 680; https://doi.org/10.3390/biology13090680 - 30 Aug 2024
Viewed by 1436
Abstract
Albuminuria may precede decreases in the glomerular filtration rate (GFR) and both tests are insensitive predictors of early stages of kidney disease. Our aim was to characterise the urinary proteome in black African individuals with albuminuria and well-preserved GFR from South Africa. This [...] Read more.
Albuminuria may precede decreases in the glomerular filtration rate (GFR) and both tests are insensitive predictors of early stages of kidney disease. Our aim was to characterise the urinary proteome in black African individuals with albuminuria and well-preserved GFR from South Africa. This case-controlled study compared the urinary proteomes of 52 normoalbuminuric (urine albumin: creatinine ratio (uACR) < 3 mg/mmol) and 56 albuminuric (uACR ≥ 3 mg/mmol) adults of black African ethnicity. Urine proteins were precipitated, reduced, alkylated, digested, and analysed using an Evosep One LC (Evosep Biosystems, Odense, Denmark) coupled to a Sciex 5600 Triple-TOF (Sciex, Framingham, MA, USA) in data-independent acquisition mode. The data were searched on SpectronautTM 15. Differentially abundant proteins (DAPs) were filtered to include those with a ≥2.25-fold change and a false discovery rate ≤ 1%. Receiver–operating characteristic curves were used to assess the discriminating abilities of proteins of interest. Pathway analysis was performed using Enrichr software. As expected, the albuminuric group had higher uACR (7.9 vs. 0.55 mg/mmol, p < 0.001). The median eGFR (mL/min/1.73 m2) showed no difference between the groups (111 vs. 114, p = 0.707). We identified 80 DAPs in the albuminuria group compared to the normoalbuminuria group, of which 59 proteins were increased while 21 proteins were decreased in abundance. We found 12 urinary proteins with an AUC > 0.8 and a p < 0.001 in the multivariate analysis. Furthermore, an 80-protein model was developed that showed a high AUC ˃ 0.907 and a predictive accuracy of 91.3% between the two groups. Pathway analysis found that the DAPs were involved in insulin growth factor (IGF) functions, innate immunity, platelet degranulation, and extracellular matrix organization. In albuminuric individuals with a well-preserved eGFR, pathways involved in preventing the release and uptake of IGF by insulin growth factor binding protein were significantly enriched. These proteins are indicative of a homeostatic imbalance in a variety of cellular processes underlying renal dysfunction and are implicated in chronic kidney disease. Full article
(This article belongs to the Special Issue Applications of Proteomics in Biological Fluids and Biopsies)
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