Microbial Response to Stresses, Phages and Hosts

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Microbiology".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 2051

Special Issue Editors

Department of Biology, University of Copenhagen, København, Denmark
Interests: signal transduction; stress response; second messenger; defense and anti-defense systems

E-Mail Website
Guest Editor
Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
Interests: bacterial pathogenesis; stress response; gene regulation; small noncoding RNA; RNA-binding proteins; RNA-seq; RNA interactome

Special Issue Information

Dear Colleagues,

Bacteria face various environmental stresses, such as changes in temperature, pH, and nutrient availability, as well as exposure to antibiotics, phages, and host immune systems. Bacteria have evolved various mechanisms to cope with these stresses, including changes in gene expression, the production of protective molecules, and the activation of stress response pathways. Understanding these mechanisms is crucial for developing strategies to control bacterial infections and improve health outcomes.

Recent research has revealed new mechanisms of bacterial stress responses and their interactions with phages and host cells. Examples include novel second messengers, such as pGpp and AppppA, and their target proteins in the bacterial adaptation to stresses; riboregulation involving various noncoding RNAs and their chaperone; the role of bacterial antitoxin–toxins in inducing an abortive infection of phages; the race between phages and bacteria via the CRISPR and anti-CRISPR systems; the cGAS–cGAMP axis in both eukaryotic and bacterial cell immune responses; etc.

Excited by these advances, this Special Issue aims to encourage researchers to share their recent understandings of bacterial stress responses and bacterial interactions with phages and host cells through various mechanisms. We welcome original research and review articles on the following topics, including, but not limited to:

  • Bacterial response pathways to abiotic stresses and antibiotics;
  • Bacterial interactions with phages and host immune systems;
  • Second messengers, small noncoding RNAs, and two-component systems;
  • Biofilm, quorum sensing, and microbiota–host interactions;
  • Application of bacterial stress response in biotechnology and agriculture.

Dr. Yong Zhang
Dr. Yanjie Chao
Guest Editors

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Keywords

  • bacterial stress response
  • second messenger
  • phage
  • host immune system
  • small noncoding RNA
  • toxin
  • anti-biotics

Published Papers (1 paper)

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Research

21 pages, 7520 KiB  
Article
DNA-Binding Protein Dps Protects Escherichia coli Cells against Multiple Stresses during Desiccation
by Nataliya Loiko, Ksenia Tereshkina, Vladislav Kovalenko, Andrey Moiseenko, Eduard Tereshkin, Olga S. Sokolova and Yurii Krupyanskii
Biology 2023, 12(6), 853; https://doi.org/10.3390/biology12060853 - 14 Jun 2023
Cited by 3 | Viewed by 1739
Abstract
Gradual dehydration is one of the frequent lethal yet poorly understood stresses that bacterial cells constantly face in the environment when their micro ecotopes dry out, as well as in industrial processes. Bacteria successfully survive extreme desiccation through complex rearrangements at the structural, [...] Read more.
Gradual dehydration is one of the frequent lethal yet poorly understood stresses that bacterial cells constantly face in the environment when their micro ecotopes dry out, as well as in industrial processes. Bacteria successfully survive extreme desiccation through complex rearrangements at the structural, physiological, and molecular levels, in which proteins are involved. The DNA-binding protein Dps has previously been shown to protect bacterial cells from many adverse effects. In our work, using engineered genetic models of E. coli to produce bacterial cells with overproduction of Dps protein, the protective function of Dps protein under multiple desiccation stresses was demonstrated for the first time. It was shown that the titer of viable cells after rehydration in the experimental variants with Dps protein overexpression was 1.5–8.5 times higher. Scanning electron microscopy was used to show a change in cell morphology upon rehydration. It was also proved that immobilization in the extracellular matrix, which is greater when the Dps protein is overexpressed, helps the cells survive. Transmission electron microscopy revealed disruption of the crystal structure of DNA–Dps crystals in E. coli cells that underwent desiccation stress and subsequent watering. Coarse-grained molecular dynamics simulations showed the protective function of Dps in DNA–Dps co-crystals during desiccation. The data obtained are important for improving biotechnological processes in which bacterial cells undergo desiccation. Full article
(This article belongs to the Special Issue Microbial Response to Stresses, Phages and Hosts)
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