Prof. Shishmon Belkin 30th Anniversary of Working in Bioluminescent Bioreporter Bacteria Biosensor

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 711

Special Issue Editors


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Guest Editor
Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer-Sheva 8410500, Israel
Interests: biosensors; diagnostics; biomaterials; environmental toxicity; drug discovery; vaccines
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Guest Editor
School of Materials and Energy, University of Electronic Science and Technology of China, 611731 Chengdu, China
Interests: thin film sensor; immunsensor; raman; polymer materials

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Department of Postharvest Science ARO, Institute of Postharvest and Food Sciences, The Volcani Center HaMaccabim Rd 68, POB 15159, Rishon LeZion 7528809, Israel
Interests: bioluminescent bacterial; point-of-care devices; environmental; nano-based biosensors; real-time monitoring systems

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Guest Editor
Drexel University College of Medicine, Philadelphia, PA, USA
Interests: biological sensing; immunoassays; drug delivery

Special Issue Information

Dear Colleagues,

The Special Issue themed after retired Prof. Shishmon Belkin to honour his past accomplishments in the field will include various bodies of work such as biography, reviews, original research, target product profiles (new paper concept usually found in business plans), etc.… The topics covered are applications using bioluminescent bioreporter bacteria in screening or monitoring, adapted, or not, to a transducer configuration, thus tailored to be part of a biosensor system. Applications can be found in environmental toxicology, biotechnology engineering, information engineering (to understand complex mixtures), 3D bioinks, clinical applications, and more.

Prof. Dr. Robert Steven Marks
Prof. Dr. Kun Jia
Dr. Evgeni Eltzov
Dr. Boris Polyak
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioluminescence
  • bioreporters
  • biosensors
  • applications
  • commercialization

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Published Papers (1 paper)

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Research

22 pages, 8826 KiB  
Article
Microbead-Encapsulated Luminescent Bioreporter Screening of P. aeruginosa via Its Secreted Quorum-Sensing Molecules
by Abraham Abbey Paul, Yael Schlichter Kadosh, Ariel Kushmaro and Robert S. Marks
Biosensors 2024, 14(8), 383; https://doi.org/10.3390/bios14080383 - 8 Aug 2024
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Abstract
Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that remains a prevalent clinical and environmental challenge. Quorum-sensing (QS) molecules are effective biomarkers in pinpointing the presence of P. aeruginosa. This study aimed to develop a convenient-to-use, whole-cell biosensor using P. aeruginosa reporters individually [...] Read more.
Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that remains a prevalent clinical and environmental challenge. Quorum-sensing (QS) molecules are effective biomarkers in pinpointing the presence of P. aeruginosa. This study aimed to develop a convenient-to-use, whole-cell biosensor using P. aeruginosa reporters individually encapsulated within alginate-poly-L-lysine (alginate-PLL) microbeads to specifically detect the presence of bacterial autoinducers. The PLL-reinforced microbeads were prepared using a two-step method involving ionic cross-linking and subsequent coating with thin layers of PLL. The alginate-PLL beads showed good stability in the presence of a known cation scavenger (sodium citrate), which typically limits the widespread applications of calcium alginate. In media containing synthetic autoinducers—such as N-(3-oxo dodecanoyl) homoserine lactone (3-oxo-C12-HSL) and N-butanoyl-L-homoserine lactone (C4-HSL), or the cell-free supernatants of planktonic or the flow-cell biofilm effluent of wild P. aeruginosa (PAO1)—the encapsulated bacteria enabled a dose-dependent detection of the presence of these QS molecules. The prepared bioreporter beads remained stable during prolonged storage at 4 and −80 °C and were ready for on-the-spot sensing without the need for recovery. The proof-of-concept, optical fiber-based, and whole-cell biosensor developed here demonstrates the practicality of the encapsulated bioreporter for bacterial detection based on specific QS molecules. Full article
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